KCC2 (SLC12A5) (NM_020708) Human Tagged ORF Clone

CAT#: RC223680

SLC12A5 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2

Product Images

Circular map for RC223680

• 

  Western blot validation of overexpression lysate (Cat# LY402800) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223680 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLC12A5 protein (Cat# TP323680). The protein was produced from HEK293T cells transfected with SLC12A5 cDNA clone (Cat# RC223680) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCC2
• Synonyms: DEE34; EIEE34; EIG14; hKCC2; KCC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223680 representing NM_020708
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCGGGTGATGGCAACCCCAAGG
  AAAGCAGTCCCTTCATCAACAGCACCGACACAGAGAAGGGAAAGGAGTATGATGGCAAGAACATGGCCTT
  GTTTGAGGAGGAGATGGACACCAGCCCTATGGTGTCCTCCTTGCTCAGTGGCCTGGCCAACTACACCAAC
  CTGCCCCAGGGAAGTAGGGAGCATGAAGAGGCAGAAAACAATGAGGGTGGAAAAAAGAAGCCGGTGCAGG
  CCCCACGCATGGGCACCTTCATGGGCGTGTACCTGCCGTGCCTGCAGAACATCTTTGGCGTCATCCTCTT
  CCTGCGGCTCACCTGGGTGGTGGGCATTGCAGGCATCATGGAGTCCTTCTGCATGGTGTTCATCTGCTGC
  TCCTGTACGATGCTCACGGCCATCTCCATGAGTGCAATTGCAACGAATGGTGTTGTGCCTGCTGGTGGCT
  CCTACTACATGATTTCCAGGTCTCTGGGCCCAGAGTTTGGGGGTGCCGTGGGCCTCTGCTTCTACCTGGG
  CACTACCTTTGCAGGAGCCATGTACATCCTGGGCACCATCGAAATCCTGCTGGCTTACCTCTTCCCAGCC
  ATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGAGGCAGCAGCCATGCTGAACAACATGCGTGTTTACG
  GCACCTGTGTGCTCACCTGCATGGCCACTGTGGTGTTTGTGGGTGTCAAGTATGTCAACAAGTTTGCCCT
  TGTCTTCCTGGGTTGTGTCATCCTCTCCATCCTGGCCATCTATGCTGGGGTCATCAAGTCTGCCTTCGAC
  CCACCCAACTTCCCGATCTGCCTCCTGGGTAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTGTGCCA
  AGCTGGCTTGGGAAGGAAATGAGACGGTGACCACACGGCTATGGGGCCTTTTCTGCTCCTCTCGCTTCCT
  CAACGCCACCTGTGATGAATACTTCACCCGAAACAATGTCACAGAGATCCAGGGCATCCCTGGTGCTGCC
  AGTGGCCTCATCAAAGAGAACCTCTGGAGCTCCTACCTGACCAAGGGCGTGATTGTGGAGAGGAGTGGGA
  TGACCTCGGTGGGCCTGGCCGATGGCACTCCTATCGACATGGACCACCCTTATGTCTTCAGTGATATGAC
  CTCCTACTTCACCCTGCTGGTTGGCATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGTTCTAACCGC
  TCTGGGGACCTGAGGGATGCCCAGAAGTCAATCCCCACTGGCACCATCCTGGCCATCGCCACCACCTCTG
  CTGTCTACATCAGCTCCGTTGTTCTGTTTGGGGCCTGCATTGAGGGGGTCGTCCTGCGGGACAAGTTTGG
  CGAAGCTGTGAATGGCAACCTCGTGGTGGGCACTCTGGCCTGGCCATCTCCATGGGTAATTGTCATCGGA
  TCCTTCTTCTCCACCTGTGGGGCTGGGCTGCAGAGCCTCACGGGGGCCCCACGCCTGCTGCAGGCCATCT
  CGAGGGATGGCATTGTGCCCTTCCTGCAGGTCTTTGGCCATGGCAAGGCCAATGGAGAGCCGACCTGGGC
  CCTGCTCCTGACTGCCTGCATCTGCGAGATTGGCATCCTCATTGCATCCCTCGACGAGGTGGCCCCCATC
  CTCTCTATGTTCTTCCTGATGTGCTACATGTTTGTGAATCTGGCCTGTGCAGTGCAGACGCTGCTGAGGA
  CACCCAACTGGAGGCCACGCTTTCGATATTACCACTGGACCCTCTCCTTCCTGGGCATGAGCCTCTGCCT
  GGCCCTCATGTTCATCTGCTCCTGGTATTATGCACTGGTAGCCATGCTCATTGCTGGACTCATCTACAAG
  TACATTGAGTACCGTGGGGCAGAGAAGGAGTGGGGCGATGGGATACGAGGTCTGTCTCTCAGTGCGGCTC
  GCTATGCCCTCTTACGCCTGGAGGAAGGGCCCCCACACACCAAGAACTGGAGGCCACAGCTGCTGGTGCT
