XPF (ERCC4) (NM_005236) Human Tagged ORF Clone

CAT#: RC223300

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ERCC4 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4)



  "NM_005236" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 851.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ERCC4
Synonyms ERCC11; FANCQ; RAD1; XFEPS; XPF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223300 representing NM_005236
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAGGGCAGCCGGCTCGACGGATTGCCATGGCGCCGCTGCTGGAGTACGAGCGACAGCTGGTGC
TGGAACTGCTCGACACTGACGGGCTAGTAGTGTGCGCCCGCGGGCTCGGCGCGGACCGGCTCCTCTACCA
CTTTCTCCAGCTGCACTGCCACCCAGCCTGCCTGGTGCTGGTGCTCAACACGCAGCCGGCCGAGGAGGAG
TATTTTATCAATCAGCTGAAGATAGAAGGAGTTGAACACCTCCCTCGCCGTGTAACAAATGAAATCACAA
GCAACAGTCGCTATGAAGTTTACACACAAGGTGGTGTTATATTTGCGACAAGTAGGATACTTGTGGTTGA
CTTCTTGACTGATAGAATACCTTCAGATTTAATTACTGGCATCTTGGTGTATAGAGCCCACAGAATAATC
GAGTCTTGTCAAGAAGCATTCATCTTGCGCCTCTTTCGCCAGAAAAACAAACGTGGTTTTATTAAAGCTT
TCACAGACAATGCTGTTGCCTTTGATACTGGTTTTTGTCATGTGGAAAGAGTGATGAGAAATCTTTTTGT
GAGGAAACTGTATCTGTGGCCAAGGTTCCATGTAGCAGTAAACTCATTTTTAGAACAGCACAAACCTGAA
GTTGTAGAAATCCATGTTTCTATGACACCTACCATGCTTGCTATACAGACTGCTATACTGGACATTTTAA
ATGCATGTCTAAAGGAACTAAAATGCCATAACCCATCGCTTGAAGTGGAAGATTTATCTTTAGAAAATGC
TATTGGAAAACCTTTTGACAAGACAATCCGCCATTATCTGGATCCTTTGTGGCACCAGCTTGGAGCCAAG
ACTAAATCCTTAGTTCAGGATTTGAAGATATTACGAACTTTGCTGCAGTATCTCTCTCAGTATGATTGTG
TCACATTTCTTAATCTTCTGGAATCTCTGAGAGCAACGGAAAAAGCTTTTGGTCAGAATTCAGGTTGGCT
GTTTCTTGACTCCAGCACCTCGATGTTTATAAATGCTCGAGCAAGGGTTTATCATCTTCCAGATGCCAAA
ATGAGTAAAAAAGAAAAAATATCTGAAAAAATGGAAATTAAAGAAGGGGAAGAAACAAAAAAGGAACTGG
TCCTAGAAAGCAACCCAAAGTGGGAGGCACTGACTGAAGTATTAAAAGAAATTGAGGCAGAAAATAAGGA
GAGTGAAGCTCTTGGTGGTCCAGGTCAAGTACTGATTTGTGCAAGTGATGACCGAACATGTTCCCAGCTG
AGAGACTATATCACTCTTGGAGCGGAGGCCTTCTTATTGAGGCTCTACAGGAAAACCTTTGAGAAGGATA
GCAAAGCTGAAGAAGTCTGGATGAAATTTAGGAAGGAAGACAGTTCAAAGAGAATTAGGAAATCTCACAA
AAGACCTAAAGACCCCCAAAACAAAGAACGGGCTTCTACCAAAGAAAGAACCCTCAAAAAGAAAAAACGG
AAGTTGACCTTAACTCAAATGGTAGGAAAACCTGAAGAACTGGAAGAGGAAGGAGATGTCGAGGAAGGAT
ATCGTCGAGAAATAAGCAGTAGCCCAGAAAGCTGCCCGGAAGAAATTAAGCATGAAGAATTTGATGTAAA
TTTGTCATCGGATGCTGCTTTCGGAATCCTGAAAGAACCCCTCACTATCATCCATCCGCTTCTGGGTTGC
AGCGACCCCTATGCTCTGACAAGGGTACTACATGAAGTGGAGCCAAGATACGTGGTTCTTTATGACGCAG
AGCTAACCTTTGTTCGGCAGCTTGAAATTTACAGGGCGAGTAGGCCTGGGAAACCTCTGAGGGTTTACTT
TCTTATATACGGAGGTTCAACTGAGGAACAACGCTATCTCACTGCTTTGCGGAAAGAAAAGGAAGCTTTT
GAAAAACTCATAAGGGAAAAAGCAAGCATGGTTGTCCCTGAAGAAAGAGAAGGCAGAGATGAAACAAACT
TAGACCTAGTAAGAGGCACAGCATCTGCAGATGTTTCCACTGACACTCGGAAAGCCGGTGGCCAGGAACA
GAATGGTACACAGCAAAGCATAGTTGTGGATATGCGTGAATTTCGAAGTGAGCTTCCATCTCTGATCCAT
CGTCGGGGCATTGACATTGAACCCGTGACTTTAGAGGTTGGAGATTACATCCTCACTCCAGAAATGTGCG
TGGAGCGCAAGAGTATCAGTGATTTAATCGGCTCTTTAAATAACGGCCGCCTCTACAGCCAGTGCATCTC
CATGTCCCGCTACTACAAGCGTCCCGTGCTTCTGATTGAGTTTGACCCTAGCAAGCCTTTCTCTCTCACT
TCCCGAGGTGCCTTGTTTCAGGAGATCTCCAGCAATGACATTAGTTCCAAACTCACTCTTCTTACACTTC
ACTTCCCCAGACTACGGATTCTCTGGTGCCCCTCTCCTCATGCAACGGCGGAGTTGTTTGAGGAGCTGAA
ACAAAGCAAGCCACAGCCTGATGCGGCGACAGCACTGGCCATTACAGCAGATTCCGAAACCCTTCCCGAG
TCAGAGAAGTATAATCCTGGTCCCCAAGACTTCTTGTTAAAAATGCCAGGGGTGAATGCCAAAAACTGCC
GCTCCTTGATGCACCACGTTAAGAACATCGCAGAATTAGCAGCCCTGTCACAAGACGAGCTCACGAGTAT
TCTGGGGAATGCTGCAAATGCCAAACAGCTTTATGATTTCATTCACACCTCTTTTGCAGAAGTCGTATCA
AAAGGAAAAGGGAAAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223300 representing NM_005236
Red=Cloning site Green=Tags(s)

