ADAM22 (NM_016351) Human Tagged ORF Clone

CAT#: RC223610

ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3

Product Images

Circular map for RC223610

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM22
• Synonyms: ADAM 22; DEE61; EIEE61; MDC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223610 representing NM_016351.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGGCGGCAGTGGCTGTGTCCGTGCCCTTCTTGCTGCTCTGTGTCCTGGGGACCTGCCCTCCGGCG
  CGCTGCGGCCAGGCAGGAGACGCCTCATTGATGGAGCTAGAGAAGAGGAAGGAAAACCGCTTCGTGGAG
  CGCCAGAGCATCGTGCCACTGCGCCTCATCTACCGCTCGGGCGGCGAAGACGAAAGTCGGCACGACGCG
  CTCGACACGCGGGTGCGGGGCGACCTCGGTGGCCCGCAGTTGACTCATGTTGACCAAGCAAGCTTCCAG
  GTTGATGCCTTTGGAACGTCATTCATTCTCGATGTCGTGCTAAATCATGATTTGCTGTCCTCTGAATAC
  ATAGAGAGACACATTGAACATGGAGGCAAGACTGTGGAAGTTAAAGGAGGAGAGCACTGTTACTACCAG
  GGCCATATCCGAGGAAACCCTGACTCATTTGTTGCATTGTCAACATGCCACGGACTTCATGGGATGTTC
  TATGACGGGAACCACACATATCTCATTGAGCCAGAAGAAAATGACACTACTCAAGAGGATTTCCATTTT
  CATTCAGTTTACAAATCCAGACTGTTTGAATTTTCCTTGGATGATCTTCCATCTGAATTTCAGCAAGTA
  AACATTACTCCATCAAAATTTATTTTGAAGCCAAGACCAAAAAGGAGTAAACGGCAGCTTCGTCGATAT
  CCTCGTAATGTAGAAGAAGAAACCAAATACATTGAACTGATGATTGTGAATGATCACCTTATGTTTAAA
  AAACATCGGCTTTCCGTTGTACATACCAATACCTATGCGAAATCTGTGGTGAACATGGCAGATTTAATA
  TATAAAGACCAACTTAAGACCAGGATAGTATTGGTTGCTATGGAAACCTGGGCGACTGACAACAAGTTT
  GCCATATCTGAAAATCCATTGATCACCCTACGTGAGTTTATGAAATACAGGAGGGATTTTATCAAAGAG
  AAAAGTGATGCAGTTCACCTTTTTTCGGGAAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATT
  GGTGGGATTTGCTCGTTGCTGAAAGGAGGAGGCGTGAATGAATTTGGGAAAACTGATTTAATGGCTGTT
  ACACTTGCCCAGTCATTAGCCCATAATATTGGTATTATCTCAGACAAAAGAAAGTTAGCAAGTGGTGAA
  TGTAAATGCGAGGACACGTGGTCCGGGTGCATAATGGGAGACACTGGCTATTATCTTCCTAAAAAGTTC
  ACCCAGTGTAATATTGAAGAGTATCATGACTTCCTGAATAGTGGAGGTGGTGCCTGCCTTTTCAACAAA
  CCTTCTAAGCTTCTTGATCCTCCTGAGTGTGGCAATGGCTTCATTGAAACTGGAGAGGAGTGTGATTGT
  GGAACCCCGGCCGAATGTGTCCTTGAAGGAGCAGAGTGTTGTAAGAAATGCACCTTGACTCAAGACTCT
  CAATGCAGTGACGGTCTTTGCTGTAAAAAGTGCAAGTTTCAGCCTATGGGCACTGTGTGCCGAGAAGCA
  GTAAATGATTGTGATATTCGTGAAACGTGCTCAGGAAATTCAAGCCAGTGTGCCCCTAATATTCATAAA
  ATGGATGGATATTCATGTGATGGTGTTCAGGGAATTTGCTTTGGAGGAAGATGCAAAACCAGAGATAGA
  CAATGCAAATACATTTGGGGGCAAAAGGTGACAGCATCAGACAAATATTGCTATGAGAAACTGAATATT
  GAAGGGACGGAGAAGGGTAACTGTGGGAAAGACAAAGACACATGGATACAGTGCAACAAACGGGATGTG