  GGTGCGTGTGGACCAAGACCAGAATGTGGTGCACCCCCAGCTGCTCTCACTGACCTCCCAGCTGAAGGCA
  GGGAAGGGCCTGACCATCGTGGGCTCTGTCCTTGAGGGCACCTTTCTGGAAAATCATCCACAGGCCCAGC
  GGGCAGAAGAGTCTATCAGGCGCCTGATGGAGGCAGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGATCTC
  CTCCAACTTGCGTGATGGCGTGTCCCATCTGATCCAGTCCGGGGGCCTCGGGGGGCTGCAGCACAACACT
  GTGCTTGTTGGCTGGCCCCGCAACTGGCGCCAGAAGGAAGATCATCAGACATGGAGGAACTTCATTGAGC
  TGGTCCGGGAAACCACAGCTGGCCACTTAGCCCTGCTGGTCACCAAGAACGTTTCCATGTTTCCTGGGAA
  CCCTGAGCGCTTCTCTGAGGGCAGCATCGACGTTTGGTGGATTGTGCACGATGGAGGCATGCTCATGCTG
  CTGCCCTTCCTGCTGCGGCACCACAAGGTCTGGCGGAAGTGCAAGATGCGTATCTTCACTGTGGCCCAGA
  TGGATGACAATAGCATCCAGATGAAGAAGGATCTGACCACATTTCTGTATCATTTACGCATCACTGCGGA
  GGTCGAGGTGGTGGAGATGCATGAGAGCGACATCTCAGCTTACACCTATGAGAAGACGTTGGTGATGGAG
  CAGCGTTCCCAGATCCTCAAACAGATGCATTTAACCAAGAATGAGCGGGAGCGGGAGATCCAGAGTATCA
  CAGATGAGTCACGAGGCTCAATCCGGAGAAAGAATCCAGCCAACACGCGGCTCCGCCTGAACGTCCCAGA
  AGAGACGGCTGGTGACAGTGAAGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCACGATCAGAGTGCTCCC
  AGCTGCCCCAGCAGCTCCCCGTCCCCAGGGGAGGAGCCTGAGGGGGAAGGGGAGACAGATCCGGAGAAGG
  TGCATCTCACCTGGACCAAGGACAAGTCGGTGGCAGAGAAGAATAAGGGCCCCAGTCCTGTCTCCTCTGA
  GGGCATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAGAACTTGAACCAGTCCAACGTGCGGCGCATG
  CACACGGCCGTGCGGCTGAACGAGGTCATCGTGAAGAAATCCCGGGACGCCAAGCTTGTTTTGCTCAACA
  TGCCTGGGCCTCCCCGCAACCGCAATGGTGATGAAAACTACATGGAGTTTCTCGAGGTCCTCACAGAGCA
  CCTGGACCGGGTGATGCTGGTCCGCGGCGGCGGCCGCGAGGTCATCACCATCTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223680 representing NM_020708
  Red=Cloning site Green=Tags(s)
  
  MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTN
  LPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICC
  SCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPA
  MAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFD
  PPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAA
  SGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR
  SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIG
  SFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPI
  LSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYK
  YIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKA
  GKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNT
  VLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLML
  LPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVME
  QRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAP
  SCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRM
  HTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020708
• ORF Size: 3348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020708.5
• RefSeq Size: 6059 bp
• RefSeq ORF: 3351 bp
• Locus ID: 57468
• UniProt ID: Q9H2X9
• Cytogenetics: 20q13.12
• Protein Families: Transmembrane
• MW: 123.3 kDa
• Gene Summary: K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]