ADAM22 (NM_016351) Human Tagged ORF Clone
ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3
|Product Name||ADAM22 (NM_016351) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ADAM 22; EIEE61; MDC2|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||2613 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_016351.1, NM_016351.2, NM_016351.3, NM_016351.4, NM_016351.5, NP_057435.2|
|RefSeq Size||9226 bp|
|RefSeq ORF||2613 bp|
|Domains||Reprolysin, DISIN, Pep_M12B_propep, ACR|
|Protein Families||Druggable Genome, Protease, Transmembrane|
|Gene Summary||This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants. [provided by RefSeq, Dec 2010]|
|The use of this cDNA Clones has been cited in the following citations:|
ADAM22 and ADAM23 modulate the targeting of the Kv1 channel-associated protein LGI1 to the axon initial segment
,Hivert, B;Marien, L;Agbam, K;Faivre-Sarrailh, C;,
Inhibitory axons are targeted in hippocampal cell culture by anti-Caspr2 autoantibodies associated with limbic encephalitis
,Pinatel, D;Hivert, B;Boucraut, J;Saint-Martin, M;Rogemond, V;Zoupi, L;Karagogeos, D;Honnorat, J;Faivre-Sarrailh, C;,
Front Cell Neurosci Jul 2015
,PubMed ID 26217189
|cDNA Clone Resources|
|SC310071||ADAM22 (untagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||
|RG223610||ADAM22 (GFP-tagged) - Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||
|RC223610L1||Lenti-ORF clone of ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||
|RC223610L2||Lenti-ORF clone of ADAM22 (mGFP-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||
|RC223610L3||Lenti-ORF clone of ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||
|RC223610L4||Lenti-ORF clone of ADAM22 (mGFP-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3||