XRN2 (NM_012255) Human Tagged ORF Clone

CAT#: RC223980

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XRN2 (Myc-DDK-tagged)-Human 5'-3' exoribonuclease 2 (XRN2)



  "NM_012255" in other vectors (4)

Get anti-DDK tag antibody at $68 with code “DDK”. View details.

USD 851.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Product Name XRN2 (NM_012255) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol XRN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC223980 representing NM_012255
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGTCCCGGCGTTCTTCCGCTGGCTCAGCCGCAAGTACCCGTCCATCATAGTCAACTGCGTGGAAG
AGAAGCCAAAAGAATGCAATGGTGTAAAGATTCCAGTTGATGCCAGTAAACCTAATCCAAATGATGTGGA
GTTTGATAATCTGTATTTGGATATGAATGGAATCATCCATCCCTGTACTCATCCTGAAGACAAACCAGCA
CCAAAAAATGAAGATGAAATGATGGTTGCAATTTTTGAGTACATTGACAGACTTTTCAGTATTGTAAGAC
CAAGAAGACTTCTCTACATGGCAATAGATGGAGTGGCACCACGTGCTAAAATGAACCAGCAGCGTTCAAG
GAGGTTCAGGGCATCAAAAGAAGGAATGGAAGCAGCAGTCGAGAAGCAGCGAGTCAGGGAAGAAATATTG
GCAAAAGGTGGCTTTCTTCCTCCAGAAGAAATAAAAGAAAGATTTGACAGCAACTGTATTACACCAGGAA
CTGAATTCATGGACAATCTTGCTAAATGCCTTCGCTATTACATAGCTGATCGTTTAAATAATGACCCTGG
GTGGAAAAATTTGACAGTTATTTTATCTGATGCTAGTGCTCCTGGTGAAGGAGAACATAAAATCATGGAT
TACATTAGAAGGCAAAGAGCCCAGCCTAACCATGACCCAAATACTCATCATTGTTTATGTGGAGCAGATG
CTGATCTCATTATGCTTGGCCTTGCCACACATGAACCGAACTTTACCATTATTAGAGAAGAATTCAAACC
AAACAAGCCCAAACCATGTGGTCTTTGTAATCAGTTTGGACATGAGGTCAAAGATTGTGAAGGTTTGCCA
AGAGAAAAGAAGGGAAAGCATGATGAACTTGCCGATAGTCTTCCTTGTGCAGAAGGAGAGTTTATCTTCC
TTCGGCTTAATGTTCTTCGTGAGTATTTGGAAAGAGAACTCACAATGGCCAGCCTACCATTCACATTTGA
TGTTGAGAGGAGCATTGATGACTGGGTTTTCATGTGCTTCTTTGTGGGAAATGACTTCCTCCCTCATTTG
CCATCGTTAGAGATTAGGGAAAATGCAATTGACCGTTTGGTTAACATATACAAAAATGTGGTACACAAAA
CTGGGGGTTACCTTACAGAAAGTGGTTATGTCAATCTGCAAAGAGTACAGATGATCATGTTAGCAGTTGG
TGAAGTTGAGGATAGCATTTTTAAAAAGAGAAAGGATGATGAGGACAGTTTTAGAAGACGACAGAAAGAA
AAAAGAAAGAGAATGAAGAGAGATCAACCAGCTTTCACTCCTAGTGGAATATTAACTCCTCATGCCTTGG
GTTCAAGAAATTCACCAGGTTCTCAAGTAGCCAGTAATCCGAGACAAGCAGCCTATGAAATGAGGATGCA
GAATAACTCTAGTCCTTCGATATCTCCTAATACGAGTTTCACATCTGATGGCTCCCCGTCTCCATTAGGA
GGAATTAAGCGAAAAGCAGAAGACAGTGACAGTGAACCTGAGCCAGAGGATAATGTCAGGTTATGGGAAG
CTGGCTGGAAGCAGCGGTACTACAAGAACAAATTTGATGTGGATGCAGCTGATGAGAAATTCCGTCGGAA
AGTTGTGCAGTCGTACGTTGAAGGACTTTGCTGGGTTCTTAGATATTATTACCAGGGCTGTGCTTCCTGG
AAGTGGTATTATCCATTTCATTATGCACCATTTGCTTCAGACTTTGAAGGCATTGCAGACATGCCATCTG
ATTTTGAGAAGGGTACGAAACCGTTTAAACCACTAGAACAACTTATGGGGGTATTTCCAGCTGCAAGTGG
TAATTTTCTACCTCCATCATGGCGGAAGCTCATGAGTGATCCTGATTCTAGTATAATTGACTTCTATCCT
