ANKS3 (NM_133450) Human Tagged ORF Clone

CAT#: RC223862

ANKS3 (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 3 (ANKS3)

Product Images

Western blot validation of overexpression lysate (Cat# LY408790) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223862 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ANKS3 protein (Cat# TP323862). The protein was produced from HEK293T cells transfected with ANKS3 cDNA clone (Cat# RC223862) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223862 representing NM_133450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGCTCAGCGATGAAGCCAGCGAGCCGGAACTCCTGAACCGCAGCTTGTCCATGTGGCACGGGC
  TCGGGACACAGGTCAGCGGGGAGGAGCTGGATGTCCCCCTGGATCTTCACACAGCTGCTTCCATTGGCCA
  GTATGAAGTGGTGAAGGAGTGTGTGCAGCGGAGAGAGTTAGATTTGAATAAGAAGAATGGTGGTGGCTGG
  ACCCCGCTGATGTATGCCTCCTACATTGGCCACGACACAATCGTGCACCTGCTGCTTGAGGCGGGGGTGA
  GTGTGAATGTGCCGACCCCAGAAGGGCAGACTCCACTGATGCTGGCCTCCAGCTGTGGCAACGAGAGCAT
  CGCCTACTTTCTTCTCCAGCAAGGTGCAGAGCTAGAAATGAAAGACATCCAGGGCTGGACAGCCCTCTTC
  CACTGTACCAGCGCCGGGCACCAGCACATGGTCAGGTTCCTCTTGGACAGTGGAGCCAATGCCAACGTGA
  GGGAGCCGATATGTGGATTTACTCCCTTGATGGAAGCAGCTGCTGCTGGCCATGAGATAATCGTGCAGTA
  TTTTCTGAATCACGGAGTCAAGGTGGACGCGAGAGACCACAGTGGAGCCACAGCCCGGATGCTGGCCAAG
  CAGTACGGACACATGAAGATCGTGGCCTTGATGGACACTTACTCGCCCTCTCTGCCCAAGAGCCTCTATC
  GGAGCCCAGAAAAGTACGAAGATCTGAGCTCTTCTGACGAGTCCTGCCCTGCTCCTCAGAGACAGAGGCC
  TTGCCGGAAGAAGGGTGTCAGCATCCACGAGGGACCGCGAGCCCTGGCCAGGATCACAGGCATTGGCCTG
  GGCGGCAGAGCCCCACGGCCTCGCTATGAGCAGGCTCCTCCCCGTGGCTATGTCACCTTCAACAGCAGTG
  GCGAGAACCCCCTGGAAGAAGAGGGCCTCTGCTGCCGGGATGTCACCTCCCCCATCAATGAGCGGGATGT
  GGAGAGCAGCAGCAGCAGCAGCAGTCGGGAGGAACATGCTTTCTGTGCCAACCTGGGGCCCGTCCAGAGC
  AGCAGCAGCAGCGAGGGCCTGGCCAGAGCCCAGGGGCTCAGCAGCGAAGCTTCTGTGGAGAGCAACGAGG
  ACTCGGATCATGCCTGTAAAAGCTCAGCTCGCAAACAAGCTAAAAGTTACATGAAGACCAAGAATCCTGA
  CAGCCAGTGGCCTCCCCGCGCTGCAACTGACAGGGAAGGCTTTCTCGCTGAGTCCAGCCCCCAGACTCAG
  AGGGCCCCCTACTCAGGACCCCAGGACCTTGCCGCACTGCTGGAGCAGATCGGGTGTCTGAAGTACCTGC
  AGGTGTTTGAGGAGCAGGACGTGGACCTCCGCATCTTTCTGACCCTCACTGAGAGCGACCTGAAGGAAAT
  TGGCATCACGCTGTTTGGGCCCAAGAGGAAGATGACGTCCGCCATTGCCCGCTGGCACAGCAGTGCCCGC
  CCACCCGGGGATGCCCTGGAGCTGGCCTACGCCGACCGGCTGGAGGCTGAGATGCAGGAGCTCGCCATCC
  AGCTGCACAAGCGCTGCGAGGAGGTAGAGGCCACGCGGGGCCAGGTGTGTCAGGAGCAGGAGCTGCGCGC
  CGTGGTGGAGAGCTGCCTGCTGGAGCAGGACCGCGCCCGCGAGGACCTCCAGGCCCGGCTGCGGGAGACG
  TGGGCCCTGGCCCGGGATGCTGCCCTCGTCCTGGACCAGCTGCGAGCCTGTCAAGCTGAGCTGTCATCTC
  GAGTGAGGCAGGACCAGCCCCCTGGTGCAGCCACTCTGGGCCTAGCCGTCCCCCCAGCTGACTCCAAGGG
  CTGGCAAGCGTCCCTGCAGGCCATGAGCCTCCCCGAGCTCTCGGGAGCCCTGGAGGACCGTGTCCGTGAG
  ATGGGGCAAGCACTGTGCTTAGTGACCCAGAGCCTGGAGAAGCTGCAGGTGCTGAACGGGAAGAAGTGGC
  GGGAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223862 representing NM_133450
  Red=Cloning site Green=Tags(s)
  
  MSELSDEASEPELLNRSLSMWHGLGTQVSGEELDVPLDLHTAASIGQYEVVKECVQRRELDLNKKNGGGW
  TPLMYASYIGHDTIVHLLLEAGVSVNVPTPEGQTPLMLASSCGNESIAYFLLQQGAELEMKDIQGWTALF
  HCTSAGHQHMVRFLLDSGANANVREPICGFTPLMEAAAAGHEIIVQYFLNHGVKVDARDHSGATARMLAK
  QYGHMKIVALMDTYSPSLPKSLYRSPEKYEDLSSSDESCPAPQRQRPCRKKGVSIHEGPRALARITGIGL
  GGRAPRPRYEQAPPRGYVTFNSSGENPLEEEGLCCRDVTSPINERDVESSSSSSSREEHAFCANLGPVQS
  SSSSEGLARAQGLSSEASVESNEDSDHACKSSARKQAKSYMKTKNPDSQWPPRAATDREGFLAESSPQTQ
  RAPYSGPQDLAALLEQIGCLKYLQVFEEQDVDLRIFLTLTESDLKEIGITLFGPKRKMTSAIARWHSSAR
  PPGDALELAYADRLEAEMQELAIQLHKRCEEVEATRGQVCQEQELRAVVESCLLEQDRAREDLQARLRET
  WALARDAALVLDQLRACQAELSSRVRQDQPPGAATLGLAVPPADSKGWQASLQAMSLPELSGALEDRVRE
  MGQALCLVTQSLEKLQVLNGKKWRET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133450
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133450.4
• RefSeq Size: 2663 bp
• RefSeq ORF: 1971 bp
• Locus ID: 124401
• UniProt ID: Q6ZW76
• Cytogenetics: 16p13.3
• MW: 71.9 kDa
• Gene Summary: May be involved in vasopressin signaling in the kidney.[UniProtKB/Swiss-Prot Function]