ATP11C (NM_173694) Human Tagged ORF Clone

CAT#: RC222893

ATP11C (Myc-DDK-tagged)-Human ATPase, class VI, type 11C (ATP11C), transcript variant 1

Product Images

Circular map for RC222893

• 

  Western blot validation of overexpression lysate (Cat# LY406564) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222893 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP11C
• Synonyms: ATPIG; ATPIQ; HACXL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222893 representing NM_173694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGGTCCCATCTCTCCCTCCAGCCTCTGAGTGTGCTGGAGAAGAGAAACGAGTTGGCACACGCA
  CAGTGTTTGTTGGCAATCATCCAGTTTCGGAAACAGAAGCTTACATTGCACAAAGATTTTGTGATAATAG
  AATAGTCTCATCTAAGTATACACTTTGGAATTTTCTCCCAAAGAATCTGTTTGAACAGTTTAGAAGAATT
  GCAAATTTTTATTTTCTCATAATCTTCCTTGTACAGGTCACAGTAGACACACCAACTAGCCCAGTTACCA
  GTGGACTTCCACTTTTCTTTGTTATAACTGTTACAGCCATCAAGCAGGGATATGAGGATTGGCTGAGACA
  CAGAGCTGACAATGAAGTCAACAAAAGCACTGTTTACATTATTGAAAATGCAAAGCGAGTGAGAAAAGAA
  AGTGAAAAAATCAAGGTTGGTGATGTAGTAGAAGTACAGGCAGATGAAACCTTTCCCTGTGATCTTATTC
  TTCTATCATCTTGCACCACTGATGGAACCTGTTATGTCACTACAGCCAGTCTTGATGGGGAATCCAATTG
  CAAGACACATTATGCAGTACGTGATACCATTGCACTGTGTACAGCAGAATCCATCGATACCCTCCGAGCA
  GCAATTGAATGTGAACAGCCTCAACCTGACCTCTACAAATTTGTTGGGCGAATCAATATCTACAGTAATA
  GTCTTGAGGCTGTTGCCAGGTCTTTGGGACCTGAAAATCTCTTGCTGAAAGGAGCTACGCTAAAAAATAC
  CGAGAAGATATATGGAGTTGCTGTTTACACTGGAATGGAAACCAAAATGGCTTTGAACTACCAAGGGAAA
  TCTCAGAAACGTTCTGCTGTTGAAAAATCTATTAATGCTTTCCTGATTGTATATTTATTTATCTTACTGA
  CCAAAGCTGCAGTATGCACTACTCTAAAGTATGTTTGGCAAAGTACCCCATACAATGATGAACCTTGGTA
  TAACCAAAAGACTCAGAAAGAGCGAGAGACCTTGAAGGTTTTAAAAATGTTCACCGACTTCCTATCATTT
  ATGGTTCTATTCAACTTTATCATTCCTGTCTCCATGTACGTCACAGTAGAAATGCAGAAATTCTTGGGCT
  CCTTCTTCATCTCATGGGATAAGGACTTTTATGATGAAGAAATTAATGAAGGAGCCCTGGTTAACACATC
  AGACCTTAATGAAGAACTTGGTCAGGTGGATTATGTATTTACAGATAAGACTGGAACACTCACTGAAAAC
  AGCATGGAATTCATTGAATGCTGCATAGATGGCCACAAATATAAAGGTGTAACTCAAGAGGTTGATGGAT
  TATCTCAAACTGATGGAACTTTAACATATTTTGACAAAGTAGATAAGAATCGAGAAGAGCTGTTTCTACG
  TGCCTTGTGTTTATGTCATACTGTAGAAATCAAAACAAACGATGCTGTTGATGGAGCTACAGAATCAGCT
