TRAF4AF1 (KNSTRN) (NM_033286) Human Tagged ORF Clone

SKU RC222981

KNSTRN (Myc-DDK-tagged)-Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TRAF4AF1
• Synonyms: C15orf23; HSD11; SKAP; TRAF4AF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222981 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCTCCCGAAGCCCCGCCCCTGGACAGAGTTTTCCGTACAACATGGCTGTCTACAGAGTGCGATT; CCCACCCACTTCCGCCTAGCTACCGGAAGTTTCTATTTGAAACCCAGGAGGCCGACTTAGCCGGTGGCAC; GACAGTTGCTGCAGGGAATCTTTTAAACGAGAGCGAGAAGGACTGCGGGCAGGACCGGCGGGCTCCTGGG; GTTCAGCCGTGCCTCCTCGTTACGATGACCAGTGTGGTTAAGACAGTGTATAGCCTGCAGCCCTCCTCTG; CGCTGAGCGGCGGCCAGCCGGCAGACACACAAACTCGGGCCACTTCTAAGAGTCTCTTACCTGTTAGGTC; CAAAGAAGTCGATGTTTCCAAACAGCTTCATTCAGGAGGTCCAGAGAATGATGTTACAAAAATCACCAAA; CTGAGACGAGAGAATGGGCAAATGAAAGCTACTGACACTGCCACCAGAAGGAATGTCAGAAAAGGCTACA; AACCACTGAGTAAGCAAAAATCAGAGGAAGAGCTCAAGGACAAGAACCAGCTGTTAGAAGCCGTCAACAA; GCAGTTGCACCAGAAGTTGACTGAAACTCAGGGAGAGCTGAAGGACCTGACCCAGAAGGTAGAGCTGCTG; GAGAAGTTTCGGGACAACTGTTTGGCAATTTTGGAGAGCAAGGGCCTTGATCCAGCTTTAGGCGGTGAGA; CCCTGGCATCACGACAAGAATCCACTACTGATCACATGGACTCTATGTTGCTGTTAGAAACTTTGCAAGA; GGAGCTGAAGCTTTTTAACGAAACAGCCAAAAAGCAGATGGAGGAGTTACAGGCCTTAAAGGTAAAGCTG; GAGATGAAAGAGGAAAGAGTCCGATTCCTAGAACAGCAAACCTTATGTAACAATCAAGTAAATGATTTAA; CAACAGCCCTTAAGGAAATGGAGCAGCTATTAGAAATG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222981 protein sequence; Red=Cloning site Green=Tags(s); ; MAAPEAPPLDRVFRTTWLSTECDSHPLPPSYRKFLFETQEADLAGGTTVAAGNLLNESEKDCGQDRRAPG; VQPCLLVTMTSVVKTVYSLQPSSALSGGQPADTQTRATSKSLLPVRSKEVDVSKQLHSGGPENDVTKITK; LRRENGQMKATDTATRRNVRKGYKPLSKQKSEEELKDKNQLLEAVNKQLHQKLTETQGELKDLTQKVELL; EKFRDNCLAILESKGLDPALGGETLASRQESTTDHMDSMLLLETLQEELKLFNETAKKQMEELQALKVKL; EMKEERVRFLEQQTLCNNQVNDLTTALKEMEQLLEM; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033286
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033286.2, NP_150628.2
• RefSeq Size: 1763 bp
• RefSeq ORF: 951 bp
• Locus ID: 90417
• UniProt ID: Q9Y448
• Cytogenetics: 15q15.1
• MW: 35.4 kDa
• Summary: Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase (PubMed:19667759). Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:19667759, PubMed:22110139). The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments (PubMed:21402792). Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division (PubMed:23035123). May be involved in UV-induced apoptosis via its interaction with PRPF19; however, these results need additional evidences (PubMed:24718257).[UniProtKB/Swiss-Prot Function]