HEPACAM (NM_152722) Human Tagged ORF Clone

CAT#: RC223693

HEPACAM (Myc-DDK-tagged)-Human hepatic and glial cell adhesion molecule (HEPACAM)

Product Images

Circular map for RC223693

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  Western blot validation of overexpression lysate (Cat# LY407342) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223693 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HEPACAM protein (Cat# TP323693). The protein was produced from HEK293T cells transfected with HEPACAM cDNA clone (Cat# RC223693) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HEPACAM
• Synonyms: GlialCAM; HEPN1; MLC2A; MLC2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223693 representing NM_152722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGAGAAAGGGGAGCCCTGTCCAGAGCCTCCAGGGCCCTGCGCCTTGCTCCTTTTGTCTACCTTC
  TTCTGATCCAGACAGACCCCCTGGAGGGGGTGAACATCACCAGCCCCGTGCGCCTGATCCATGGCACCGT
  GGGGAAGTCGGCTCTGCTTTCTGTGCAGTACAGCAGTACCAGCAGCGACAGGCCTGTAGTGAAGTGGCAG
  CTGAAGCGGGACAAGCCAGTGACCGTGGTGCAGTCCATTGGCACAGAGGTCATCGGCACCCTGCGGCCTG
  ACTATCGAGACCGTATCCGACTCTTTGAAAATGGCTCCCTGCTTCTCAGCGACCTGCAGCTGGCCGATGA
  GGGCACCTATGAGGTCGAGATCTCCATCACCGACGACACCTTCACTGGGGAGAAGACCATCAACCTTACT
  GTAGATGTGCCCATTTCGAGGCCACAGGTGTTGGTGGCTTCAACCACTGTGCTGGAGCTCAGCGAGGCCT
  TCACCTTGAACTGCTCACATGAGAATGGCACCAAGCCCAGCTACACCTGGCTGAAGGATGGCAAGCCCCT
  CCTCAATGACTCGAGAATGCTCCTGTCCCCCGACCAAAAGGTGCTCACCATCACCCGCGTGCTCATGGAG
  GATGACGACCTGTACAGCTGCGTGGTGGAGAACCCCATCAGCCAGGGCCGCAGCCTGCCTGTCAAGATCA
  CCGTATACAGAAGAAGCTCCCTTTACATCATCTTGTCTACAGGAGGCATCTTCCTCCTTGTGACCTTGGT
  GACAGTCTGTGCCTGCTGGAAACCCTCCAAAAGGAAACAGAAGAAGCTAGAAAAGCAAAACTCCCTGGAA
  TACATGGATCAGAATGATGACCGCCTGAAACCAGAAGCAGACACCCTCCCTCGAAGTGGTGAGCAGGAAC
  GGAAGAACCCCATGGCACTCTATATCCTGAAGGACAAGGACTCCCCGGAGACCGAGGAGAACCCGGCCCC
  GGAGCCTCGAAGCGCGACGGAGCCCGGCCCGCCCGGCTACTCCGTGTCTCCCGCCGTGCCCGGCCGCTCG
  CCGGGGCTGCCCATCCGCTCTGCCCGCCGCTACCCGCGCTCCCCAGCGCGCTCCCCAGCCACCGGCCGGA
  CACACTCGTCGCCGCCCAGGGCCCCGAGCTCGCCCGGCCGCTCGCGCAGCGCCTCGCGCACACTGCGGAC
  TGCGGGCGTGCACATAATCCGCGAGCAAGACGAGGCCGGCCCGGTGGAGATCAGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223693 representing NM_152722
  Red=Cloning site Green=Tags(s)
  
  MKRERGALSRASRALRLAPFVYLLLIQTDPLEGVNITSPVRLIHGTVGKSALLSVQYSSTSSDRPVVKWQ
  LKRDKPVTVVQSIGTEVIGTLRPDYRDRIRLFENGSLLLSDLQLADEGTYEVEISITDDTFTGEKTINLT
  VDVPISRPQVLVASTTVLELSEAFTLNCSHENGTKPSYTWLKDGKPLLNDSRMLLSPDQKVLTITRVLME
  DDDLYSCVVENPISQGRSLPVKITVYRRSSLYIILSTGGIFLLVTLVTVCACWKPSKRKQKKLEKQNSLE
  YMDQNDDRLKPEADTLPRSGEQERKNPMALYILKDKDSPETEENPAPEPRSATEPGPPGYSVSPAVPGRS
  PGLPIRSARRYPRSPARSPATGRTHSSPPRAPSSPGRSRSASRTLRTAGVHIIREQDEAGPVEISA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152722
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152722.3, NP_689935.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 1251 bp
• Locus ID: 220296
• UniProt ID: Q14CZ8
• Cytogenetics: 11q24.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 45.8 kDa
• Gene Summary: The protein encoded by this gene is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. The encoded protein acts as a homodimer and is involved in cell motility and cell-matrix interactions. The expression of this gene is downregulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene. [provided by RefSeq, Jul 2011]