BOK (NM_032515) Human Tagged ORF Clone
BOK (Myc-DDK-tagged)-Human BCL2-related ovarian killer (BOK)
|Product Name||BOK (NM_032515) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC222963 representing NM_032515
Red=Cloning site Blue=ORF Green=Tags(s)
>RC222963 representing NM_032515
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||636 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_032515.1, NM_032515.2, NM_032515.3, NM_032515.4, NP_115904.1|
|RefSeq Size||2617 bp|
|RefSeq ORF||639 bp|
|Protein Families||Druggable Genome|
|Gene Summary||The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq, Sep 2011]|
|The use of this cDNA Clones has been cited in the following citations:|
Role of B-Cell Lymphoma 2 Ovarian Killer (BOK) in Acute Toxicity of Human Lung Epithelial Cells Caused by Cadmium Chloride
,Zhang, F;Ren, L;Zhou, S;Duan, P;Xue, J;Chen, H;Feng, Y;Yue, X;Yuan, P;Liu, Q;Yang, P;Lei, Y;,
Med. Sci. Monit. 2019
,PubMed ID 31323016
|cDNA Clone Resources|
|SC104435||BOK (untagged)-Human BCL2-related ovarian killer (BOK)||
|RG222963||BOK (GFP-tagged) - Human BCL2-related ovarian killer (BOK)||
|RC222963L1||Lenti ORF clone of Human BCL2-related ovarian killer (BOK), Myc-DDK-tagged||
|RC222963L2||Lenti ORF clone of Human BCL2-related ovarian killer (BOK), mGFP tagged||
|RC222963L3||Lenti ORF clone of Human BCL2-related ovarian killer (BOK), Myc-DDK-tagged||
|RC222963L4||Lenti ORF clone of Human BCL2-related ovarian killer (BOK), mGFP tagged||