BTN1A1 (NM_001732) Human Tagged ORF Clone

CAT#: RC223852

BTN1A1 (Myc-DDK-tagged)-Human butyrophilin, subfamily 1, member A1 (BTN1A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTN1A1 (Cat# RC223852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1(Cat# TA501529). Positive lysates LY400653 (100ug) and LC400653 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400653) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223852 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BTN1A1 protein (Cat# TP323852). The protein was produced from HEK293T cells transfected with BTN1A1 cDNA clone (Cat# RC223852) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BTN1A1
• Synonyms: BT; BTN; BTN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223852 representing NM_001732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTTTCCCAAGCTCCGGTCTCCCCAGATGTCTGCTCACCCTCATTCTCCTCCAGCTGCCCAAAC
  TGGATTCAGCTCCCTTTGACGTGATTGGACCCCCGGAGCCCATCCTGGCCGTTGTGGGTGAGGACGCCGA
  GCTGCCCTGTCGCCTGTCCCCGAACGCGAGCGCCGAGCACTTGGAGCTACGCTGGTTCCGAAAGAAGGTT
  TCGCCGGCCGTGCTGGTGCATAGGGACGGGCGCGAGCAGGAAGCCGAGCAGATGCCCGAGTACCGCGGGC
  GGGCGACGCTGGTCCAGGACGGCATCGCCAAGGGGCGCGTGGCCTTGAGGATCCGTGGCGTCAGAGTCTC
  TGACGACGGGGAGTACACGTGCTTTTTCAGGGAGGATGGAAGCTACGAAGAAGCCCTGGTGCATCTGAAG
  GTGGCTGCTCTGGGCTCTGACCCTCACATCAGTATGCAAGTTCAAGAGAATGGAGAAATCTGTCTGGAGT
  GCACCTCAGTGGGATGGTACCCAGAGCCCCAGGTGCAGTGGAGAACTTCCAAGGGAGAGAAGTTTCCATC
  TACATCAGAGTCCAGGAATCCTGATGAAGAAGGTTTGTTCACTGTGGCTGCTTCAGTGATCATCAGAGAC
  ACTTCTACGAAAAATGTGTCCTGCTACATCCAGAATCTCCTTCTTGGACAGGAGAAGAAAGTAGAAATAT
  CCATACCAGCTTCCTCCCTCCCAAGGCTGACTCCCTGGATAGTGGCTGTGGCTGTCATCCTGATGGTTCT
  AGGACTTCTCACCATTGGGTCCATATTTTTCACTTGGAGACTATACAACGAAAGACCCAGAGAGAGGAGG
  AATGAATTCAGCTCTAAAGAGAGACTCCTGGAAGAACTCAAATGGAAAAAGGCTACCTTGCATGCAGTTG
  ATGTGACTCTGGACCCAGACACAGCTCATCCCCACCTCTTTCTTTATGAGGATTCAAAATCTGTTCGACT
  GGAAGATTCACGTCAGAAACTGCCTGAGAAAACAGAGAGATTTGACTCCTGGCCCTGTGTGTTGGGCCGT
  GAGACCTTCACCTCAGGAAGGCATTACTGGGAGGTGGAGGTGGGAGACAGGACTGACTGGGCAATCGGCG
  TGTGTAGGGAGAATGTGATGAAGAAAGGATTTGACCCCATGACTCCTGAGAATGGGTTCTGGGCTGTAGA
  GTTGTATGGAAATGGGTACTGGGCCCTCACTCCTCTCCGGACCCCTCTCCCATTGGCAGGGCCCCCACGC
  CGGGTTGGGATTTTCCTAGACTATGAATCAGGAGACATCTCCTTCTACAACATGAATGATGGATCTGATA
  TCTATACTTTCTCCAATGTCACTTTCTCTGGCCCCCTCCGGCCCTTCTTTTGCCTATGGTCTAGCGGTAA
  AAAGCCCCTGACCATCTGCCCAATTGCTGATGGGCCTGAGAGGGTCACAGTCATTGCTAATGCCCAGGAC
  CTTTCTAAGGAGATCCCATTGTCCCCCATGGGGGAGGAGTCTGCCCCTAGGGATGCAGACACTCTCCATT
  CTAAGCTAATCCCTACCCAACCCAGCCAAGGGGCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223852 representing NM_001732
  Red=Cloning site Green=Tags(s)
  
  MAVFPSSGLPRCLLTLILLQLPKLDSAPFDVIGPPEPILAVVGEDAELPCRLSPNASAEHLELRWFRKKV
  SPAVLVHRDGREQEAEQMPEYRGRATLVQDGIAKGRVALRIRGVRVSDDGEYTCFFREDGSYEEALVHLK
  VAALGSDPHISMQVQENGEICLECTSVGWYPEPQVQWRTSKGEKFPSTSESRNPDEEGLFTVAASVIIRD
  TSTKNVSCYIQNLLLGQEKKVEISIPASSLPRLTPWIVAVAVILMVLGLLTIGSIFFTWRLYNERPRERR
  NEFSSKERLLEELKWKKATLHAVDVTLDPDTAHPHLFLYEDSKSVRLEDSRQKLPEKTERFDSWPCVLGR
  ETFTSGRHYWEVEVGDRTDWAIGVCRENVMKKGFDPMTPENGFWAVELYGNGYWALTPLRTPLPLAGPPR
  RVGIFLDYESGDISFYNMNDGSDIYTFSNVTFSGPLRPFFCLWSSGKKPLTICPIADGPERVTVIANAQD
  LSKEIPLSPMGEESAPRDADTLHSKLIPTQPSQGAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001732
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001732.1, NP_001723.1
• RefSeq Size: 2901 bp
• RefSeq ORF: 1581 bp
• Locus ID: 696
• UniProt ID: Q13410
• Cytogenetics: 6p22.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 58.8 kDa
• Gene Summary: Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq, Jul 2008]