CTNND1 (NM_001085458) Human Tagged ORF Clone

CAT#: RC222771

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CTNND1 (Myc-DDK-tagged)-Human catenin (cadherin-associated protein), delta 1 (CTNND1), transcript variant 1



  "NM_001085458" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,168.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CTNND1
Synonyms BCDS2; CAS; CTNND; p120; p120(CAS); p120(CTN); P120CAS; P120CTN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222771 representing NM_001085458
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACTCAGAGGTGGAGTCGACCGCCAGCATCTTGGCCTCTGTGAAGGAACAAGAGGCCCAGTTTG
AGAAGCTGACCCGGGCGCTGGAGGAGGAACGGCGCCACGTCTCGGCGCAGCTGGAACGCGTCCGGGTCTC
ACCACAAGATGCCAACCCACTCATGGCCAACGGCACACTCACCCGCCGGCATCAGAACGGCCGGTTTGTG
GGCGATGCTGACCTTGAAAGACAGAAATTTTCAGATTTGAAACTCAACGGACCCCAGGATCACAGTCACC
TTCTATATAGCACCATCCCCAGGATGCAGGAGCCGGGGCAGATTGTGGAGACCTACACGGAGGAGGATCC
TGAGGGAGCCATGTCTGTAGTCTCTGTGGAGACCTCAGATGATGGGACCACTCGGCGCACAGAGACCACG
GTCAAGAAAGTAGTGAAGACTGTGACAACACGGACAGTACAGCCAGTCGCTATGGGACCAGACGGGTTGC
CTGTGGATGCTTCATCAGTTTCTAACAACTATATCCAGACTTTGGGTCGTGATTTCCGCAAGAATGGCAA
TGGGGGACCTGGTCCCTATGTGGGGCAAGCTGGCACTGCTACCCTTCCTAGGAACTTCCACTACCCTCCT
GATGGTTATAGTCGCCACTATGAAGATGGTTATCCAGGTGGCAGTGATAACTATGGCAGTCTGTCCCGGG
TGACCCGCATTGAGGAGCGGTATAGGCCCAGCATGGAAGGCTACCGGGCACCTAGTAGACAGGATGTGTA
TGGGCCCCAACCCCAGGTTCGGGTAGGTGGGAGCAGCGTGGATCTGCATCGCTTTCATCCAGAGCCTTAT
GGGCTAGAGGATGACCAGCGTAGTATGGGCTATGATGACCTGGATTATGGTATGATGTCTGATTATGGCA
CTGCCCGTCGGACTGGGACACCCTCTGACCCTCGTCGGCGCCTCAGGAGCTATGAAGACATGATTGGTGA
GGAGGTGCCATCGGATCAATACTACTGGGCTCCTTTGGCCCAGCATGAGCGAGGAAGTTTAGCAAGCTTG
GATAGCCTGCGCAAAGGAGGGCCTCCACCTCCTAATTGGAGACAGCCAGAGCTGCCAGAGGTGATCGCCA
TGCTTGGATTCCGCTTGGATGCTGTCAAGTCCAATGCAGCTGCATACCTGCAACACTTATGCTACCGCAA
TGACAAGGTGAAGACTGACGTGCGGAAGCTCAAGGGCATCCCAGTACTGGTGGGATTGTTAGACCATCCC
AAAAAGGAAGTGCACCTTGGAGCCTGTGGAGCTCTCAAGAATATCTCTTTTGGACGTGACCAGGATAACA
AGATTGCCATAAAAAACTGTGATGGTGTGCCTGCCCTTGTGCGATTGCTTCGAAAGGCTCGTGATATGGA
CCTTACTGAAGTTATTACCGGAACCCTGTGGAATCTTTCATCCCATGACTCAATCAAAATGGAGATTGTG
GACCATGCACTGCATGCCTTGACAGATGAAGTGATCATTCCTCATTCTGGTTGGGAGCGGGAACCTAATG
AAGACTGTAAGCCACGCCATATTGAGTGGGAATCGGTGCTCACCAACACAGCTGGCTGCCTTAGGAATGT
AAGCTCAGAGAGGAGTGAAGCTCGCCGGAAACTTCGGGAATGTGATGGTTTAGTTGATGCCCTCATTTTC
ATTGTTCAGGCTGAGATTGGGCAGAAGGATTCAGACAGCAAGCTTGTAGAGAACTGTGTTTGCCTTCTTC
GGAACTTATCATATCAAGTTCACCGGGAGATCCCACAGGCAGAGCGTTACCAAGAGGCAGCTCCCAATGT
TGCCAACAATACTGGGCCACATGCTGCCAGTTGCTTTGGGGCCAAGAAGGGCAAAGATGAGTGGTTCTCC
AGAGGGAAAAAACCTATAGAGGATCCAGCAAACGATACAGTGGATTTCCCTAAAAGAACGAGTCCAGCTC
GAGGCTATGAGCTCTTATTTCAGCCAGAGGTGGTTCGGATATACATCTCACTTCTTAAGGAGAGCAAGAC
TCCTGCCATCCTAGAAGCCTCAGCTGGAGCTATCCAGAACTTGTGTGCTGGGCGCTGGACGTATGGTCGA
TACATCCGCTCTGCTCTGCGTCAAGAGAAGGCTCTTTCTGCCATAGCTGACCTCCTGACTAATGAACATG
AACGGGTGGTGAAAGCTGCATCTGGAGCACTGAGAAACCTGGCTGTGGATGCTCGCAACAAAGAATTAAT
TGGTAAACATGCTATTCCTAACTTGGTAAAGAATCTGCCAGGAGGACAGCAGAACTCCTCTTGGAATTTC
TCTGAGGACACTGTCATCTCTATTTTGAACACTATCAACGAGGTTATCGCTGAGAACTTGGAGGCTGCCA
AAAAGCTTCGAGAGACACAGGGTATTGAGAAGCTGGTGTTGATCAACAAATCAGGGAACCGCTCAGAAAA
AGAAGTTCGAGCAGCAGCACTTGTATTACAGACAATCTGGGGATATAAGGAACTGCGGAAGCCACTGGAA
AAAGAAGGATGGAAGAAATCAGACTTTCAGGTGAATCTAAACAATGCTTCCCGAAGCCAGAGCAGTCATT
CATATGATGATAGTACTCTCCCTCTCATTGACCGGAACCAAAAATCAGATAAGAAACCTGATCGGGAAGA
AATTCAGATGAGCAATATGGGATCAAACACAAAATCACTAGATAACAACTATTCCACACCAAATGAGAGA
GGAGACCACAATAGAACACTGGATCGATCGGGGGATCTAGGCGACATGGAGCCATTGAAGGGAACAACAC
CCTTGATGCAGGACGAGGGGCAGGAATCTCTGGAGGAAGAGTTGGATGTGTTGGTTTTGGATGATGAGGG
GGGCCAAGTGTCTTACCCCTCCATGCAGAAGATT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC222771 representing NM_001085458
Red=Cloning site Green=Tags(s)

MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFV
GDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETT
VKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPP
DGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPY
GLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASL
DSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHP
KKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIV
DHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIF
IVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFS
RGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGR
YIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNF
SEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLE
KEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNER
GDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001085458
ORF Size 2904 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001085458.1, NP_001078927.1
RefSeq Size 6363 bp
RefSeq ORF 2907 bp
Locus ID 1500
UniProt ID O60716
Cytogenetics 11q12.1
Protein Families Druggable Genome
Protein Pathways Adherens junction, Leukocyte transendothelial migration
MW 108.2 kDa
Gene Summary This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

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