Adenylate cyclase 1 (ADCY1) (NM_021116) Human Tagged ORF Clone

CAT#: RC222738

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ADCY1 (Myc-DDK-tagged)-Human adenylate cyclase 1 (brain) (ADCY1)



  "NM_021116" in other vectors (6)

Reconstitution Protocol

USD 1,461.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "ADCY1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ADCY1
Synonyms AC1; DFNB44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222738 representing NM_021116
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGGCGCCGCGCGGCGGAGGCGGCGGCGGAGGCGGCGCGGGCGAGCCCGGGGGCGCCGAGCGGG
CGGCCGGGACAAGCCGCCGGCGCGGGCTCCGGGCGTGCGACGAGGAGTTCGCTTGCCCAGAGCTGGAGGC
GCTGTTCCGCGGCTACACGCTGCGGCTGGAGCAGGCGGCCACGCTGAAGGCGCTGGCCGTTCTCAGCCTG
CTGGCGGGCGCGCTGGCGCTGGCCGAGCTGCTGGGCGCGCCGGGGCCCGCGCCCGGCCTGGCCAAGGGCT
CACACCCGGTGCACTGCGTCCTCTTCCTGGCGCTGCTCGTGGTAACCAACGTCCGGTCCCTGCAGGTGCC
CCAGCTGCAGCAGGTCGGCCAGCTGGCGCTGCTCTTCAGCCTCACCTTCGCGCTGCTCTGCTGTCCTTTC
GCGCTGGGCGGCCCCGCCCGGGGTTCCGCCGGGGCCGCTGGGGGGCCAGCGACCGCCGAACAAGGGGTTT
GGCAGCTCCTTTTGGTCACCTTCGTGTCCTATGCCTTGCTGCCCGTGCGCAGCCTGCTGGCCATAGGCTT
TGGGCTCGTGGTGGCTGCGTCGCACTTGCTGGTCACAGCCACCTTGGTCCCCGCCAAGCGCCCACGTCTC
TGGAGGACGCTCGGTGCCAATGCCTTGCTCTTCGTCGGTGTGAACATGTATGGGGTCTTTGTGCGGATTC
TGACTGAGCGTTCACAGAGGAAGGCGTTCCTGCAGGCCCGGAGCTGCATTGAGGACCGACTGAGGCTGGA
GGATGAGAACGAGAAGCAGGAGCGGCTCCTCATGAGCCTCCTGCCCCGGAACGTTGCCATGGAGATGAAG
GAGGACTTCCTGAAGCCCCCTGAGAGGATTTTCCACAAGATTTACATCCAGAGGCACGACAATGTGAGCA
TCCTGTTTGCTGACATCGTGGGTTTCACGGGCTTGGCATCCCAGTGCACAGCCCAGGAGCTGGTGAAACT
CCTCAATGAGCTCTTCGGCAAGTTCGATGAATTAGCCACGGAGAACCACTGTCGCCGCATCAAGATTCTC
GGGGACTGCTACTACTGCGTGTCGGGCCTCACCCAGCCCAAGACTGACCATGCCCACTGCTGTGTGGAGA
TGGGACTCGACATGATTGATACCATCACATCTGTGGCTGAAGCCACCGAGGTGGATCTGAACATGCGTGT
GGGTCTGCACACGGGCAGGGTCCTCTGTGGTGTCCTGGGCTTGCGCAAGTGGCAGTACGACGTGTGGTCC
AATGATGTGACCTTGGCCAATGTCATGGAAGCCGCTGGCCTGCCAGGGAAGGTTCATATCACAAAGACGA
CCCTAGCGTGCTTGAATGGGGACTACGAGGTAGAACCGGGTTACGGACATGAGAGGAACAGTTTCTTGAA
AACTCATAACATCGAAACCTTTTTTATTGTGCCATCCCATCGCCGAAAGATATTTCCAGGCCTGATTCTC
TCAGATATAAAACCGGCCAAAAGGATGAAGTTCAAGACTGTCTGCTACCTGCTGGTGCAGCTCATGCACT
GCCGGAAAATGTTCAAGGCCGAGATCCCCTTCTCCAATGTCATGACCTGCGAGGACGATGACAAGCGGAG
GGCATTAAGAACAGCCTCGGAAAAACTCAGAAACCGCTCATCTTTTTCTACCAACGTTGTCTACACCACC
CCGGGCACTCGCGTCAACAGGTACATCAGCCGCCTCTTAGAAGCCCGCCAGACAGAGCTGGAGATGGCAG
ACCTGAACTTCTTTACCCTGAAGTACAAACATGTCGAACGGGAGCAAAAGTACCACCAGCTTCAGGACGA
GTATTTCACCAGCGCCGTTGTCCTCACCCTCATCCTGGCTGCCTTATTTGGCCTTGTCTACCTTCTAATA
TTCCCACAGAGTGTGGTCGTCCTGCTCCTGCTAGTATTCTGCATCTGCTTCCTGGTGGCCTGTGTCCTGT
ACCTGCACATCACCCGGGTCCAGTGTTTTCCAGGGTGCCTGACGATTCAGATTCGCACTGTCCTGTGTAT
TTTCATAGTGGTCTTAATCTACTCAGTAGCCCAAGGTTGTGTGGTGGGCTGCCTGCCTTGGGCCTGGAGC
TCCAAGCCCAACAGTTCCCTGGTGGTCCTTTCGTCTGGGGGCCAGCGCACAGCCCTGCCCACCCTGCCCT
GCGAGTCTACACACCATGCCCTGCTCTGCTGCCTGGTGGGCACCCTCCCGCTAGCCATATTTTTCCGGGT
GTCCTCCTTGCCAAAAATGATCCTGCTCTCCGGGCTCACCACGTCCTACATCCTCGTTCTGGAGCTCAGC
GGATACACCAGGACTGGGGGTGGTGCCGTCTCCGGGCGCAGCTACGAGCCGATTGTGGCCATCCTGCTCT
TCTCCTGTGCGCTGGCCCTGCATGCCAGGCAGGTGGACATCAGGCTGAGGCTGGACTACCTCTGGGCCGC
ACAGGCAGAGGAGGAGCGAGAGGACATGGAGAAGGTGAAGCTGGACAACAGGCGCATCCTCTTCAACCTC
CTGCCGGCCCACGTCGCCCAGCACTTCCTCATGTCCAACCCTCGGAACATGGACCTCTACTACCAGTCCT
ACTCCCAGGTGGGCGTCATGTTTGCCTCCATCCCCAACTTCAATGACTTCTACATCGAGCTGGACGGCAA
CAACATGGGGGTGGAGTGTCTGCGGCTTCTCAACGAGATCATCGCCGACTTTGACGAGCTCATGGAAAAA
GACTTTTACAAGGACATAGAGAAGATCAAGACCATCGGGAGCACCTACATGGCCGCTGTGGGGCTAGCGC
CCACCTCGGGGACCAAGGCTAAGAAGTCCATCTCCTCCCACCTGAGCACGCTGGCGGACTTTGCCATTGA
GATGTTTGACGTTCTGGATGAAATCAACTACCAGTCTTACAACGACTTTGTCCTCCGAGTTGGCATCAAT
GTTGGCCCTGTGGTGGCTGGAGTGATTGGCGCTCGCAGGCCCCAGTACGACATCTGGGGAAACACAGTCA
ACGTGGCCAGTCGGATGGATAGCACAGGGGTCCAGGGCAGAATCCAGGTGACTGAGGAAGTCCACCGGCT
GCTGAGAAGGTGCCCCTACCACTTTGTGTGCCGAGGCAAAGTCAGTGTCAAGGGCAAAGGCGAGATGTTG
ACATACTTTCTAGAAGGCAGGACTGATGGAAACGGCTCCCAAATCAGGTCCCTGGGCTTGGATCGGAAAA
TGTGTCCATTTGGGAGAGCTGGCCTTCAGGGCAGACGTCCCCCCGTGTGCCCCATGCCTGGCGTCTCAGT
CAGGGCTGGGCTCCCTCCACACTCCCCAGGCCAGTACCTGCCCTCTGCAGCAGCTGGGAAGGAGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222738 representing NM_021116
Red=Cloning site Green=Tags(s)