  CTTTGTGGTTACCTTTTGTGTACCAATATTGGCAATATCCCAAGGCTTGGAGAACTCGATGGTGAAATC
  ACATCTACTTTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGTGGGCATGTTAAGCTTGAAGAA
  GATGTAGATCTTGGCTATGTGGAAGATGGGACACCTTGTGGTCCCCAAATGATGTGCTTAGAACACAGG
  TGTCTTCCTGTGGCTTCTTTCAACTTTAGTACTTGCTTGAGCAGTAAAGAAGGCACTATTTGCTCAGGA
  AATGGAGTTTGCAGTAATGAGCTGAAGTGTGTGTGTAACAGACACTGGATAGGTTCTGATTGCAACACT
  TACTTCCCTCACAATGATGATGCAAAGACTGGTATCACTCTGTCTGGCAATGGTGTTGCTGGCACCAAT
  ATCATAATAGGCATAATTGCTGGCACCATTTTAGTGCTGGCCCTCATATTAGGAATAACTGCGTGGGGT
  TATAAAAACTATCGAGAACAGAGGTCAAATGGGCTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACA
  AAACATATTTCAGACATCTGTGAAAATGGGCGACCTCGAAGTAACTCTTGGCAAGGTAACCTGGGAGGC
  AACAAAAAGAAAATCAGAGGCAAAAGATTTAGACCTCGGTCTAATTCAACTGAGACTTTATCTCCTGCC
  AAGTCTCCTTCTTCATCAACTGGGTCTATTGCCTCCAGCAGAAAATACCCTTACCCAATGCCTCCACTT
  CCTGATGAGGACAAGAAAGTGAACCGACAAAGTGCCAGGCTATGGGAGACATCCATT
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC223610
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQAAVAVSVPFLLLCVLGTCPPARCGQAGDASLMELEKRKENRFVERQSIVPLRLIYRSGGEDESRHDA
  LDTRVRGDLGGPQLTHVDQASFQVDAFGTSFILDVVLNHDLLSSEYIERHIEHGGKTVEVKGGEHCYYQ
  GHIRGNPDSFVALSTCHGLHGMFYDGNHTYLIEPEENDTTQEDFHFHSVYKSRLFEFSLDDLPSEFQQV
  NITPSKFILKPRPKRSKRQLRRYPRNVEEETKYIELMIVNDHLMFKKHRLSVVHTNTYAKSVVNMADLI
  YKDQLKTRIVLVAMETWATDNKFAISENPLITLREFMKYRRDFIKEKSDAVHLFSGSQFESSRSGAAYI
  GGICSLLKGGGVNEFGKTDLMAVTLAQSLAHNIGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKF
  TQCNIEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECVLEGAECCKKCTLTQDS
  QCSDGLCCKKCKFQPMGTVCREAVNDCDIRETCSGNSSQCAPNIHKMDGYSCDGVQGICFGGRCKTRDR
  QCKYIWGQKVTASDKYCYEKLNIEGTEKGNCGKDKDTWIQCNKRDVLCGYLLCTNIGNIPRLGELDGEI
  TSTLVVQQGRTLNCSGGHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCLSSKEGTICSG
  NGVCSNELKCVCNRHWIGSDCNTYFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWG
  YKNYREQRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNLGGNKKKIRGKRFRPRSNSTETLSPA
  KSPSSSTGSIASSRKYPYPMPPLPDEDKKVNRQSARLWETSI
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016351
• ORF Size: 2610 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016351.6
• RefSeq Size: 9226 bp
• RefSeq ORF: 2613 bp
• Locus ID: 53616
• Cytogenetics: 7q21.12
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 96.6 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]