  GAATTAACCTATATCTCCTCTTCACCAGATGAAATAGCTTTGGTGAAAGGAGCTAAAAGGTACGGGTTCA
  CATTTTTAGGAAATCGAAATGGATATATGAGAGTAGAGAACCAAAGAAAAGAAATAGAAGAATATGAACT
  TCTTCACACCTTAAACTTTGATGCTGTCCGGCGACGTATGAGTGTAATTGTGAAGACTCAAGAAGGAGAC
  ATACTTCTCTTTTGTAAAGGAGCAGACTCGGCAGTTTTTCCCAGAGTGCAAAATCATGAAATTGAGTTAA
  CTAAAGTCCATGTGGAACGTAATGCAATGGATGGGTATCGGACACTCTGTGTAGCCTTCAAAGAAATTGC
  TCCAGATGATTATGAAAGAATTAACAGACAGCTCATAGAGGCAAAAATGGCCTTACAAGACAGAGAAGAA
  AAAATGGAAAAAGTTTTCGATGATATTGAGACAAACATGAATTTAATTGGAGCCACTGCAGTTGAAGACA
  AGCTACAAGATCAAGCTGCAGAGACCATTGAAGCTCTGCATGCAGCAGGCCTGAAAGTCTGGGTGCTCAC
  TGGGGACAAGATGGAGACAGCTAAATCCACATGCTATGCCTGCCGCCTTTTCCAGACCAACACTGAGCTC
  TTAGAACTAACCACAAAAACCATTGAAGAAAGTGAAAGGAAAGAAGATCGATTACATGAATTATTGATAG
  AATATCGCAAGAAATTGCTGCATGAGTTTCCTAAAAGTACTAGAAGCTTTAAAAAAGCATGGACAGAACA
  TCAGGAATATGGATTAATCATAGATGGCTCCACATTGTCACTCATACTAAATTCTAGTCAAGACTCTAGT
  TCAAACAATTACAAAAGCATTTTCCTACAAATATGTATGAAGTGTACTGCAGTGCTCTGCTGTCGGATGG
  CACCATTACAGAAAGCCCAGATTGTCAGAATGGTGAAGAATTTAAAAGGCAGCCCAATAACTCTGTCGAT
  AGGTGATGGTGCCAATGATGTTAGTATGATCTTGGAATCCCATGTGGGAATAGGTATTAAAGGCAAAGAA
  GGTCGCCAAGCAGCTAGGAATAGCGATTATTCTGTTCCAAAGTTTAAACACTTAAAGAAACTGCTGTTGG
  CTCATGGACATCTATATTATGTGAGAATAGCACACCTTGTACAGTACTTCTTCTATAAGAACCTTTGTTT
  CATTTTGCCACAGTTTTTGTACCAGTTCTTCTGTGGATTCTCACAACAGCCACTGTATGATGCTGCTTAC
  CTTACAATGTACAATATCTGCTTCACATCCTTGCCCATCCTGGCCTATAGTCTACTGGAACAGCACATCA
  ACATTGACACTCTGACCTCAGATCCCCGATTGTATATGAAAATTTCTGGCAATGCCATGCTACAGTTGGG
  CCCCTTCTTATATTGGACATTTCTGGCTGCCTTTGAAGGGACAGTGTTCTTCTTTGGGACTTACTTTCTT
  TTTCAGACTGCATCCCTAGAAGAAAATGGAAAGGTATACGGAAACTGGACTTTTGGAACCATTGTTTTTA
  CAGTCTTAGTATTCACTGTAACCCTGAAGCTTGCCTTGGATACCCGATTCTGGACGTGGATAAATCACTT
  TGTGATTTGGGGTTCTTTAGCCTTCTATGTATTTTTCTCATTCTTCTGGGGAGGAATTATTTGGCCTTTT
  CTCAAGCAACAGAGAATGTATTTTGTATTTGCCCAAATGCTGTCTTCTGTATCCACATGGTTGGCTATAA
  TTCTTCTAATATTTATCAGCCTGTTCCCTGAGATTCTTCTGATAGTATTAAAGAATGTAAGAAGAAGAAG
  TGCCAGGAGAAATCTGAGCTGTAGAAGGGCATCTGACTCATTATCCGCCAGACCTTCAGTCAGACCTCTT
  CTTTTACGAACATTCTCAGACGAATCTAATGTATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222893 representing NM_173694
  Red=Cloning site Green=Tags(s)
  
