Lipin 2 (LPIN2) (NM_014646) Human Tagged ORF Clone

CAT#: RC224070

LPIN2 (Myc-DDK-tagged)-Human lipin 2 (LPIN2)

Product Images

Circular map for RC224070

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPIN2 (Cat# RC224070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPIN2(Cat# TA590249). Positive lysates LY402358 (100ug) and LC402358 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402358) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224070 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LPIN2 protein (Cat# TP324070). The protein was produced from HEK293T cells transfected with LPIN2 cDNA clone (Cat# RC224070) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipin 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224070 representing NM_014646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTATGTGGGACAGCTGGCTGGGCAGGTGATTGTCACTGTGAAGGAACTCTACAAGGGCATTAACC
  AGGCCACCCTCTCTGGGTGCATTGATGTCATCGTGGTACAGCAGCAGGATGGCAGCTATCAGTGTTCACC
  TTTTCACGTTCGGTTTGGAAAGCTGGGAGTCCTGAGATCCAAAGAGAAAGTGATTGATATAGAAATCAAC
  GGCAGTGCAGTGGATCTTCACATGAAGTTGGGTGATAACGGAGAAGCTTTCTTTGTTGAGGAGACTGAAG
  AAGAATATGAAAAGCTTCCTGCTTACCTTGCCACCTCACCAATTCCTACTGAAGATCAGTTCTTTAAAGA
  TATTGACACCCCTTTGGTGAAATCGGGTGGAGATGAAACACCATCTCAGAGTTCAGACATCTCACACGTC
  TTGGAAACAGAGACAATTTTTACTCCAAGTTCTGTGAAAAAGAAAAAACGAAGGAGAAAGAAATACAAAC
  AGGACAGTAAGAAGGAAGAGCAGGCCGCATCTGCTGCTGCAGAAGACACATGTGATGTAGGCGTGAGCTC
  CGATGATGACAAGGGGGCCCAGGCAGCACGAGGATCTTCAAATGCTTCCTTGAAAGAAGAAGAATGTAAA
  GAGCCTTTGCTCTTCCATTCTGGGGATCATTACCCCTTATCTGATGGAGATTGGTCCCCTTTAGAGACCA
  CCTATCCCCAGACAGCGTGTCCTAAGAGTGATTCAGAGCTGGAGGTGAAACCTGCGGAGAGCCTGCTCAG
  ATCAGAGTCTCACATGGAGTGGACGTGGGGCGGATTCCCAGAGTCCACCAAGGTCAGCAAAAGAGAACGA
  TCTGACCATCATCCTAGGACAGCTACAATTACACCATCAGAAAATACTCATTTTCGGGTAATTCCCAGTG
  AGGACAACCTCATCAGTGAAGTTGAGAAGGATGCTTCCATGGAAGACACTGTCTGTACCATAGTGAAGCC
  CAAACCCAGAGCCCTGGGTACACAGATGAGCGACCCAACATCTGTGGCAGAGCTTCTCGAACCTCCTCTT
  GAGAGTACTCAGATTTCATCTATGTTAGATGCTGACCACCTTCCCAACGCAGCCTTAGCGGAGGCGCCCT
  CAGAATCCAAACCGGCAGCTAAAGTAGACTCGCCGTCAAAGAAGAAAGGTGTTCACAAAAGAAGCCAACA
  CCAGGGACCTGATGATATTTACCTTGATGACTTAAAGGGTCTAGAACCTGAAGTTGCAGCTCTTTATTTC
  CCTAAAAGTGAATCGGAGCCCGGTTCCAGGCAGTGGCCCGAGTCTGACACACTCTCTGGCTCCCAGTCCC
  CACAGTCCGTGGGAAGCGCAGCTGCAGATAGCGGCACCGAGTGCCTCTCAGATTCTGCCATGGACTTGCC
  TGACGTTACCCTCTCCCTTTGCGGGGGCCTCAGTGAAAATGGAGAAATTTCAAAAGAAAAATTCATGGAG
  CATATCATTACTTATCACGAATTTGCAGAAAACCCTGGACTTATAGACAATCCTAACCTTGTAATAAGGA
  TATATAATCGTTACTATAACTGGGCTTTGGCAGCTCCCATGATCCTTAGCTTGCAAGTATTCCAGAAGAG
  CTTGCCTAAGGCCACAGTTGAGTCCTGGGTGAAAGACAAGATGCCAAAGAAATCTGGTCGCTGGTGGTTT
  TGGCGAAAGAGAGAAAGCATGACCAAACAGCTGCCAGAATCCAAGGAGGGAAAATCTGAGGCACCGCCAG
  CCAGTGACCTGCCATCCAGCTCCAAGGAGCCGGCCGGTGCCAGGCCGGCCGAGAATGACTCCTCGAGTGA
  CGAGGGATCACAGGAGCTCGAAGAATCCATCACAGTGGACCCCATCCCCACAGAGCCCCTGAGCCACGGC
  AGCACAACTTCATATAAGAAGTCTCTCCGCCTCTCCTCAGACCAGATCGCAAAACTGAAGCTCCACGATG
  GCCCAAATGATGTTGTGTTTAGTATTACAACCCAGTATCAAGGCACCTGTCGCTGTGCAGGGACCATTTA
  CCTGTGGAACTGGAATGACAAGATCATCATTTCTGATATTGATGGGACAATAACCAAGTCGGATGCTTTG
  GGACAGATTCTCCCACAGCTGGGCAAAGACTGGACCCACCAGGGTATAGCAAAGCTCTACCATTCCATCA
  ATGAGAATGGCTACAAGTTTCTGTACTGCTCGGCTCGTGCCATCGGCATGGCCGACATGACCCGTGGCTA
  CCTGCACTGGGTCAATGACAAGGGCACAATCTTGCCCCGGGGCCCCCTGATGCTGTCCCCCAGCAGCTTG
  TTCTCCGCCTTCCACAGAGAAGTGATAGAAAAGAAACCAGAGAAGTTCAAAATTGAGTGTCTAAATGATA
  TCAAGAATCTGTTTGCCCCGTCTAAGCAGCCCTTCTATGCTGCCTTTGGAAACCGTCCAAATGATGTCTA
  TGCCTACACACAAGTTGGAGTTCCAGACTGTAGAATATTCACCGTGAACCCCAAGGGTGAATTAATACAA
  GAAAGAACCAAAGGAAACAAGTCATCGTATCACAGGCTGAGTGAGCTCGTGGAGCATGTGTTCCCCCTTC
  TCAGTAAGGAGCAGAATTCCGCTTTTCCCTGCCCGGAGTTCAGCTCCTTCTGCTACTGGCGAGACCCGAT
  CCCTGAAGTGGACCTGGATGACCTGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224070 representing NM_014646
  Red=Cloning site Green=Tags(s)
  
