SPTLC1 (NM_006415) Human Tagged ORF Clone

CAT#: RC222985

SPTLC1 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1

Product Images

Circular map for RC222985

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  Western blot validation of overexpression lysate (Cat# LY416663) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222985 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SPTLC1 protein (Cat# TP322985). The protein was produced from HEK293T cells transfected with SPTLC1 cDNA clone (Cat# RC222985) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPTLC1
• Synonyms: HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222985 representing NM_006415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCTCCTGCTTACC
  ATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTCTCTAAGACTTACAAATTACA
  AGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTGATTGAAGAGTGGCAACCAGAACCTCTTGTT
  CCTCCTGTCCCAAAAGACCATCCTGCTCTCAACTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTG
  TGGTGAATGGAAAAGAATGTATAAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGT
  TAAGGCAGCAGCTTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGC
  ACATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAAGCCATTATAT
  ACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAAAGAGGGGACATTGTTTTTGT
  AGATAGAGCTGCCTGCTTTGCTATTCAGAAAGGATTACAGGCATCCCGTAGTGACATTAAGTTATTTAAG
  CATAATGACATGGCTGACCTCGAGCGACTACTAAAAGAACAAGAGATCGAAGATCAAAAGAATCCTCGCA
  AGGCTCGTGTAACTCGGCGTTTCATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCT
  TCCAGAATTGGTTAAGTTAAAATACAAATACAAAGCAAGAATCTTCCTGGAGGAAAGCCTTTCATTTGGA
  GTCCTAGGAGAGCATGGCCGAGGAGTCACTGAACACTATGGAATCAATATTGATGATATTGATCTTATCA
  GTGCCAACATGGAGAATGCACTTGCTTCTATTGGAGGTTTCTGCTGTGGCAGGTCTTTTGTAATTGACCA
  TCAGCGACTTTCCGGCCAGGGATACTGCTTTTCAGCTTCGTTACCTCCCCTGTTAGCTGCTGCAGCAATT
  GAGGCCCTCAACATCATGGAAGAGAATCCAGGTATTTTTGCAGTGTTGAAGGAAAAGTGCGGACAAATTC
  ATAAAGCTTTACAAGGCATTTCTGGATTAAAAGTGGTGGGGGAGTCCCTTTCTCCAGCCTTTCACCTACA
  ACTGGAAGAGAGCACTGGGTCTCGCGAGCAAGATGTCAGACTGCTTCAGGAAATTGTAGATCAATGCATG
  AACAGAAGTATTGCATTAACTCAGGCGCGCTACTTGGAGAAAGAAGAGAAGTGTCTCCCTCCTCCCAGCA
  TTCGGGTTGTGGTCACGGTGGAACAAACAGAGGAAGAACTGGAGAGAGCTGCGTCCACCATCAAGGAGGT
  AGCCCAGGCCGTCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222985 representing NM_006415
  Red=Cloning site Green=Tags(s)
  
  MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLV
  PPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYG
  TFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFK
  HNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFG
  VLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAI
  EALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCM
  NRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006415
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006415.4
• RefSeq Size: 2780 bp
• RefSeq ORF: 1422 bp
• Locus ID: 10558
• UniProt ID: O15269
• Cytogenetics: 9q22.31
• Domains: aminotran_1_2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 52.6 kDa
• Gene Summary: This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013]