ATRIP (NM_130384) Human Tagged ORF Clone

CAT#: RC223562

ATRIP (Myc-DDK-tagged)-Human ATR interacting protein (ATRIP), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ATRIP (Cat# RC223562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP(Cat# TA504641). Positive lysates LY408977 (100ug) and LC408977 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY408977) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ATRIP protein (Cat# TP323562). The protein was produced from HEK293T cells transfected with ATRIP cDNA clone (Cat# RC223562) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223562 representing NM_130384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGACCTCCGCGCCAGGCAGCAAGAGGCGGAGCGAGCCCCCGGCGCCTCGCCCCGGCCCGCCGC
  CGGGCACCGGGCACCCCCCGAGCAAGCGGGCCCGGGGCTTCTCCGCAGCCGCTGCCCCGGACCCTGACGA
  CCCGTTCGGCGCGCATGGGGACTTCACTGCCGACGACCTGGAGGAGCTTGACACCCTCGCGTCACAGGCC
  CTGAGCCAATGTCCGGCCGCGGCTCGGGACGTGTCCAGTGATCATAAGGTCCACAGATTATTAGATGGCA
  TGTCAAAAAATCCTTCAGGGAAAAACAGAGAAACTGTTCCAATTAAAGATAATTTCGAATTAGAGGTACT
  TCAGGCACAATACAAAGAACTTAAAGAAAAGATGAAAGTAATGGAAGAAGAAGTTCTCATTAAGAATGGA
  GAAATTAAAATTTTGCGAGACTCACTACATCAGACGGAATCCGTTCTAGAGGAACAGAGAAGATCACATT
  TTCTTCTTGAGCAAGAGAAAACCCAAGCACTCAGTGACAAGGAAAAGGAATTCTCCAAAAAGCTCCAATC
  ATTGCAGTCTGAACTCCAGTTTAAAGATGCAGAGATGAATGAATTAAGGACAAAGCTCCAGACCAGTGAA
  CGAGCAAATAAACTGGCTGCTCCCTCTGTTTCCCATGTCAGTCCTAGGAAAAACCCTTCTGTGGTTATAA
  AGCCAGAAGCATGTTCTCCACAATTTGGAAAAACATCTTTTCCTACAAAGGAGTCTTTTAGTGCTAACAT
  GTCCCTTCCCCACCCCTGCCAGACGGAGTCAGGATACAAGCCTCTGGTGGGCAGAGAGGATAGTAAGCCC
  CACAGTCTGAGAGGTGACTCCATAAAACAAGAAGAGGCCCAGAAAAGCTTTGTTGACAGCTGGAGACAGA
  GATCAAACACTCAAGGTTCCATTTTGATAAACCTGCTCCTGAAGCAGCCTTTGATCCCAGGGTCATCCCT
  AAGCCTTTGCCACCTCCTGAGTAGTAGTTCTGAGTCTCCTGCTGGCACCCCCCTGCAGCCACCAGGGTTT
  GGCAGTACCTTGGCTGGAATGTCAGGCCTCAGGACCACAGGTTCTTATGATGGGTCATTTTCCCTCTCAG
  CCCTGAGAGAAGCACAGAACCTGGCATTCACTGGACTGAATCTGGTTGCCCGGAATGAGTGCTCACGTGA
  TGGAGACCCAGCAGAGGGAGGCAGAAGGGCCTTCCCACTCTGCCAGCTTCCTGGAGCCGTGCATTTCCTC
  CCCCTTGTACAGTTCTTCATCGGCTTACACTGCCAGGCCCTGCAGGACTTGGCAGCTGCTAAGAGAAGCG
  GAGCACCTGGGGACTCACCGACACATTCCTCCTGCGTGAGCTCTGGGGTAGAGACCAACCCTGAGGACTC
  AGTGTGCATCCTGGAAGGCTTCTCTGTGACTGCACTTAGCATTCTTCAGCACCTGGTGTGCCACAGCGGA
  GCAGTCGTCTCCCTATTACTGTCAGGAGTGGGGGCAGATTCTGCTGCTGGGGAAGGAAACAGGAGCCTGG
  TTCACAGGCTTAGTGATGGAGATATGACCTCAGCCCTAAGGGGGGTTGCTGATGACCAAGGACAGCACCC
  ACTGTTGAAGATGCTTCTTCACCTGTTGGCTTTCTCTTCTGCAGCAACAGGTCACCTTCAAGCCAGTGTC
  CTGACCCAGTGCCTTAAGGTTTTGGTGAAATTAGCCGAAAACACTTCCTGTGATTTCTTGCCCAGGTTCC
  AGTGTGTGTTCCAAGTGCTGCCAAAGTGCCTCAGCCCAGAGACACCCCTGCCTAGCGTGCTGCTGGCTGT
  TGAGCTCCTCTCCCTGCTGGCGGACCACGACCAGCTGGCACCTCAGCTCTGTTCCCACTCAGAAGGCTGC
  CTCCTGCTGCTGCTGTACATGTACATCACATCACGGCCTGACAGAGTGGCCTTGGAGACACAATGGCTCC
  AGCTGGAACAAGAGGTGGTGTGGCTCCTGGCTAAGCTTGGTGTGCAGAGCCCCTTGCCCCCAGTCACTGG
  CTCCAACTGCCAGTGTAATGTGGAGGTGGTCAGAGCGCTCACGGTGATGTTGCACAGACAGTGGCTGACA
  GTGCGGAGGGCAGGGGGACCCCCAAGGACCGACCAGCAGAGGCGGACAGTGCGCTGTCTGCGGGACACGG
  TGCTGCTGCTGCACGGCCTATCGCAGAAGGACAAGCTCTTCATGATGCACTGCGTGGAGGTCCTGCATCA
  GTTTGACCAGGTGATGCCGGGGGTCAGCATGCTCATCCGAGGGCTTCCTGATGTGACGGACTGTGAAGAG
  GCAGCCCTGGATGACCTCTGTGCCGCGGAAACCGATGTGGAAGACCCCGAGGTGGAGTGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223562 representing NM_130384
  Red=Cloning site Green=Tags(s)
  
  MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQA
  LSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNG
  EIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSE
  RANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKP
  HSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGF
  GSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFL
  PLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSG
  AVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASV
  LTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGC
  LLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLT
  VRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEE
  AALDDLCAAETDVEDPEVECG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130384
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130384.3
• RefSeq Size: 2509 bp
• RefSeq ORF: 2376 bp
• Locus ID: 84126
• UniProt ID: Q8WXE1
• Cytogenetics: 3p21.31
• MW: 85.7 kDa
• Gene Summary: This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]