  MNYVGQLAGQVIVTVKELYKGINQATLSGCIDVIVVQQQDGSYQCSPFHVRFGKLGVLRSKEKVIDIEIN
  GSAVDLHMKLGDNGEAFFVEETEEEYEKLPAYLATSPIPTEDQFFKDIDTPLVKSGGDETPSQSSDISHV
  LETETIFTPSSVKKKKRRRKKYKQDSKKEEQAASAAAEDTCDVGVSSDDDKGAQAARGSSNASLKEEECK
  EPLLFHSGDHYPLSDGDWSPLETTYPQTACPKSDSELEVKPAESLLRSESHMEWTWGGFPESTKVSKRER
  SDHHPRTATITPSENTHFRVIPSEDNLISEVEKDASMEDTVCTIVKPKPRALGTQMSDPTSVAELLEPPL
  ESTQISSMLDADHLPNAALAEAPSESKPAAKVDSPSKKKGVHKRSQHQGPDDIYLDDLKGLEPEVAALYF
  PKSESEPGSRQWPESDTLSGSQSPQSVGSAAADSGTECLSDSAMDLPDVTLSLCGGLSENGEISKEKFME
  HIITYHEFAENPGLIDNPNLVIRIYNRYYNWALAAPMILSLQVFQKSLPKATVESWVKDKMPKKSGRWWF
  WRKRESMTKQLPESKEGKSEAPPASDLPSSSKEPAGARPAENDSSSDEGSQELEESITVDPIPTEPLSHG
  STTSYKKSLRLSSDQIAKLKLHDGPNDVVFSITTQYQGTCRCAGTIYLWNWNDKIIISDIDGTITKSDAL
  GQILPQLGKDWTHQGIAKLYHSINENGYKFLYCSARAIGMADMTRGYLHWVNDKGTILPRGPLMLSPSSL
  FSAFHREVIEKKPEKFKIECLNDIKNLFAPSKQPFYAAFGNRPNDVYAYTQVGVPDCRIFTVNPKGELIQ
  ERTKGNKSSYHRLSELVEHVFPLLSKEQNSAFPCPEFSSFCYWRDPIPEVDLDDLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014646
• ORF Size: 2688 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014646.2, NP_055461.1
• RefSeq Size: 6245 bp
• RefSeq ORF: 2691 bp
• Locus ID: 9663
• UniProt ID: Q92539
• Cytogenetics: 18p11.31
• Domains: lipin_N
• MW: 99.2 kDa
• Gene Summary: Mouse studies suggest that this gene functions during normal adipose tissue development and may play a role in human triglyceride metabolism. This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. [provided by RefSeq, Jul 2008]