ALDH1A2 (NM_003888) Human Tagged ORF Clone

SKU RC223250

ALDH1A2 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ALDH1A2
• Synonyms: RALDH(II); RALDH2; RALDH2-T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223250 representing NM_003888; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACTTCCAGCAAGATAGAGATGCCCGGCGAGGTGAAGGCCGACCCCGCCGCCCTCATGGCGTCGCTGC; ACCTCCTGCCGTCGCCCACGCCCAATCTCGAAATTAAGTACACCAAGATCTTTATAAACAACGAGTGGCA; GAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTATAATCCAGCCACAGGAGAACAGGTGTGTGAAGTTCAA; GAAGCAGACAAGGCAGATATAGACAAAGCAGTGCAGGCAGCCCGCCTGGCTTTCTCTCTTGGTTCAGTGT; GGAGAAGGATGGATGCTTCAGAAAGGGGACGTCTGTTGGATAAGCTTGCAGACTTGGTGGAACGGGACAG; GGCAGTTCTTGCAACCATGGAATCCCTAAATGGTGGCAAACCATTCCTGCAAGCTTTTTATGTGGATTTG; CAGGGCGTCATCAAAACCTTTCGATATTACGCAGGCTGGGCTGATAAAATTCATGGGATGACCATTCCTG; TAGATGGAGACTATTTTACCTTTACAAGACATGAACCCATTGGAGTGTGTGGACAGATCATCCCATGGAA; CTTCCCCCTGCTGATGTTTGCCTGGAAAATAGCTCCAGCTTTGTGCTGTGGCAATACAGTAGTTATTAAG; CCAGCAGAGCAAACACCACTCAGTGCACTCTACATGGGAGCCCTCATCAAGGAGGCTGGCTTTCCTCCCG; GGGTCATCAATATTTTGCCAGGATATGGGCCAACGGCTGGGGCAGCAATAGCTTCTCACATTGGCATAGA; CAAGATTGCATTCACAGGGTCTACTGAGGTTGGAAAGCTTATCCAAGAAGCAGCTGGAAGAAGTAATTTG; AAGAGAGTAACTCTGGAACTTGGAGGCAAAAGTCCTAATATTATTTTTGCTGATGCTGACTTGGACTATG; CTGTGGAGCAGGCCCACCAGGGTGTGTTCTTCAATCAAGGTCAGTGCTGCACTGCAGGCTCTCGCATCTT; CGTGGAGGAGTCCATCTATGAGGAGTTTGTGAGAAGAAGCGTGGAGCGGGCCAAGAGGCGCGTAGTGGGG; AGTCCCTTTGACCCCACCACTGAGCAGGGTCCCCAGATTGATAAGAAACAGTACAACAAGATCTTGGAAC; TCATCCAGAGTGGTGTGGCTGAGGGCGCCAAGCTGGAATGTGGAGGCAAAGGACTGGGCCGAAAGGGGTT; TTTCATTGAGCCCACAGTGTTTTCCAACGTCACTGATGATATGCGGATTGCCAAGGAGGAGATCTTTGGC; CCTGTTCAGGAAATTTTGAGATTTAAGACGATGGATGAAGTTATCGAAAGAGCCAATAACTCAGACTTTG; GACTCGTAGCAGCTGTCTTTACTAATGACATCAACAAGGCCCTCACAGTGTCTTCTGCAATGCAAGCTGG; GACTGTTTGGATCAATTGTTACAATGCCTTAAATGCCCAGAGCCCCTTTGGGGGATTCAAGATGTCTGGA; AATGGGAGAGAAATGGGAGAATTTGGCTTGCGGGAGTACTCAGAAGTTAAGACGGTGACAGTAAAGATCC; CCCAGAAGAACTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223250 representing NM_003888; Red=Cloning site Green=Tags(s); ; MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQ; EADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDL; QGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIK; PAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNL; KRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVG; SPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG; PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSG; NGREMGEFGLREYSEVKTVTVKIPQKNS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003888
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003888.4
• RefSeq Size: 3398 bp
• RefSeq ORF: 1557 bp
• Locus ID: 8854
• UniProt ID: O94788
• Cytogenetics: 15q21.3
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism
• MW: 56.5 kDa
• Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011]