C10orf118 (CCDC186) (NM_018017) Human Tagged ORF Clone

CAT#: RC222741

C10orf118 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 118 (C10orf118)

Product Images

Circular map for RC222741

• 

  Western blot validation of overexpression lysate (Cat# LY413382) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222741 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C10orf118
• Synonyms: C10orf118; CCCP-1; golgin104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222741 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGACAGACCACATAGCCTCTACTTCCTCTGATAAAAATGTTGGGAAAACACCTGAATTAAAGG
  AAGACTCATGCAACTTGTTTTCTGGCAATGAAAGCAGCAAATTAGAAAATGAGTCCAAACTATTGTCATT
  AAACACTGATAAAACTTTATGTCAACCTAATGAGCATAATAATCGAATTGAAGCCCAGGAAAATTATATT
  CCAGATCATGGTGGAGGTGAGGATTCTTGTGCCAAAACAGACACAGGCTCAGAAAATTCTGAACAAATAG
  CTAATTTTCCTAGTGGAAATTTTGCTAAACATATTTCAAAAACAAATGAAACAGAACAGAAAGTAACACA
  AATATTGGTGGAATTAAGGTCATCTACATTTCCAGAATCAGCTAATGAAAAGACTTATTCAGAAAGCCCC
  TATGATACAGACTGCACCAAGAAATTTATTTCAAAAATAAAGAGCGTTTCAGCATCAGAGGATTTGTTGG
  AAGAAATAGAATCTGAGCTCTTATCTACGGAGTTTGCAGAACATCGAGTACCAAATGGAATGAATAAGGG
  AGAACATGCATTAGTTCTGTTTGAAAAGTGTGTGCAAGATAAATATTTGCAGCAGGAACATATCATAAAA
  AAGTTAATTAAAGAAAATAAGAAGCATCAGGAGCTCTTCGTAGACATTTGTTCAGAAAAAGACAATTTAA
  GAGAAGAACTAAAGAAAAGAACAGAAACTGAGAAGCAGCATATGAACACAATTAAACAGTTAGAATCAAG
  AATAGAAGAACTTAATAAAGAAGTTAAAGCTTCCAGAGATCAACTAATAGCTCAAGACGTTACAGCTAAA
  AATGCAGTTCAGCAGTTACACAAAGAGATGGCCCAACGGATGGAACAGGCCAACAAGAAATGTGAAGAGG
  CACGCCAAGAAAAAGAAGCAATGGTAATGAAATATGTAAGAGGTGAGAAGGAATCTTTAGATCTTCGAAA
  GGAAAAAGAGACACTTGAGAAAAAACTTAGAGATGCAAATAAGGAACTTGAGAAAAACACTAACAAAATT
  AAGCAGCTTTCTCAGGAGAAAGGACGGTTGCACCAGCTGTATGAAACTAAGGAAGGCGAAACGACTAGAC
  TCATCAGAGAAATAGACAAATTAAAGGAAGACATTAACTCTCACGTCATCAAAGTAAAGTGGGCACAAAA
  CAAATTAAAAGCTGAAATGGATTCACACAAGGAAACCAAAGATAAACTCAAAGAAACAACAACAAAATTA
  ACACAAGCAAAGGAAGAAGCAGATCAGATACGAAAAAACTGTCAGGATATGATAAAAACATATCAGGAGT
  CAGAAGAAATTAAATCAAATGAGCTTGATGCAAAGCTTAGAGTCACAAAAGGAGAACTTGAAAAACAAAT
  GCAAGAAAAATCTGACCAGCTAGAGATGCATCATGCCAAAATAAAGGAACTAGAAGATCTGAAGAGAACA
  TTTAAGGAGGGTATGGATGAGTTAAGAACACTGAGAACAAAGGTGAAATGTCTAGAAGATGAACGATTAA
  GAACAGAAGATGAATTATCAAAATATAAGGAAATTATTAATCGCCAAAAAGCTGAAATTCAGAATTTATT
  GGACAAGGTGAAAACTGCAGATCAGCTACAGGAGCAGCTTCAAAGAGGTAAGCAAGAAATTGAAAATTTG
