TAGAP (NM_152133) Human Tagged ORF Clone

CAT#: RC223030

TAGAP (Myc-DDK-tagged)-Human T-cell activation RhoGTPase activating protein (TAGAP), transcript variant 1

Product Images

Circular map for RC223030

• 

  Western blot validation of overexpression lysate (Cat# LY407719) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223030 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TAGAP protein (Cat# TP323030). The protein was produced from HEK293T cells transfected with TAGAP cDNA clone (Cat# RC223030) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAGAP
• Synonyms: ARHGAP47; FKSG15; IDDM21; TAGAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223030 representing NM_152133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCTCTGGAGATGCAGGACGAGGAGGACAGAATCGAGGCCCTGAAACAGGTTGCAGATAAGCTCC
  CCCGGCCCAACCTCCTGCTACTCAAGCACTTGGTCTATGTGCTGCACCTCATCAGCAAGAACTCTGAGGT
  GAACAGGATGGACTCCAGCAATCTGGCCATCTGCATTGGACCCAACATGCTCACCCTGGAGAATGACCAG
  AGCCTGTCATTTGAAGCCCAGAAGGACCTGAACAACAAGGTGAAGACACTGGTGGAATTCCTCATTGATA
  ACTGCTTTGAAATATTTGGGGAGAACATTCCAGTGCATTCCAGTATCACTTCTGATGACTCCCTGGAGCA
  CACTGACAGTTCAGATGTGTCGACCCTGCAGAATGACTCAGCCTACGACAGCAACGACCCTGATGTGGAA
  TCCAACAGCAGCAGTGGCATCAGCTCTCCCAGCAGGCAGCCCCAGGTGCCCATGGCCACAGCTGCTGGCT
  TGGATAGCGCGGGCCCACAGGATGCCCGAGAGGTCAGCCCAGAGCCCATTGTGAGCACCGTGGCCAGGCT
  GAAAAGCTCCCTCGCACAGCCCGATAGGAGATACTCAGAGCCCAGCATGCCATCCTCCCAGGAGTGCCTC
  GAGAGCCGGGTGACAAACCAAACACTAACAAAGAGTGAAGGGGACTTCCCCGTGCCCCGGGTAGGCTCTC
  GTTTGGAAAGTGAGGAGGCTGAAGACCCATTTCCAGAGGAGGTCTTCCCTGCAGTGCAAGGCAAAACCAA
  GAGGCCGGTGGACCTGAAGATCAAGAACTTGGCCCCGGGTTCGGTGCTCCCGCGGGCACTGGTTCTCAAA
  GCCTTCTCCAGCAGCTCGCTGGACGCGTCCTCTGACAGCTCGCCCGTGGCTTCTCCTTCCAGTCCCAAAA
  GAAATTTCTTCAGCAGACATCAGTCTTTCACCACAAAGACAGAGAAAGGCAAGCCCAGCCGAGAAATTAA
  AAAGCACTCCATGTCTTTCACCTTTGCCCCTCACAAAAAAGTGCTGACCAAAAACCTCAGCGCGGGCTCT
  GGGAAATCGCAAGACTTTACCAGGGACCACGTCCCGAGGGGTGTCAGAAAGGAAAGCCAGCTTGCCGGCC
  GAATCGTGCAGGAAAATGGGTGTGAAACCCACAACCAAACAGCCCGCGGCTTCTGCCTGAGACCCCACGC
  CCTCTCGGTGGATGATGTGTTCCAGGGAGCTGACTGGGAGAGGCCTGGAAGCCCACCCTCTTATGAAGAG
  GCCATGCAGGGCCCGGCAGCCAGACTAGTGGCCTCCGAGAGCCAGACCGTGGGGAGCATGACGGTGGGGA
  GCATGAGGGCGAGGATGCTGGAGGCGCACTGCCTCCTACCCCCTCTTCCACCTGCTCACCACGTAGAGGA
  CTCAAGACACAGGGGCAGCAAAGAGCCACTCCCTGGCCACGGACTCTCTCCCCTGCCTGAGCGATGGAAA
  CAGAGCAGAACTGTCCATGCTTCTGGGGACTCTCTGGGGCACGTGTCTGGCCCAGGGAGACCTGAGCTCC
  TCCCGCTGAGGACCGTCTCCGAGTCCGTGCAGAGGAATAAGCGGGACTGTCTCGTGCGACGATGTAGCCA
  GCCGGTCTTTGAGGCTGACCAATTCCAATATGCCAAAGAATCGTATATT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223030 representing NM_152133
  Red=Cloning site Green=Tags(s)
  
  MGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQ
  SLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVE
  SNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECL
  ESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLK
  AFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGS
  GKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEE
  AMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWK
  QSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152133
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152133.2
• RefSeq Size: 3196 bp
• RefSeq ORF: 1662 bp
• Locus ID: 117289
• UniProt ID: Q8N103
• Cytogenetics: 6q25.3
• MW: 60.5 kDa
• Gene Summary: This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]