JMJD7-PLA2G4B (NM_005090) Human Tagged ORF Clone

CAT#: RC223316

JMJD7 (Myc-DDK-tagged)-Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1

Product Images

Circular map for RC223316

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  Western blot validation of overexpression lysate (Cat# LY417529) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223316 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified JMJD7-PLA2G4B protein (Cat# TP323316). The protein was produced from HEK293T cells transfected with JMJD7-PLA2G4B cDNA clone (Cat# RC223316) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JMJD7-PLA2G4B
• Synonyms: cPLA2-beta; HsT16992
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223316 representing NM_005090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGGCTTTGGAAGCCGTGCGGAGCGAGTTACGAGAATTCCCGGCCGCTGCAAGGGAGCTCT
  GCGTGCCTCTTGCTGTGCCCTACCTGGACAAACCCCCAACTCCGCTCCACTTCTACCGGGACTGGGTCTG
  CCCCAACAGGCCGTGCATTATCCGCAACGCTCTGCAGCACTGGCCGGCCCTCCAGAAGTGGTCCCTCCCC
  TATTTCAGAGCCACAGTGGGCTCCACAGAGGTGAGTGTGGCCGTGACCCCAGATGGTTACGCGGATGCCG
  TGAGAGGGGATCGCTTCATGATGCCAGCTGAGCGCCGCCTGCCCCTGAGCTTCGTGCTGGATGTGCTGGA
  GGGCCGGGCCCAGCACCCTGGAGTCCTCTATGTGCAGAAGCAGTGCTCCAACCTGCCCAGCGAGCTGCCC
  CAGCTGCTGCCTGATCTGGAATCCCATGTGCCCTGGGCCTCCGAAGCCCTGGGAAAGATGCCCGATGCTG
  TGAACTTCTGGCTGGGGGAGGCGGCTGCAGTGACTTCTTTGCACAAGGACCACTATGAGAACCTCTACTG
  CGTGGTCTCAGGAGAGAAGCATTTCCTGTTCCATCCGCCCAGCGACCGGCCCTTCATCCCCTATGAGCTG
  TACACGCCGGCAACCTACCAGCTAACTGAAGAGGGCACCTTTAAGGTGGTGGATGAAGAGGCCATGGAGA
  AGGCAGAGGTGTCCAGGACCTGCCTGCTCACGGTTCGTGTCCTGCAGGCCCATCGCCTACCCTCTAAGGA
  CCTAGTGACCCCCTCTGACTGCTACGTGACTCTCTGGCTGCCCACGGCCTGCAGCCACAGGCTCCAGACA
  CGCACGGTCAAGAACAGCAGTAGCCCTGTCTGGAACCAGAGCTTTCACTTCAGGATCCACAGGCAGCTCA
  AGAATGTCATGGAACTGAAAGTCTTTGACCAGGACCTGGTGACCGGAGATGACCCTGTGTTGTCAGTACT
  GTTTGATGCGGGGACTCTGCGGGCTGGGGAGTTCCGGCGCGAGAGCTTCTCACTGAGCCCTCAGGGTGAG
  GGGCGCCTGGAAGTTGAATTTCGCCTGCAGAGTCTGGCTGACCGTGGCGAGTGGCTCGTCAGCAATGGCG
  TTCTGGTGGCCCGGGAGCTCTCCTGCTTGCACGTTCAACTGGAGGAGACAGGAGACCAGAAGTCCTCAGA
  GCACAGAGTTCAGCTTGTGGTTCCTGGGTCCTGTGAGGGTCCGCAGGAGGCCTCTGTGGGCACTGGCACC
  TTCTGCTTCCACTGCCCAGCCTGCTGGGAGCAGGAGCTGAGTATTCGCCTGCAGGATGCCCCCGAGGAGC
  AACTAAAGGCGCCACTGAGTGCCCTGCCCTCTGGTCAAGTGGTGAGGCTTGTCTTCCCCACGTCCCAGGA
  GCCCCTGATGAGAGTGGAGCTGAAAAAAGAAGCAGGACTGAGGGAGCTGGCCGTGCGACTGGGCTTCGGG
  CCCTGTGCAGAGGAGCAGGCCTTCCTGAGCAGGAGGAAGCAGGTGGTGGCCGCGGCCTTGAGGCAGGCCC
  TGCAGCTGGATGGAGACCTGCAGGAGGATGAGATCCCAGTGGTAGCTATTATGGCCACTGGTGGTGGGAT
  CCGGGCAATGACTTCCCTGTATGGGCAGCTGGCTGGCCTGAAGGAGCTGGGCCTCTTGGATTGCGTCTCC
  TACATCACCGGGGCCTCGGGCTCCACCTGGGCCTTGGCCAACCTTTATGAGGACCCAGAGTGGTCTCAGA
  AGGACCTGGCAGGGCCCACTGAGTTGCTGAAGACCCAGGTGACCAAGAACAAGCTGGGTGTGCTGGCCCC
  CAGCCAGCTGCAGCGGTACCGGCAGGAGCTGGCCGAGCGTGCCCGCTTGGGCTACCCAAGCTGCTTCACC
  AACCTGTGGGCCCTCATCAACGAGGCGCTGCTGCATGATGAGCCCCATGATCACAAGCTCTCAGATCAAC
  GGGAGGCCCTGAGTCATGGCCAGAACCCTCTGCCCATCTACTGTGCCCTCAACACCAAAGGGCAGAGCCT
  GACCACTTTTGAATTTGGGGAGTGGTGCGAGTTCTCTCCCTACGAGGTCGGCTTCCCCAAGTACGGGGCC
  TTCATCCCCTCTGAGCTCTTTGGCTCCGAGTTCTTTATGGGGCAGCTGATGAAGAGGCTTCCTGAGTCCC
  GCATCTGCTTCTTAGAAGGTATCTGGAGCAACCTGTATGCAGCCAACCTCCAGGACAGCTTATACTGGGC
  CTCAGAGCCCAGCCAGTTCTGGGACCGCTGGGTCAGGAACCAGGCCAACCTGGACAAGGAGCAGGTCCCC
  CTTCTGAAGATAGAAGAACCACCCTCAACAGCCGGCAGGATAGCTGAGTTTTTCACCGATCTTCTGACGT
  GGCGTCCACTGGCCCAGGCCACACATAATTTCCTGCGTGGCCTCCATTTCCACAAAGACTACTTTCAGCA
  TCCTCACTTCTCCACATGGAAAGCTACCACTCTGGATGGGCTCCCCAACCAGCTGACACCCTCGGAGCCC
  CACCTGTGCCTGCTGGATGTTGGCTACCTCATCAATACCAGCTGCCTGCCCCTCCTGCAGCCCACTCGGG
  ACGTGGACCTCATCCTGTCATTGGACTACAACCTCCACGGAGCCTTCCAGCAGTTGCAGCTCCTGGGCCG
  GTTCTGCCAGGAGCAGGGGATCCCGTTCCCACCCATCTCGCCCAGCCCCGAAGAGCAGCTCCAGCCTCGG
  GAGTGCCACACCTTCTCCGACCCCACCTGCCCCGGAGCCCCTGCGGTGCTGCACTTTCCTCTGGTCAGCG
  ACTCCTTCCGGGAGTACTCGGCCCCTGGGGTCCGGCGGACACCCGAGGAGGCGGCAGCTGGGGAGGTGAA
  CCTGTCTTCATCGGACTCTCCCTACCACTACACGAAGGTGACCTACAGCCAGGAGGACGTGGACAAGCTG
  CTGCACCTGACACATTACAATGTCTGCAACAACCAGGAGCAGCTGCTGGAGGCTCTGCGCCAGGCAGTGC
  AGCGGAGGCGGCAGCGCAGGCCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223316 representing NM_005090
  Red=Cloning site Green=Tags(s)
  
  MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLP
  YFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELP
  QLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYEL
  YTPATYQLTEEGTFKVVDEEAMEKAEVSRTCLLTVRVLQAHRLPSKDLVTPSDCYVTLWLPTACSHRLQT
  RTVKNSSSPVWNQSFHFRIHRQLKNVMELKVFDQDLVTGDDPVLSVLFDAGTLRAGEFRRESFSLSPQGE
  GRLEVEFRLQSLADRGEWLVSNGVLVARELSCLHVQLEETGDQKSSEHRVQLVVPGSCEGPQEASVGTGT
  FCFHCPACWEQELSIRLQDAPEEQLKAPLSALPSGQVVRLVFPTSQEPLMRVELKKEAGLRELAVRLGFG
  PCAEEQAFLSRRKQVVAAALRQALQLDGDLQEDEIPVVAIMATGGGIRAMTSLYGQLAGLKELGLLDCVS
  YITGASGSTWALANLYEDPEWSQKDLAGPTELLKTQVTKNKLGVLAPSQLQRYRQELAERARLGYPSCFT
  NLWALINEALLHDEPHDHKLSDQREALSHGQNPLPIYCALNTKGQSLTTFEFGEWCEFSPYEVGFPKYGA
  FIPSELFGSEFFMGQLMKRLPESRICFLEGIWSNLYAANLQDSLYWASEPSQFWDRWVRNQANLDKEQVP
  LLKIEEPPSTAGRIAEFFTDLLTWRPLAQATHNFLRGLHFHKDYFQHPHFSTWKATTLDGLPNQLTPSEP
  HLCLLDVGYLINTSCLPLLQPTRDVDLILSLDYNLHGAFQQLQLLGRFCQEQGIPFPPISPSPEEQLQPR
  ECHTFSDPTCPGAPAVLHFPLVSDSFREYSAPGVRRTPEEAAAGEVNLSSSDSPYHYTKVTYSQEDVDKL
  LHLTHYNVCNNQEQLLEALRQAVQRRRQRRPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005090
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005090.3
• RefSeq Size: 3352 bp
• RefSeq ORF: 3039 bp
• Locus ID: 8681
• UniProt ID: P0C869
• Cytogenetics: 15q15.1
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 113.9 kDa
• Gene Summary: This locus represents naturally-occurring readthrough transcription between the neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2, group IVB (cytosolic) (PLA2G4B) genes. Readthrough transcripts encode fusion proteins that share amino acid sequence with each individual gene product, including a partial JmjC domain and downstream C2 and phospholipase A2 domains. Alternatively spliced transcript variants have been observed. [provided by RefSeq, Oct 2013]