FBXW7 (NM_018315) Human Tagged ORF Clone

CAT#: RC223843

FBXW7 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 2

Product Images

Circular map for RC223843

• 

  Western blot validation of overexpression lysate (Cat# LY413158) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223843 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXW7
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223843 representing NM_018315
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTCCCGAGAAGCGGTTTGATACTGAGCTGCATTTGCCTTTACTGTGGAGTTTTGTTGCCGGTTC
  TGCTCCCTAATCTTCCTTTTCTGACGTGCCTGAGCATGTCCACATTAGAATCTGTGACATACCTACCTGA
  AAAAGGTTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCACAGAATCACTGAAGGGGAAA
  AATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGATTTTTTACAAAATGAAAAGAAAGTTGGACC
  ATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCAC
  TGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA
  CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATGTTTCAGAGCT
  GGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGTGAACCAACACAAGTAAAACA
  TATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTTCATTGCTCCCTAAAGAGTTGGCACTC
  TATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTT
  TGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAA
  GAGAAGAAAAGTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA
  ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGATGATCATGTGA
  TCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTC
  AGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGA
  GACAACATCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTA
  TACACACCTTATATGGGCATACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGG
  TTCTCGAGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT
  GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGATTTTATGGTAA
  AGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCATACTAATAGAGTCTATTCATT
  ACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGTGTTTGGGATGTGGAGACA
  GGGAATTGCATTCACACGTTAACAGGGCACCAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTC
  TTGTCTCTGGGAATGCAGATTCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATT
  GCAAGGTCCCAACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC
  TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTAGTCACATTGG
  AGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACAAAGCTGGTGTGTGCAGTTGG
  GAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTGGACTTTGATGTGGACATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223843 representing NM_018315
  Red=Cloning site Green=Tags(s)
  
  MCVPRSGLILSCICLYCGVLLPVLLPNLPFLTCLSMSTLESVTYLPEKGLYCQRLPSSRTHGGTESLKGK
  NTENMGFYGTLKMIFYKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQ
  QRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELAL
  YVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHR
  IDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMR
  DNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGH
  VAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVET
  GNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITS
  SDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018315
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018315.5
• RefSeq Size: 3603 bp
• RefSeq ORF: 1884 bp
• Locus ID: 55294
• UniProt ID: Q969H0
• Cytogenetics: 4q31.3
• Domains: WD40, F-box
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 70.1 kDa
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]