STARD4 (NM_139164) Human Tagged ORF Clone
Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 4 (STARD4), Myc-DDK-tagged
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||None|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||615 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2264 bp|
|RefSeq ORF||618 bp|
|Gene Summary||Cholesterol homeostasis is regulated, at least in part, by sterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM 184756) and by liver X receptors (e.g., LXRA; MIM 602423). Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers, such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain, including STARD4 (Soccio et al., 2002 [PubMed 12011452]).[supplied by OMIM, Mar 2008]|
|cDNA Clone Resources|
|RC223123||STARD4 (Myc-DDK-tagged)-Human StAR-related lipid transfer (START) domain containing 4 (STARD4)||
|RC223123L2||Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 4 (STARD4), mGFP tagged||
|RC223123L3||Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 4 (STARD4), Myc-DDK-tagged||
|RC223123L4||Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 4 (STARD4), mGFP tagged||
|RG223123||STARD4 (GFP-tagged) - Human StAR-related lipid transfer (START) domain containing 4 (STARD4)||
|SC120593||STARD4 (untagged)-Human StAR-related lipid transfer (START) domain containing 4 (STARD4)||