AJAP1 (NM_001042478) Human Tagged ORF Clone

CAT#: RC223102

AJAP1 (Myc-DDK-tagged)-Human adherens junctions associated protein 1 (AJAP1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AJAP1
• Synonyms: MOT8; SHREW-1; SHREW1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223102 representing NM_001042478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGATTCAACAGCTTTTAGGACTCAGCTCCATGTCCATCCGCTGGCCGGGCCGCCCCCTCGGAAGCC
  ATGCCTGGATACTGATAGCCATGTTTCAGCTCGCCGTGGACCTGCCCGCCTGTGAGGCCCTGGGCCCGGG
  GCCGGAGTTCTGGCTCCTGCCGCGGTCGCCGCCCCGGCCGCCCCGGCTGTGGAGTTTTAGGAGTGGACAG
  CCAGCGCGGGTCCCGGCCCCGGTGTGGAGCCCCCGGCCGCCCCGAGTGGAGCGGATCCACGGGCAGATGC
  AGATGCCTCGAGCCAGACGGGCCCACAGGCCCCGGGACCAGGCGGCCGCCCTCGTGCCCAAGGCAGGACT
  GGCCAAGCCCCCAGCTGCTGCCAAATCCAGCCCTTCCCTCGCCTCTTCGTCCTCGTCCTCGTCCTCCGCG
  GTGGCCGGTGGGGCCCCGGAGCAGCAGGCCCTCCTGAGGAGGGGCAAGAGGCACCTGCAGGGGGACGGTC
  TCAGCAGCTTCGACTCCAGAGGCAGCCGGCCCACCACAGAGACTGAGTTCATCGCCTGGGGGCCCACGGG
  GGACGAGGAGGCCCTGGAGTCCAACACATTTCCGGGCGTTTACGGCCCCACCACGGTCTCCATCCTACAA
  ACACGGAAGACAACTGTGGCCGCCACCACCACCACCACCACCACGGCCACCCCCATGACGCTGCAGACTA
  AGGGGTTCACCGAGTCCTTGGATCCCCGGAGAAGGATCCCAGGTGGGGTTAGCACAACGGAGCCTTCCAC
  CAGTCCCAGCAACAACGGGGAAGTCACCCAGCCCCCAAGGATTCTGGGGGAGGCCTCAGGTCTGGCTGTC
  CATCAGATCATCACCATCACCGTCTCCCTCATCATGGTCATAGCTGCTCTCATCACAACTCTTGTCTTAA
  AAAATTGCTGTGCCCAAAGCGGGAACACTCGTCGGAACAGCCACCAGCGGAAGACCAACCAGCAGGAGGA
  GAGCTGCCAGAACCTCACGGACTTCCCCTCGGCCCGGGTGCCCAGCAGCCTGGACATATTCACGGCCTAT
  AACGAGACCCTGCAGTGTTCTCACGAGTGCGTCAGGGCATCTGTGCCCGTGTACACCGATGAGACGCTGC
  ACTCGACGACGGGGGAGTACAAATCCACATTTAATGGAAACCGACCCTCCTCTTCTGATCGGCATCTTAT
  TCCTGTGGCCTTCGTGTCTGAGAAATGGTTTGAAATCTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223102 representing NM_001042478
  Red=Cloning site Green=Tags(s)
  
  MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQ
  PARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSA
  VAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQ
  TRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAV
  HQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAY
  NETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042478
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042478.2
• RefSeq Size: 2070 bp
• RefSeq ORF: 1236 bp
• Locus ID: 55966
• UniProt ID: Q9UKB5
• Cytogenetics: 1p36.32
• Protein Families: Druggable Genome, Transmembrane
• MW: 44.4 kDa
• Gene Summary: Plays a role in cell adhesion and cell migration.[UniProtKB/Swiss-Prot Function]