MAGAPRGGGGGGGGAGEPGGAERAAGTSRRRGLRACDEEFACPELEALFRGYTLRLEQAATLKALAVLSL
LAGALALAELLGAPGPAPGLAKGSHPVHCVLFLALLVVTNVRSLQVPQLQQVGQLALLFSLTFALLCCPF
ALGGPARGSAGAAGGPATAEQGVWQLLLVTFVSYALLPVRSLLAIGFGLVVAASHLLVTATLVPAKRPRL
WRTLGANALLFVGVNMYGVFVRILTERSQRKAFLQARSCIEDRLRLEDENEKQERLLMSLLPRNVAMEMK
EDFLKPPERIFHKIYIQRHDNVSILFADIVGFTGLASQCTAQELVKLLNELFGKFDELATENHCRRIKIL
GDCYYCVSGLTQPKTDHAHCCVEMGLDMIDTITSVAEATEVDLNMRVGLHTGRVLCGVLGLRKWQYDVWS
NDVTLANVMEAAGLPGKVHITKTTLACLNGDYEVEPGYGHERNSFLKTHNIETFFIVPSHRRKIFPGLIL
SDIKPAKRMKFKTVCYLLVQLMHCRKMFKAEIPFSNVMTCEDDDKRRALRTASEKLRNRSSFSTNVVYTT
PGTRVNRYISRLLEARQTELEMADLNFFTLKYKHVEREQKYHQLQDEYFTSAVVLTLILAALFGLVYLLI
FPQSVVVLLLLVFCICFLVACVLYLHITRVQCFPGCLTIQIRTVLCIFIVVLIYSVAQGCVVGCLPWAWS
SKPNSSLVVLSSGGQRTALPTLPCESTHHALLCCLVGTLPLAIFFRVSSLPKMILLSGLTTSYILVLELS
GYTRTGGGAVSGRSYEPIVAILLFSCALALHARQVDIRLRLDYLWAAQAEEEREDMEKVKLDNRRILFNL
LPAHVAQHFLMSNPRNMDLYYQSYSQVGVMFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDELMEK
DFYKDIEKIKTIGSTYMAAVGLAPTSGTKAKKSISSHLSTLADFAIEMFDVLDEINYQSYNDFVLRVGIN
VGPVVAGVIGARRPQYDIWGNTVNVASRMDSTGVQGRIQVTEEVHRLLRRCPYHFVCRGKVSVKGKGEML
TYFLEGRTDGNGSQIRSLGLDRKMCPFGRAGLQGRRPPVCPMPGVSVRAGLPPHSPGQYLPSAAAGKEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021116
ORF Size 3357 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021116.4
RefSeq Size 12499 bp
RefSeq ORF 3360 bp
Locus ID 107
UniProt ID Q08828
Cytogenetics 7p12.3
Protein Families Druggable Genome, Transmembrane
Protein Pathways Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
MW 123.3 kDa
Gene Summary This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

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