PWWP2A (NM_052927) Human Tagged ORF Clone

CAT#: RC222777

PWWP2A (Myc-DDK-tagged)-Human PWWP domain containing 2A (PWWP2A), transcript variant 1

Product Images

Circular map for RC222777

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PWWP2A
• Synonyms: MST101
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222777 representing NM_052927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTGGCTGCAGAGGCGGCAGCGACTGCAGCGTCCCCCGGGGAGGGGGGCGCCGGCGAGGCCG
  AGCCGGAGATGGAGCCCATCCCCGGCAGTGAGGCCGGCACTGACCCCCTCCCGGTCACGGCCACTGAAGC
  GTCTGTGCCGGATGGCGAGACTGACGGGCAGCAATCCGCTCCTCAGGCCGACGAGCCGCCGCTCCCGCCG
  CCACCGCCGCCGCCGGGGGAGCTCGCCCGCAGCCCAGAGGCGGTGGGGCCGGAGCTGGAGGCTGAGGAGA
  AACTGTCCGTTCGGGTGGCGGAGTCGGCGGCAGCCGCGCCTCAGGGAGGGCCGGAACTTCCACCTTCTCC
  TGCATCGCCGCCGGAGCAGCCCCCGGCTCCCGAGGAGCGCGAGGAGCCGCCGCTGCCTCAGCCCGTAGCC
  CCGGCGCTCGTGCCGCCGGCGGGCGGGGACTCCACGGTGTCGCAACTGATCCCGGGCTCGGAGGTGCGGG
  TCACGCTGGACCACATCATTGAGGACGCGCTTGTCGTGTCGTTCCGCTTCGGGGAGAAGCTCTTCTCCGG
  GGTCCTCATGGATCTGTCCAAAAGGTTTGGGCCCCATGGTATCCCTGTGACAGTATTTCCCAAAAGGGAA
  TATAAGGATAAACCAGAAGCCATGCCGCTCCAAAGTAATACATTCCAAGAAGGGACAGAAGTCAAGTGTG
  AAGCAAATGGTGCTGTTCCCGATGACCCTTCTCCTGTCCCGCATCCCGAGCTGAGCTTGGCTGAAAGCCT
  GTGGACTTCCAAACCACCACCTCTCTTCCATGAAGGAGCACCTTATCCTCCCCCTTTGTTTATCAGGGAC
  ACATATAACCAATCAATACCTCAGCCACCTCCTCGGAAAATTAAGCGACCCAAACGAAAAATGTACAGGG
  AAGAACCCACTTCAATAATGAATGCTATTAAACTACGACCCAGGCAAGTTCTGTGTGATAAATGTAAAAA
  CAGTGTTGTTGCTGAAAAAAAGGAAATTAGAAAAGGTAGTAGTGCAACTGACTCTTCTAAATATGAAGAT
  AAAAAACGGAGAAATGAAAGTGTAACTACTGTGAACAAAAAACTGAAAACTGACCATAAAGTGGATGGGA
  AAAACCAAAATGAAAGCCAGAAAAGAAATGCTGTGGTTAAGGTTTCAAATATTGCTCACAGCAGAGGCAG
  AGTAGTAAAAGTTTCTGCTCAGGCAAATACATCAAAAGCTCAGTTAAGTACTAAAAAAGTTCTCCAGAGT
  AAGAACATGGATCATGCGAAAGCTCGGGAAGTGTTAAAAATTGCCAAAGAAAAGGCACAAAAGAAGCAAA
  ATGAAACCTCTACTTCCAAAAATGCACATTCAAAAGTCCATTTCACACGTCGATATCAGAATCCTAGCTC
  AGGTTCCCTTCCACCCCGGGTTCGTTTAAAACCACAGAGGTACAGGAATGAAGAAAATGACTCTTCTCTG
  AAGACAGGACTTGAGAAAATGCGGAGTGGCAAGATGGCACCCAAGCCCCAGTCTCGCTGCACCTCTACCC
  GCTCAGCAGGTCTCAACAAATGGCAGCTATTACATCAGACAGTGACGAGTCCTGCTGCTCCCTTACAGTG
  TCTGACAGACCACTGTGGATTCAGACTGGGAGCATTGAAGTTAACAGTGAAACGGGCAGCACAAAGACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222777 representing NM_052927
  Red=Cloning site Green=Tags(s)
  
  MAAVAAEAAATAASPGEGGAGEAEPEMEPIPGSEAGTDPLPVTATEASVPDGETDGQQSAPQADEPPLPP
  PPPPPGELARSPEAVGPELEAEEKLSVRVAESAAAAPQGGPELPPSPASPPEQPPAPEEREEPPLPQPVA
  PALVPPAGGDSTVSQLIPGSEVRVTLDHIIEDALVVSFRFGEKLFSGVLMDLSKRFGPHGIPVTVFPKRE
  YKDKPEAMPLQSNTFQEGTEVKCEANGAVPDDPSPVPHPELSLAESLWTSKPPPLFHEGAPYPPPLFIRD
  TYNQSIPQPPPRKIKRPKRKMYREEPTSIMNAIKLRPRQVLCDKCKNSVVAEKKEIRKGSSATDSSKYED
  KKRRNESVTTVNKKLKTDHKVDGKNQNESQKRNAVVKVSNIAHSRGRVVKVSAQANTSKAQLSTKKVLQS
  KNMDHAKAREVLKIAKEKAQKKQNETSTSKNAHSKVHFTRRYQNPSSGSLPPRVRLKPQRYRNEENDSSL
  KTGLEKMRSGKMAPKPQSRCTSTRSAGLNKWQLLHQTVTSPAAPLQCLTDHCGFRLGALKLTVKRAAQRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052927
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052927.4
• RefSeq Size: 4013 bp
• RefSeq ORF: 1683 bp
• Locus ID: 114825
• UniProt ID: Q96N64
• Cytogenetics: 5q33.3
• MW: 60.5 kDa
• Gene Summary: H2A.Z-specific chromatin binding protein which may play an important role in the neural crest stem cell migration and differentiation during early development. Also required for proper mitosis progression.[UniProtKB/Swiss-Prot Function]