  AAAGAAGAAGTGGAAAGTCTTAATTCTTTGATTAATGACCTACAAAAAGACATCGAAGGCAGTAGGAAAA
  GAGAATCTGAGCTGCTGCTGTTTACAGAAAGGCTCACTAGTAAGAATGCACAGCTTCAGTCTGAATCCAA
  TTCTTTGCAGTCACAATTTGATAAAGTTTCCTGTAGTGAAAGTCAGTTACAAAGCCAGTGTGAACAAATG
  AAACAGACAAATATTAATTTGGAAAGTAGGTTGTTGAAAGAGGAAGAACTGCGAAAAGAGGAAGTCCAAA
  CTCTGCAAGCTGAACTCGCTTGTAGACAAACAGAAGTTAAAGCATTGAGTACCCAGGTAGAAGAATTAAA
  AGATGAGTTAGTAACTCAGAGACGTAAACATGCCTCTAGTATCAAGGATCTCACCAAACAACTTCAGCAA
  GCACGAAGAAAATTAGATCAGGTTGAGAGTGGAAGCTATGACAAAGAAGTCAGCAGCATGGGAAGTCGTT
  CTAGTTCATCAGGGTCCCTGAATGCTCGAAGCAGTGCAGAAGATCGATCTCCAGAAAATACTGGGTCCTC
  AGTAGCTGTGGATAACTTTCCACAAGTAGATAAGGCCATGTTGATTGAGAGAATAGTTAGGCTGCAAAAA
  GCACATGCCCGGAAAAATGAAAAGATAGAATTTATGGAGGACCACATCAAACAACTGGTGGAAGAAATTA
  GGAAAAAAACAAAAATAATTCAAAGTTATATTTTACGAGAAGAATCAGGCACACTTTCTTCAGAGGCATC
  TGATTTTAACAAAGTTCATTTAAGTAGACGGGGTGGCATCATGGCATCTTTATATACATCCCATCCAGCT
  GACAATGGATTAACATTGGAGCTCTCTTTGGAAATCAACCGAAAATTACAGGCTGTTTTGGAGGATACGT
  TACTAAAAAATATTACTTTGAAGGAAAATCTACAAACACTTGGAACAGAAATAGAACGTCTTATTAAACA
  CCAGCATGAACTAGAACAGAGGACAAAGAAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222741 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSETDHIASTSSDKNVGKTPELKEDSCNLFSGNESSKLENESKLLSLNTDKTLCQPNEHNNRIEAQENYI
  PDHGGGEDSCAKTDTGSENSEQIANFPSGNFAKHISKTNETEQKVTQILVELRSSTFPESANEKTYSESP
  YDTDCTKKFISKIKSVSASEDLLEEIESELLSTEFAEHRVPNGMNKGEHALVLFEKCVQDKYLQQEHIIK
  KLIKENKKHQELFVDICSEKDNLREELKKRTETEKQHMNTIKQLESRIEELNKEVKASRDQLIAQDVTAK
  NAVQQLHKEMAQRMEQANKKCEEARQEKEAMVMKYVRGEKESLDLRKEKETLEKKLRDANKELEKNTNKI
  KQLSQEKGRLHQLYETKEGETTRLIREIDKLKEDINSHVIKVKWAQNKLKAEMDSHKETKDKLKETTTKL
  TQAKEEADQIRKNCQDMIKTYQESEEIKSNELDAKLRVTKGELEKQMQEKSDQLEMHHAKIKELEDLKRT
  FKEGMDELRTLRTKVKCLEDERLRTEDELSKYKEIINRQKAEIQNLLDKVKTADQLQEQLQRGKQEIENL
  KEEVESLNSLINDLQKDIEGSRKRESELLLFTERLTSKNAQLQSESNSLQSQFDKVSCSESQLQSQCEQM
  KQTNINLESRLLKEEELRKEEVQTLQAELACRQTEVKALSTQVEELKDELVTQRRKHASSIKDLTKQLQQ
  ARRKLDQVESGSYDKEVSSMGSRSSSSGSLNARSSAEDRSPENTGSSVAVDNFPQVDKAMLIERIVRLQK
  AHARKNEKIEFMEDHIKQLVEEIRKKTKIIQSYILREESGTLSSEASDFNKVHLSRRGGIMASLYTSHPA
  DNGLTLELSLEINRKLQAVLEDTLLKNITLKENLQTLGTEIERLIKHQHELEQRTKKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018017
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018017.4
• RefSeq Size: 6277 bp
• RefSeq ORF: 2697 bp
• Locus ID: 55088
• UniProt ID: Q7Z3E2
• Cytogenetics: 10q25.3
• MW: 103.7 kDa