FBXW7 (NM_018315) Human Tagged ORF Clone

CAT#: RC223843

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FBXW7 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 2



  "NM_018315" in other vectors (6)

Reconstitution Protocol

USD 584.00

USD 875.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FBXW7
Synonyms AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223843 representing NM_018315
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGTCCCGAGAAGCGGTTTGATACTGAGCTGCATTTGCCTTTACTGTGGAGTTTTGTTGCCGGTTC
TGCTCCCTAATCTTCCTTTTCTGACGTGCCTGAGCATGTCCACATTAGAATCTGTGACATACCTACCTGA
AAAAGGTTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCACAGAATCACTGAAGGGGAAA
AATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGATTTTTTACAAAATGAAAAGAAAGTTGGACC
ATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCAC
TGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA
CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATGTTTCAGAGCT
GGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGTGAACCAACACAAGTAAAACA
TATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTTCATTGCTCCCTAAAGAGTTGGCACTC
TATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTT
TGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAA
GAGAAGAAAAGTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA
ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGATGATCATGTGA
TCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTC
AGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGA
GACAACATCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTA
TACACACCTTATATGGGCATACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGG
TTCTCGAGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT
GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGATTTTATGGTAA
AGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCATACTAATAGAGTCTATTCATT
ACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGTGTTTGGGATGTGGAGACA
GGGAATTGCATTCACACGTTAACAGGGCACCAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTC
TTGTCTCTGGGAATGCAGATTCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATT
GCAAGGTCCCAACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC
TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTAGTCACATTGG
AGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACAAAGCTGGTGTGTGCAGTTGG
GAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTGGACTTTGATGTGGACATGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223843 representing NM_018315
Red=Cloning site Green=Tags(s)

MCVPRSGLILSCICLYCGVLLPVLLPNLPFLTCLSMSTLESVTYLPEKGLYCQRLPSSRTHGGTESLKGK
NTENMGFYGTLKMIFYKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQ
QRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELAL
YVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHR
IDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMR
DNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGH
VAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVET
GNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITS
SDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018315
ORF Size 1881 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018315.5
RefSeq Size 3603 bp
RefSeq ORF 1884 bp
Locus ID 55294
UniProt ID Q969H0
Cytogenetics 4q31.3
Domains WD40, F-box
Protein Families Druggable Genome, Transmembrane
Protein Pathways Ubiquitin mediated proteolysis
MW 70.1 kDa
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

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