GAAGATTTTGCTATTGATTTGAATGGGAAGAAATATGCATGGCAAGGTGTTGCTCTCTTGCCATTCGTGG
ATGAGCGAAGGCTACGAGCTGCCCTAGAAGAGGTATACCCAGACCTCACTCCAGAAGAGACCAGAAGAAA
CAGCCTTGGAGGTGATGTCTTATTTGTGGGGAAACATCACCCACTCCATGACTTCATTTTAGAGCTGTAC
CAGACAGGTTCCACAGAGCCAGTGGAGGTACCCCCTGAACTATGTCATGGGATTCAAGGAAAGTTTTCTT
TGGATGAAGAAGCCATTCTTCCAGATCAAATAGTATGTTCTCCTGTTCCTATGTTAAGGGATCTGACACA
GAACACTGTAGTCAGTATTAATTTTAAAGACCCACAGTTTGCTGAAGATTACATTTTTAAAGCTGTAATG
CTTCCAGGAGCAAGAAAGCCAGCAGCAGTACTGAAACCTAGTGACTGGGAAAAATCCAGCAATGGACGGC
AGTGGAAGCCTCAGCTTGGCTTTAACCGTGACCGGAGGCCTGTGCACCTGGATCAGGCAGCCTTCAGGAC
TTTGGGCCATGTGATGCCAAGAGGCTCAGGAACTGGCATTTACAGCAATGCTGCACCACCACCTGTGACT
TACCAGGGAAACTTATACAGGCCGCTTTTGAGAGGACAAGCCCAGATTCCAAAACTTATGTCAAATATGA
GGCCCCAGGATTCCTGGCGAGGTCCTCCTCCCCTTTTCCAGCAGCAAAGGTTTGACAGAGGCGTTGGGGC
TGAACCTCTGCTCCCATGGAACCGGATGCTGCAAACCCAGAATGCAGCCTTCCAGCCAAACCAGTACCAG
ATGCTAGCTGGGCCTGGTGGGTATCCACCCAGACGAGATGATCGTGGAGGGAGACAGGGATATCCCAGAG
AAGGAAGGAAATACCCTTTGCCACCACCCTCAGGAAGATACAATTGGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223980 representing NM_012255
Red=Cloning site Green=Tags(s)

MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIHPCTHPEDKPA
PKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEAAVEKQRVREEIL
AKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKNLTVILSDASAPGEGEHKIMD
YIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLP
REKKGKHDELADSLPCAEGEFIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHL
PSLEIRENAIDRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE
KRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPSISPNTSFTSDGSPSPLG
GIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQSYVEGLCWVLRYYYQGCASW
KWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMGVFPAASGNFLPPSWRKLMSDPDSSIIDFYP
EDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELY
QTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVM
LPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVT
YQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNAAFQPNQYQ
MLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012255
ORF Size 2850 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012255.5
RefSeq Size 3449 bp
RefSeq ORF 2853 bp
Locus ID 22803
UniProt ID Q9H0D6
Cytogenetics 20p11.22
Domains DUF251
Protein Pathways RNA degradation
MW 108.4 kDa
Gene Summary This gene encodes a 5'-3' exonuclease that promotes transcription termination at cotranscriptional cleavage sites. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Other Versions

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