GGT1 (NM_013430) Human Tagged ORF Clone

CAT#: RC223640

GGT1 (Myc-DDK-tagged)-Human gamma-glutamyltransferase 1 (GGT1), transcript variant 3

Product Images

Circular map for RC223640

• 

  Western blot validation of overexpression lysate (Cat# LY415599) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223640 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGT1
• Synonyms: CD224; D22S672; D22S732; GGT; GGT 1; GGTD; GTG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223640 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGAAGTTAGTGGTGCTGGGCCTGCTGGCCGTGGTCCTGGTGCTGGTCATTGTCGGCCTCTGTC
  TCTGGCTGCCCTCAGCCTCCAAGGAACCTGACAACCATGTGTACACCAGGGCTGCCGTGGCCGCGGATGC
  CAAGCAGTGCTCGAAGATTGGGAGGGATGCACTGCGGGACGGTGGCTCTGCGGTGGATGCAGCCATTGCA
  GCCCTGTTGTGTGTGGGGCTCATGAATGCCCACAGCATGGGCATCGGGGGTGGCCTCTTCCTCACCATCT
  ACAACAGCACCACACGAAAAGCTGAGGTCATCAACGCCCGCGAGGTGGCCCCCAGGCTGGCCTTTGCCAC
  CATGTTCAACAGCTCGGAGCAGTCCCAGAAGGGGGGGCTGTCGGTGGCGGTGCCTGGGGAGATCCGAGGC
  TATGAGCTGGCACACCAGCGGCATGGGCGGCTGCCCTGGGCTCGCCTCTTCCAGCCCAGCATCCAGCTGG
  CCCGCCAGGGCTTCCCCGTGGGCAAGGGCTTGGCGGCAGCCCTGGAAAACAAGCGGACCGTCATCGAGCA
  GCAGCCTGTCTTGTGTGAGGTGTTCTGCCGGGATAGAAAGGTGCTTCGGGAGGGGGAGAGACTGACCCTG
  CCGCAGCTGGCTGACACCTACGAGACGCTGGCCATCGAGGGTGCCCAGGCCTTCTACAACGGCAGCCTCA
  CGGCCCAGATTGTGAAGGACATCCAGGCGGCCGGGGGCATTGTGACAGCTGAGGACCTGAACAACTACCG
  TGCTGAGCTGATCGAGCACCCGCTGAACATCAGCCTGGGAGACGTGGTGCTGTACATGCCCAGTGCGCCG
  CTCAGCGGGCCCGTGCTGGCCCTCATCCTCAACATCCTCAAAGGGTACAACTTCTCCCGGGAGAGCGTGG
  AGAGCCCCGAGCAGAAGGGCCTGACGTACCACCGCATCGTAGAGGCTTTCCGGTTTGCCTACGCCAAGAG
  GACCCTGCTTGGGGACCCCAAGTTTGTGGATGTGACTGAGGTGGTCCGCAACATGACCTCCGAGTTCTTC
  GCTGCCCAGCTCCGGGCCCAGATCTCTGACGACACCACTCACCCGATCTCCTACTACAAGCCCGAGTTCT
  ACACGCCGGATGACGGGGGCACTGCTCACCTGTCTGTCGTCGCAGAGGACGGCAGTGCTGTGTCCGCCAC
  CAGCACCATCAACCTCTACTTTGGCTCCAAGGTCCGCTCCCCGGTCAGCGGGATCCTGTTCAATAATGAA
  ATGGACGACTTCAGCTCTCCCAGCATCACCAACGAGTTTGGGGTACCCCCCTCACCTGCCAATTTCATCC
  AGCCAGGGAAGCAGCCGCTCTCGTCCATGTGCCCGACGATCATGGTGGGCCAGGACGGCCAGGTCCGGAT
  GGTGGTGGGAGCTGCTGGGGGCACACAGATCACCACGGCCACTGCACTGGCCATCATCTACAACCTCTGG
  TTCGGCTATGACGTGAAGCGGGCCGTGGAGGAGCCCCGGCTGCACAACCAGCTTCTGCCCAACGTCACGA
  CAGTGGAGAGAAACATTGACCAGGCAGTGACTGCAGCCCTGGAGACCCGGCACCATCACACCCAGATCGC
  GTCCACCTTCATCGCTGTGGTGCAAGCCATCGTCCGCACGGCTGGTGGCTGGGCAGCTGCCTCGGACTCC
  AGGAAAGGCGGGGAGCCTGCCGGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223640 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKKLVVLGLLAVVLVLVIVGLCLWLPSASKEPDNHVYTRAAVAADAKQCSKIGRDALRDGGSAVDAAIA
  ALLCVGLMNAHSMGIGGGLFLTIYNSTTRKAEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRG
  YELAHQRHGRLPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDRKVLREGERLTL
  PQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVTAEDLNNYRAELIEHPLNISLGDVVLYMPSAP
  LSGPVLALILNILKGYNFSRESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEFF
  AAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNE
  MDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLW
  FGYDVKRAVEEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRTAGGWAAASDS
  RKGGEPAGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013430
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013430.2, NP_038347.2
• RefSeq Size: 2381 bp
• RefSeq ORF: 1710 bp
• Locus ID: 2678
• UniProt ID: P19440
• Cytogenetics: 22q11.23
• Domains: G_glu_transpept
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• MW: 61.4 kDa
• Gene Summary: The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]