XPNPEP2 (NM_003399) Human Tagged ORF Clone

CAT#: RC222768

XPNPEP2 (Myc-DDK-tagged)-Human X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound (XPNPEP2)

Product Images

Circular map for RC222768

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  Western blot validation of overexpression lysate (Cat# LY418716) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222768 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified XPNPEP2 protein (Cat# TP322768). The protein was produced from HEK293T cells transfected with XPNPEP2 cDNA clone (Cat# RC222768) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XPNPEP2
• Synonyms: AEACEI; APP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222768 representing NM_003399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGCTCACTGGGGCTGCTGCCCCTGGCTGGTCCTCCTCTGTGCTTGTGCCTGGGGCCACACAA
  AGCCAGTGGACCTTGGAGGGCAGGATGTGAGAAACTGTTCCACCAACCCCCCTTACCTTCCAGTTACTGT
  GGTCAATACCACAATGTCACTCACAGCCCTCCGCCAGCAGATGCAGACCCAGAATCTCTCAGCCTACATC
  ATCCCAGGCACAGATGCTCACATGAACGAGTACATCGGCCAACATGACGAGAGGCGTGCGTGGATTACAG
  GCTTTACAGGGTCTGCAGGAACTGCAGTGGTGACTATGAAGAAAGCAGCTGTCTGGACCGACAGTCGCTA
  CTGGACTCAGGCTGAGCGGCAGATGGACTGCAACTGGGAGCTCCATAAGGAAGTTGGCACCACTCCTATT
  GTCACCTGGCTCCTCACCGAGATTCCCGCTGGAGGGCGTGTGGGTTTTGACCCCTTCCTCTTGTCCATTG
  ACACCTGGGAGAGTTATGATCTGGCCCTCCAAGGCTCTAACAGACAGCTGGTGTCCATCACAACCAATCT
  TGTGGACCTGGTATGGGGATCAGAGAGGCCACCGGTTCCAAATCAACCCATTTATGCCCTGCAGGAGGCA
  TTCACAGGGAGCACTTGGCAGGAGAAAGTATCTGGCGTCCGAAGCCAGATGCAGAAGCATCAAAAGGTCC
  CGACTGCCGTCCTTCTGTCGGCGCTTGAGGAGACGGCCTGGCTCTTCAACCTTCGAGCCAGTGACATCCC
  CTATAACCCCTTCTTCTATTCCTACACGCTGCTCACAGACTCTTCTATTAGGTTGTTTGCAAACAAGAGT
  CGCTTTAGCTCCGAAACCTTGAGCTATCTGAACTCCAGTTGCACAGGCCCCATGTGTGTGCAAATCGAGG
  ATTACAGCCAAGTTCGTGACAGCATCCAGGCCTACTCATTGGGAGATGTGAGGATCTGGATTGGGACCAG
  CTATACCATGTATGGGATCTATGAAATGATACCCAAGGAGAAACTCGTGACAGACACCTACTCCCCAGTG
  ATGATGACCAAGGCAGTGAAGAACAGCAAGGAGCAGGCCCTCCTCAAGGCCAGCCACGTGCGGGACGCTG
  TGGCTGTGATCCGGTACTTGGTCTGGCTGGAGAAGAACGTGCCCAAAGGCACAGTGGATGAGTTCTCGGG
  GGCAGAGATCGTGGACAAGTTCCGAGGAGAAGAACAGTTCTCCTCCGGACCCAGTTTTGAAACCATCTCT
  GCTAGTGGTCTGAATGCTGCCCTGGCCCACTACAGCCCGACCAAGGAGCTGAACCGCAAGCTGTCCTCAG
  ATGAGATGTACCTGCTGGACTCTGGGGGGCAGTACTGGGACGGGACCACAGACATCACCAGAACAGTCCA
  CTGGGGCACCCCCTCTGCCTTCCAGAAGGAGGCATACACCCGTGTGCTGATAGGAAATATTGACCTGTCC
  AGGCTCATCTTTCCCGCTGCTACATCAGGGCGAATGGTGGAGGCCTTTGCCCGCAGAGCCTTGTGGGATG
  CTGGTCTCAATTATGGTCATGGGACAGGCCACGGCATTGGCAACTTCCTGTGTGTGCATGAGTGGCCAGT
  GGGATTCCAGTCCAACAACATCGCTATGGCCAAGGGCATGTTCACTTCCATTGAACCTGGTTACTATAAG
  GATGGAGAATTTGGGATCCGTCTCGAAGATGTGGCTCTCGTGGTAGAAGCAAAGACCAAGTACCCAGGGA
  GCTACCTGACCTTTGAAGTGGTATCATTTGTGCCCTATGACCGGAACCTCATCGATGTCAGCCTGCTGTC
  TCCCGAGCATCTCCAGTACCTGAATCGCTACTACCAGACCATCCGGGAGAAGGTGGGTCCAGAGCTGCAG
  AGGCGCCAGCTACTAGAGGAGTTCGAGTGGCTTCAACAGCACACAGAGCCCCTGGCCGCCAGGGCCCCAG
  ACACCGCCTCCTGGGCCTCTGTGTTAGTGGTCTCCACCCTTGCCATCCTTGGCTGGAGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222768 representing NM_003399
  Red=Cloning site Green=Tags(s)
  
  MARAHWGCCPWLVLLCACAWGHTKPVDLGGQDVRNCSTNPPYLPVTVVNTTMSLTALRQQMQTQNLSAYI
  IPGTDAHMNEYIGQHDERRAWITGFTGSAGTAVVTMKKAAVWTDSRYWTQAERQMDCNWELHKEVGTTPI
  VTWLLTEIPAGGRVGFDPFLLSIDTWESYDLALQGSNRQLVSITTNLVDLVWGSERPPVPNQPIYALQEA
  FTGSTWQEKVSGVRSQMQKHQKVPTAVLLSALEETAWLFNLRASDIPYNPFFYSYTLLTDSSIRLFANKS
  RFSSETLSYLNSSCTGPMCVQIEDYSQVRDSIQAYSLGDVRIWIGTSYTMYGIYEMIPKEKLVTDTYSPV
  MMTKAVKNSKEQALLKASHVRDAVAVIRYLVWLEKNVPKGTVDEFSGAEIVDKFRGEEQFSSGPSFETIS
  ASGLNAALAHYSPTKELNRKLSSDEMYLLDSGGQYWDGTTDITRTVHWGTPSAFQKEAYTRVLIGNIDLS
  RLIFPAATSGRMVEAFARRALWDAGLNYGHGTGHGIGNFLCVHEWPVGFQSNNIAMAKGMFTSIEPGYYK
  DGEFGIRLEDVALVVEAKTKYPGSYLTFEVVSFVPYDRNLIDVSLLSPEHLQYLNRYYQTIREKVGPELQ
  RRQLLEEFEWLQQHTEPLAARAPDTASWASVLVVSTLAILGWSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003399
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003399.6
• RefSeq Size: 3329 bp
• RefSeq ORF: 2025 bp
• Locus ID: 7512
• UniProt ID: O43895
• Cytogenetics: Xq26.1
• Protein Families: Druggable Genome, Protease
• MW: 75.4 kDa
• Gene Summary: Aminopeptidase P is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. Structurally, the enzyme is a member of the 'pita bread fold' family and occurs in mammalian tissues in both soluble and GPI-anchored membrane-bound forms. A membrane-bound and soluble form of this enzyme have been identified as products of two separate genes. [provided by RefSeq, Jul 2008]