IL31 (NM_001014336) Human Tagged ORF Clone

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SKU
RC223858
IL31 (Myc-DDK-tagged)-Human interleukin 31 (IL31)
  $150.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IL31
Synonyms IL-31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223858 representing NM_001014336
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTCACTCAGGCCCCTCGACGTCTGTGCTCTTTCTGTTCTGCTGCCTGGGAGGCTGGCTGGCCT
CCCACACGTTGCCCGTCCGTTTACTACGACCAAGTGATGATGTACAGAAAATAGTCGAGGAATTACAGTC
CCTCTCGAAGATGCTTTTGAAAGATGTGGAGGAAGAGAAGGGCGTGCTCGTGTCCCAGAATTACACGCTG
CCGTGTCTCAGCCCTGACGCCCAGCCGCCAAACAACATCCACAGCCCAGCCATCCGGGCATATCTCAAGA
CAATCAGACAGCTAGACAACAAATCTGTTATTGATGAGATCATAGAGCACCTCGACAAACTCATATTTCA
AGATGCACCAGAAACAAACATTTCTGTGCCAACAGACACCCATGAATGTAAACGCTTCATCCTGACTATT
TCTCAACAGTTTTCAGAGTGCATGGACCTCGCACTAAAATCATTGACCTCTGGAGCCCAACAGGCCACCA
CT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC223858 representing NM_001014336
Red=Cloning site Green=Tags(s)

MASHSGPSTSVLFLFCCLGGWLASHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTL
PCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTI
SQQFSECMDLALKSLTSGAQQATT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001014336
ORF Size 492 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001014336.2
RefSeq Size 904 bp
RefSeq ORF 495 bp
Locus ID 386653
UniProt ID Q6EBC2
Cytogenetics 12q24.31
Protein Families Secreted Protein, Transmembrane
MW 18 kDa
Summary IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 PubMed 15184896).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_001014336" in other vectors (6)
SKU Description Size Price
RC223858L1 Lenti ORF clone of Human interleukin 31 (IL31), Myc-DDK-tagged 10 ug
$450.00
RC223858L2 Lenti ORF clone of Human interleukin 31 (IL31), mGFP tagged 10 ug
$450.00
RC223858L3 Lenti ORF clone of Human interleukin 31 (IL31), Myc-DDK-tagged 10 ug
$450.00
RC223858L4 Lenti ORF clone of Human interleukin 31 (IL31), mGFP tagged 10 ug
$450.00
RG223858 IL31 (tGFP-tagged) - Human interleukin 31 (IL31) 10 ug
$489.00
SC301922 IL31 (untagged)-Human interleukin 31 (IL31) 10 ug
$150.00

Plasmid Map for RC223858

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Cloning scheme for RC223858

Circular map for RC223858

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