  MQMVPSLPPASECAGEEKRVGTRTVFVGNHPVSETEAYIAQRFCDNRIVSSKYTLWNFLPKNLFEQFRRI
  ANFYFLIIFLVQVTVDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHRADNEVNKSTVYIIENAKRVRKE
  SEKIKVGDVVEVQADETFPCDLILLSSCTTDGTCYVTTASLDGESNCKTHYAVRDTIALCTAESIDTLRA
  AIECEQPQPDLYKFVGRINIYSNSLEAVARSLGPENLLLKGATLKNTEKIYGVAVYTGMETKMALNYQGK
  SQKRSAVEKSINAFLIVYLFILLTKAAVCTTLKYVWQSTPYNDEPWYNQKTQKERETLKVLKMFTDFLSF
  MVLFNFIIPVSMYVTVEMQKFLGSFFISWDKDFYDEEINEGALVNTSDLNEELGQVDYVFTDKTGTLTEN
  SMEFIECCIDGHKYKGVTQEVDGLSQTDGTLTYFDKVDKNREELFLRALCLCHTVEIKTNDAVDGATESA
  ELTYISSSPDEIALVKGAKRYGFTFLGNRNGYMRVENQRKEIEEYELLHTLNFDAVRRRMSVIVKTQEGD
  ILLFCKGADSAVFPRVQNHEIELTKVHVERNAMDGYRTLCVAFKEIAPDDYERINRQLIEAKMALQDREE
  KMEKVFDDIETNMNLIGATAVEDKLQDQAAETIEALHAAGLKVWVLTGDKMETAKSTCYACRLFQTNTEL
  LELTTKTIEESERKEDRLHELLIEYRKKLLHEFPKSTRSFKKAWTEHQEYGLIIDGSTLSLILNSSQDSS
  SNNYKSIFLQICMKCTAVLCCRMAPLQKAQIVRMVKNLKGSPITLSIGDGANDVSMILESHVGIGIKGKE
  GRQAARNSDYSVPKFKHLKKLLLAHGHLYYVRIAHLVQYFFYKNLCFILPQFLYQFFCGFSQQPLYDAAY
  LTMYNICFTSLPILAYSLLEQHINIDTLTSDPRLYMKISGNAMLQLGPFLYWTFLAAFEGTVFFFGTYFL
  FQTASLEENGKVYGNWTFGTIVFTVLVFTVTLKLALDTRFWTWINHFVIWGSLAFYVFFSFFWGGIIWPF
  LKQQRMYFVFAQMLSSVSTWLAIILLIFISLFPEILLIVLKNVRRRSARRNLSCRRASDSLSARPSVRPL
  LLRTFSDESNVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173694
• ORF Size: 3396 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173694.4, NP_775965.2
• RefSeq Size: 6135 bp
• RefSeq ORF: 3399 bp
• Locus ID: 286410
• UniProt ID: Q8NB49
• Cytogenetics: Xq27.1
• Protein Families: Transmembrane
• MW: 129.4 kDa
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. In the cell membrane of erythrocytes, it is required to maintain phosphatidylserine (PS) in the inner leaflet preventing its exposure on the surface. This asymmetric distribution is critical for the survival of erythrocytes in circulation since externalized PS is a phagocytic signal for splenic macrophages (PubMed:26944472). Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity). Required for B cell differentiation past the pro-B cell stage (By similarity). Seems to mediate PS flipping in pro-B cells (By similarity). May be involved in the transport of cholestatic bile acids (By similarity).[UniProtKB/Swiss-Prot Function]