STK39 (NM_013233) Human Tagged ORF Clone

CAT#: RC223981

STK39 (Myc-DDK-tagged)-Human serine threonine kinase 39 (STK39)

Product Images

Circular map for RC223981

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK39 (Cat# RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39(Cat# TA500497). Positive lysates LY402227 (100ug) and LC402227 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402227) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223981 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STK39 protein (Cat# TP323981). The protein was produced from HEK293T cells transfected with STK39 cDNA clone (Cat# RC223981) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK39
• Synonyms: DCHT; PASK; SPAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223981 representing NM_013233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCGAGCGGCTCGCCCGTGCACGTCCAGCTTCCCCAGCAGGCGGCCCCGGTGACAGCGGCGG
  CGGCGGCGGCCCCGGCGGCCGCGACAGCAGCGCCGGCCCCGGCAGCTCCCGCGGCCCCGGCCCCGGCCCC
  GGCCCCGGCCCCGGCGGCACAGGCTGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTGCAGGAGGTT
  ATCGGCAGTGGAGCTACTGCTGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGTGTAGCAATAA
  AACGGATCAACTTGGAAAAATGCCAGACCAGTATGGATGAACTATTAAAAGAAATTCAAGCCATGAGTCA
  GTGCAGCCATCCCAACGTAGTGACCTATTACACCTCTTTTGTGGTCAAAGATGAACTTTGGCTGGTCATG
  AAATTACTAAGTGGAGGTTCAATGTTGGATATCATAAAATACATTGTCAACCGAGGAGAACACAAGAATG
  GAGTTCTGGAAGAGGCAATAATAGCAACAATTCTTAAAGAGGTTTTGGAAGGCTTAGACTATCTACACAG
  AAACGGTCAGATTCACAGGGATTTGAAAGCTGGTAATATTCTTCTGGGTGAGGATGGTTCAGTACAAATA
  GCAGATTTTGGGGTAAGTGCGTTCCTAGCAACAGGGGGTGATGTTACCCGAAATAAAGTAAGAAAAACAT
  TCGTTGGCACCCCATGTTGGATGGCTCCTGAAGTCATGGAACAGGTGAGAGGCTATGACTTCAAGGCTGA
  CATGTGGAGTTTTGGAATAACTGCCATTGAATTAGCAACAGGAGCAGCGCCTTATCACAAATATCCTCCC
  ATGAAAGTGTTAATGTTGACTTTGCAAAATGATCCACCCACTTTGGAAACAGGGGTAGAGGATAAAGAAA
  TGATGAAAAAGTACGGCAAGTCCTTTAGAAAATTACTTTCACTGTGTCTTCAGAAAGATCCTTCCAAAAG
  GCCCACAGCAGCAGAACTTTTAAAATGCAAATTCTTCCAGAAAGCCAAGAACAGAGAGTACCTGATTGAG
  AAGCTGCTTACAAGAACACCAGACATAGCCCAAAGAGCCAAAAAGGTAAGAAGAGTTCCTGGGTCAAGTG
  GTCACCTTCATAAAACCGAAGACGGGGACTGGGAGTGGAGTGACGACGAGATGGATGAGAAGAGCGAAGA
  AGGGAAAGCAGCTTTTTCTCAGGAAAAGTCACGAAGAGTAAAAGAAGAAAATCCAGAGATTGCAGTGAGT
  GCCAGCACCATCCCCGAACAAATACAGTCCCTCTCTGTGCACGACTCTCAGGGCCCACCCAATGCTAATG
  AAGACTACAGAGAAGCTTCTTCTTGTGCCGTGAACCTCGTTTTGAGATTAAGAAACTCCAGAAAGGAACT
  TAATGACATACGATTTGAGTTTACTCCAGGAAGAGATACAGCAGATGGTGTATCTCAGGAGCTCTTCTCT
  GCTGGCTTGGTGGATGGTCACGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATGATCCCA
  AAGCTTTAAAAACATTGACATTTAAGTTGGCTTCTGGCTGTGATGGGTCGGAGATTCCTGATGAAGTGAA
  GCTGATTGGGTTTGCTCAGTTGAGTGTCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223981 representing NM_013233
  Red=Cloning site Green=Tags(s)
  
  MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQAVGWPICRDAYELQEV
  IGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVM
  KLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQI
  ADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPP
  MKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIE
  KLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVS
  ASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFS
  AGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013233
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013233.1, NP_037365.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 1638 bp
• Locus ID: 27347
• UniProt ID: Q9UEW8
• Cytogenetics: 2q24.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 59.3 kDa
• Gene Summary: This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008]