ABCE1 (NM_002940) Human Tagged ORF Clone

CAT#: RC223295

ABCE1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family E (OABP), member 1 (ABCE1), transcript variant 1

Product Images

Circular map for RC223295

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  Western blot validation of overexpression lysate (Cat# LY401029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABCE1 protein (Cat# TP323295). The protein was produced from HEK293T cells transfected with ABCE1 cDNA clone (Cat# RC223295) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCE1
• Synonyms: ABC38; OABP; RLI; RLI1; RNASEL1; RNASELI; RNS4I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223295 representing NM_002940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACAAGTTAACGAGAATTGCTATTGTCAACCATGACAAATGTAAACCTAAGAAATGTCGACAGG
  AATGCAAAAAGAGTTGTCCTGTAGTTCGAATGGGAAAATTATGCATAGAGGTTACACCCCAGAGCAAAAT
  AGCATGGATTTCCGAAACTCTTTGTATTGGTTGTGGTATCTGTATTAAGAAATGCCCCTTTGGCGCCTTA
  TCAATTGTCAATCTACCAAGCAACTTGGAAAAAGAAACCACACATCGATATTGTGCCAATGCCTTCAAAC
  TTCACAGGTTGCCTATCCCTCGTCCAGGTGAAGTTTTGGGATTAGTTGGAACTAATGGTATTGGAAAGTC
  AACTGCTTTAAAAATTTTAGCAGGAAAACAAAAGCCAAACCTTGGAAAGTACGATGATCCTCCTGACTGG
  CAGGAGATTTTGACTTATTTCCGTGGATCTGAATTACAAAATTACTTTACAAAGATTCTAGAAGATGACC
  TAAAAGCCATCATCAAACCTCAATATGTAGACCAGATTCCTAAGGCTGCAAAGGGGACAGTGGGATCTAT
  TTTGGACCGAAAAGATGAAACAAAGACACAGGCAATTGTATGTCAGCAGCTTGATTTAACCCACCTAAAA
  GAACGAAATGTTGAAGATCTTTCAGGAGGAGAGTTGCAGAGATTTGCTTGTGCTGTCGTTTGCATACAGA
  AAGCTGATATTTTCATGTTTGATGAGCCTTCTAGTTACCTAGATGTCAAGCAGCGTTTAAAGGCTGCTAT
  TACTATACGATCTCTAATAAATCCAGATAGATATATCATTGTGGTGGAACATGATCTAAGTGTATTAGAC
  TATCTCTCCGACTTCATCTGCTGTTTATATGGTGTACCAAGCGCCTATGGAGTTGTCACTATGCCTTTTA
  GTGTAAGAGAAGGCATAAACATTTTTTTGGATGGCTATGTTCCAACAGAAAACTTGAGATTCAGAGATGC
  ATCACTTGTTTTTAAAGTGGCTGAGACAGCAAATGAAGAAGAAGTTAAAAAGATGTGTATGTATAAATAT
  CCAGGAATGAAGAAAAAAATGGGAGAATTTGAGCTAGCAATTGTAGCTGGAGAGTTTACAGATTCTGAAA
  TTATGGTGATGCTGGGGGAAAATGGAACGGGTAAAACGACATTTATCAGAATGCTTGCTGGAAGACTTAA
  ACCTGATGAAGGAGGAGAAGTACCAGTTCTAAATGTCAGTTATAAGCCACAGAAAATTAGTCCCAAATCA
  ACTGGAAGTGTTCGCCAGTTACTACATGAAAAGATAAGAGATGCTTATACTCACCCACAATTTGTGACCG
  ATGTAATGAAGCCTCTGCAAATTGAAAACATCATTGATCAAGAGGTGCAGACATTATCTGGTGGTGAACT
  ACAGCGAGTAGCTTTAGCCCTTTGCTTGGGCAAACCTGCTGATGTCTATTTAATTGATGAACCATCTGCA
  TATTTGGATTCTGAGCAAAGACTGATGGCAGCTCGAGTTGTCAAACGTTTCATACTCCATGCAAAAAAGA
  CAGCCTTTGTTGTGGAACATGACTTCATCATGGCCACCTATCTAGCGGATCGCGTCATCGTTTTTGATGG
  TGTTCCATCTAAGAACACAGTTGCAAACAGTCCTCAAACCCTTTTGGCTGGCATGAATAAATTTTTGTCT
  CAGCTTGAAATTACATTCAGAAGAGATCCAAACAACTATAGGCCACGAATAAACAAACTTAATTCAATTA
  AGGATGTAGAACAAAAGAAGAGTGGAAACTACTTTTTCTTGGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223295 representing NM_002940
  Red=Cloning site Green=Tags(s)
  
  MADKLTRIAIVNHDKCKPKKCRQECKKSCPVVRMGKLCIEVTPQSKIAWISETLCIGCGICIKKCPFGAL
  SIVNLPSNLEKETTHRYCANAFKLHRLPIPRPGEVLGLVGTNGIGKSTALKILAGKQKPNLGKYDDPPDW
  QEILTYFRGSELQNYFTKILEDDLKAIIKPQYVDQIPKAAKGTVGSILDRKDETKTQAIVCQQLDLTHLK
  ERNVEDLSGGELQRFACAVVCIQKADIFMFDEPSSYLDVKQRLKAAITIRSLINPDRYIIVVEHDLSVLD
  YLSDFICCLYGVPSAYGVVTMPFSVREGINIFLDGYVPTENLRFRDASLVFKVAETANEEEVKKMCMYKY
  PGMKKKMGEFELAIVAGEFTDSEIMVMLGENGTGKTTFIRMLAGRLKPDEGGEVPVLNVSYKPQKISPKS
  TGSVRQLLHEKIRDAYTHPQFVTDVMKPLQIENIIDQEVQTLSGGELQRVALALCLGKPADVYLIDEPSA
  YLDSEQRLMAARVVKRFILHAKKTAFVVEHDFIMATYLADRVIVFDGVPSKNTVANSPQTLLAGMNKFLS
  QLEITFRRDPNNYRPRINKLNSIKDVEQKKSGNYFFLDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002940
• ORF Size: 1797 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002940.3
• RefSeq Size: 3568 bp
• RefSeq ORF: 1800 bp
• Locus ID: 6059
• UniProt ID: P61221
• Cytogenetics: 4q31.21
• Domains: fer4, ABC_tran, AAA, RLI
• Protein Families: Druggable Genome
• MW: 67.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]