MESGQPARRIAMAPLLEYERQLVLELLDTDGLVVCARGLGADRLLYHFLQLHCHPACLVLVLNTQPAEEE
YFINQLKIEGVEHLPRRVTNEITSNSRYEVYTQGGVIFATSRILVVDFLTDRIPSDLITGILVYRAHRII
ESCQEAFILRLFRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPE
VVEIHVSMTPTMLAIQTAILDILNACLKELKCHNPSLEVEDLSLENAIGKPFDKTIRHYLDPLWHQLGAK
TKSLVQDLKILRTLLQYLSQYDCVTFLNLLESLRATEKAFGQNSGWLFLDSSTSMFINARARVYHLPDAK
MSKKEKISEKMEIKEGEETKKELVLESNPKWEALTEVLKEIEAENKESEALGGPGQVLICASDDRTCSQL
RDYITLGAEAFLLRLYRKTFEKDSKAEEVWMKFRKEDSSKRIRKSHKRPKDPQNKERASTKERTLKKKKR
KLTLTQMVGKPEELEEEGDVEEGYRREISSSPESCPEEIKHEEFDVNLSSDAAFGILKEPLTIIHPLLGC
SDPYALTRVLHEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAF
EKLIREKASMVVPEEREGRDETNLDLVRGTASADVSTDTRKAGGQEQNGTQQSIVVDMREFRSELPSLIH
RRGIDIEPVTLEVGDYILTPEMCVERKSISDLIGSLNNGRLYSQCISMSRYYKRPVLLIEFDPSKPFSLT
SRGALFQEISSNDISSKLTLLTLHFPRLRILWCPSPHATAELFEELKQSKPQPDAATALAITADSETLPE
SEKYNPGPQDFLLKMPGVNAKNCRSLMHHVKNIAELAALSQDELTSILGNAANAKQLYDFIHTSFAEVVS
KGKGKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005236
ORF Size 2748 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005236.3
RefSeq Size 2751 bp
RefSeq ORF 2751 bp
Locus ID 2072
UniProt ID Q92889
Cytogenetics 16p13.12
Protein Families Druggable Genome
Protein Pathways Nucleotide excision repair
MW 104.3 kDa
Gene Summary The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).[provided by RefSeq, Mar 2009]

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