IQGAP2 (NM_006633) Human Tagged ORF Clone

CAT#: RC223783

IQGAP2 (Myc-DDK-tagged)-Human IQ motif containing GTPase activating protein 2 (IQGAP2)

Product Images

Circular map for RC223783

• 

  Western blot validation of overexpression lysate (Cat# LY416521) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223783 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IQGAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223783 representing NM_006633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCT
  CTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGC
  CAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGG
  AATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATG
  ATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTT
  AAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAA
  AACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCC
  AGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGA
  GAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGAT
  GAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCG
  TTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAA
  AGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAA
  GAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGC
  AGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAA
  GAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAAC
  CTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTG
  CTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCC
  CGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAA
  CTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTA
  ATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGG
  GAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCAT
  GCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCA
  AAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACA
  ATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTA
  TTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTG
  TGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAA
  AAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTAT
  AAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTG
  AGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAG
  GGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTT
  TGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTG
  TTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTT
  CAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTAC
  AAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAA
  GTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGC
  CAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATC
  ACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGA
  ATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCA
  GCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAG
  TCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATC
  TACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACAT
  AGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACC
  CTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACC
  CTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTA
  TGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAAC
  CTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGA
  GGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATT
  AAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGAT
  ATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGG
  TTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTA
  TTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAG
  AAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAG
  AAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACT
  GAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGAC
  CCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAA
  GCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGAT
  AATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAA
  CAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGA
  AGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAA
  TCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATT
  GCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGA
  CCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAA
  GACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCC
  AAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAG
  ATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGG
  CATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTA
  CTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTC
  TCATATACCTGCTGAACAAGAAGTTCTATGGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223783 representing NM_006633
  Red=Cloning site Green=Tags(s)
  
  MPHEELPSLQRPRYGSIVDDERLSAEEMDERRRQNIAYEYLCHLEEAKRWMEVCLVEELPPTTELEEGLR
  NGVYLAKLAKFFAPKMVSEKKIYDVEQTRYKKSGLHFRHTDNTVQWLRAMESIGLPKIFYPETTDVYDRK
  NIPRMIYCIHALSLYLFKLGIAPQIQDLLGKVDFTEEEISNMRKELEKYGIQMPSFSKIGGILANELSVD
  EAALHAAVIAINEAVEKGIAEQTVVTLRNPNAVLTLVDDNLAPEYQKELWDAKKKKEENARLKNSCISEE
  ERDAYEELLTQAEIQGNINKVNRQAAVDHINAVIPEGDPENTLLALKKPEAQLPAVYPFAAAMYQNELFN
  LQKQNTMNYLAHEELLIAVEMLSAVALLNQALESNDLVSVQNQLRSPAIGLNNLDKAYVERYANTLLSVK
  LEVLSQGQDNLSWNEIQNCIDMVNAQIQEENDRVVAVGYINEAIDEGNPLRTLETLLLPTANISDVDPAH
  AQHYQDVLYHAKSQKLGDSESVSKVLWLDEIQQAVDEANVDEDRAKQWVTLVVDVNQCLEGKKSSDILSV
  LKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSWLKLNLHKKYDYYYNTDSKESSWVTPESCFY
  KESWLTGKEIEDIIEEVTVGYIRENIWSASEELLLRFQATSSGPILREEFEARKSFLHEQEENVVKIQAF
  WKGYKQRKEYMHRRQTFIDNTDSVVKIQSWFRMATARKSYLSRLQYFRDHNNEIVKIQSLLRANKARDDY
  KTLVGSENPPLTVIRKFVYLLDQSDLDFQEELEVARLREEVVTKIRANQQLEKDLNLMDIKIGLLVKNRI
  TLEDVISHSKKLNKKKGGEMEILNNTDNQGIKSLSKERRKTLETYQQLFYLLQTNPLYLAKLIFQMPQNK
  STKFMDTVIFTLYNYASNQREEYLLLKLFKTALEEEIKSKVDQVQDIVTGNPTVIKMVVSFNRGARGQNT
  LRQLLAPVVKEIIDDKSLIINTNPVEVYKAWVNQLETQTGEASKLPYDVTTEQALTYPEVKNKLEASIEN
  LRRVTDKVLNSIISSLDLLPYGLRYIAKVLKNSIHEKFPDATEDELLKIVGNLLYYRYMNPAIVAPDGFD
  IIDMTAGGQINSDQRRNLGSVAKVLQHAASNKLFEGENEHLSSMNNYLSETYQEFRKYFKEACNVPEPEE
  KFNMDKYTDLVTVSKPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSELLGSLGEVPTVESFLGEGAVD
  PNDPNKANTLSQLSKTEISLVLTSKYDIEDGEAIDSRSLMIKTKKLIIDVIRNQPGNTLTEILETPATAQ
  QEVDHATDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRTLEQTGHVSSENKYQDILNEI
  AKDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQINYYDTYIKTCLDNLKRKNTRRSIKLDGKGEP
  KGAKRAKPVKYTAAKLHEKGVLLDIDDLQTNQFKNVTFDIIATEDVGIFDVRSKFLGVEMEKVQLNIQDL
  LQMQYEGVAVMKMFDKVKVNVNLLIYLLNKKFYGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006633
• ORF Size: 4725 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006633.1, NP_006624.1
• RefSeq Size: 5767 bp
• RefSeq ORF: 4728 bp
• Locus ID: 10788
• UniProt ID: Q13576
• Cytogenetics: 5q13.3
• Domains: IQ, RasGAP_C, CH, RasGAP
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 180.4 kDa
• Gene Summary: This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017]