Adenylate cyclase 1 (ADCY1) (NM_021116) Human Tagged ORF Clone

CAT#: RC222738

ADCY1 (Myc-DDK-tagged)-Human adenylate cyclase 1 (brain) (ADCY1)

Product Images

Circular map for RC222738

• 

  Western blot validation of overexpression lysate (Cat# LY412077) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222738 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adenylate cyclase 1
• Synonyms: AC1; DFNB44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222738 representing NM_021116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGCGCCGCGCGGCGGAGGCGGCGGCGGAGGCGGCGCGGGCGAGCCCGGGGGCGCCGAGCGGG
  CGGCCGGGACAAGCCGCCGGCGCGGGCTCCGGGCGTGCGACGAGGAGTTCGCTTGCCCAGAGCTGGAGGC
  GCTGTTCCGCGGCTACACGCTGCGGCTGGAGCAGGCGGCCACGCTGAAGGCGCTGGCCGTTCTCAGCCTG
  CTGGCGGGCGCGCTGGCGCTGGCCGAGCTGCTGGGCGCGCCGGGGCCCGCGCCCGGCCTGGCCAAGGGCT
  CACACCCGGTGCACTGCGTCCTCTTCCTGGCGCTGCTCGTGGTAACCAACGTCCGGTCCCTGCAGGTGCC
  CCAGCTGCAGCAGGTCGGCCAGCTGGCGCTGCTCTTCAGCCTCACCTTCGCGCTGCTCTGCTGTCCTTTC
  GCGCTGGGCGGCCCCGCCCGGGGTTCCGCCGGGGCCGCTGGGGGGCCAGCGACCGCCGAACAAGGGGTTT
  GGCAGCTCCTTTTGGTCACCTTCGTGTCCTATGCCTTGCTGCCCGTGCGCAGCCTGCTGGCCATAGGCTT
  TGGGCTCGTGGTGGCTGCGTCGCACTTGCTGGTCACAGCCACCTTGGTCCCCGCCAAGCGCCCACGTCTC
  TGGAGGACGCTCGGTGCCAATGCCTTGCTCTTCGTCGGTGTGAACATGTATGGGGTCTTTGTGCGGATTC
  TGACTGAGCGTTCACAGAGGAAGGCGTTCCTGCAGGCCCGGAGCTGCATTGAGGACCGACTGAGGCTGGA
  GGATGAGAACGAGAAGCAGGAGCGGCTCCTCATGAGCCTCCTGCCCCGGAACGTTGCCATGGAGATGAAG
  GAGGACTTCCTGAAGCCCCCTGAGAGGATTTTCCACAAGATTTACATCCAGAGGCACGACAATGTGAGCA
  TCCTGTTTGCTGACATCGTGGGTTTCACGGGCTTGGCATCCCAGTGCACAGCCCAGGAGCTGGTGAAACT
  CCTCAATGAGCTCTTCGGCAAGTTCGATGAATTAGCCACGGAGAACCACTGTCGCCGCATCAAGATTCTC
  GGGGACTGCTACTACTGCGTGTCGGGCCTCACCCAGCCCAAGACTGACCATGCCCACTGCTGTGTGGAGA
  TGGGACTCGACATGATTGATACCATCACATCTGTGGCTGAAGCCACCGAGGTGGATCTGAACATGCGTGT
  GGGTCTGCACACGGGCAGGGTCCTCTGTGGTGTCCTGGGCTTGCGCAAGTGGCAGTACGACGTGTGGTCC
  AATGATGTGACCTTGGCCAATGTCATGGAAGCCGCTGGCCTGCCAGGGAAGGTTCATATCACAAAGACGA
  CCCTAGCGTGCTTGAATGGGGACTACGAGGTAGAACCGGGTTACGGACATGAGAGGAACAGTTTCTTGAA
  AACTCATAACATCGAAACCTTTTTTATTGTGCCATCCCATCGCCGAAAGATATTTCCAGGCCTGATTCTC
  TCAGATATAAAACCGGCCAAAAGGATGAAGTTCAAGACTGTCTGCTACCTGCTGGTGCAGCTCATGCACT
  GCCGGAAAATGTTCAAGGCCGAGATCCCCTTCTCCAATGTCATGACCTGCGAGGACGATGACAAGCGGAG
  GGCATTAAGAACAGCCTCGGAAAAACTCAGAAACCGCTCATCTTTTTCTACCAACGTTGTCTACACCACC
  CCGGGCACTCGCGTCAACAGGTACATCAGCCGCCTCTTAGAAGCCCGCCAGACAGAGCTGGAGATGGCAG
  ACCTGAACTTCTTTACCCTGAAGTACAAACATGTCGAACGGGAGCAAAAGTACCACCAGCTTCAGGACGA
  GTATTTCACCAGCGCCGTTGTCCTCACCCTCATCCTGGCTGCCTTATTTGGCCTTGTCTACCTTCTAATA
  TTCCCACAGAGTGTGGTCGTCCTGCTCCTGCTAGTATTCTGCATCTGCTTCCTGGTGGCCTGTGTCCTGT
  ACCTGCACATCACCCGGGTCCAGTGTTTTCCAGGGTGCCTGACGATTCAGATTCGCACTGTCCTGTGTAT
  TTTCATAGTGGTCTTAATCTACTCAGTAGCCCAAGGTTGTGTGGTGGGCTGCCTGCCTTGGGCCTGGAGC
  TCCAAGCCCAACAGTTCCCTGGTGGTCCTTTCGTCTGGGGGCCAGCGCACAGCCCTGCCCACCCTGCCCT
  GCGAGTCTACACACCATGCCCTGCTCTGCTGCCTGGTGGGCACCCTCCCGCTAGCCATATTTTTCCGGGT
  GTCCTCCTTGCCAAAAATGATCCTGCTCTCCGGGCTCACCACGTCCTACATCCTCGTTCTGGAGCTCAGC
  GGATACACCAGGACTGGGGGTGGTGCCGTCTCCGGGCGCAGCTACGAGCCGATTGTGGCCATCCTGCTCT
  TCTCCTGTGCGCTGGCCCTGCATGCCAGGCAGGTGGACATCAGGCTGAGGCTGGACTACCTCTGGGCCGC
  ACAGGCAGAGGAGGAGCGAGAGGACATGGAGAAGGTGAAGCTGGACAACAGGCGCATCCTCTTCAACCTC
  CTGCCGGCCCACGTCGCCCAGCACTTCCTCATGTCCAACCCTCGGAACATGGACCTCTACTACCAGTCCT
  ACTCCCAGGTGGGCGTCATGTTTGCCTCCATCCCCAACTTCAATGACTTCTACATCGAGCTGGACGGCAA
  CAACATGGGGGTGGAGTGTCTGCGGCTTCTCAACGAGATCATCGCCGACTTTGACGAGCTCATGGAAAAA
  GACTTTTACAAGGACATAGAGAAGATCAAGACCATCGGGAGCACCTACATGGCCGCTGTGGGGCTAGCGC
  CCACCTCGGGGACCAAGGCTAAGAAGTCCATCTCCTCCCACCTGAGCACGCTGGCGGACTTTGCCATTGA
  GATGTTTGACGTTCTGGATGAAATCAACTACCAGTCTTACAACGACTTTGTCCTCCGAGTTGGCATCAAT
  GTTGGCCCTGTGGTGGCTGGAGTGATTGGCGCTCGCAGGCCCCAGTACGACATCTGGGGAAACACAGTCA
  ACGTGGCCAGTCGGATGGATAGCACAGGGGTCCAGGGCAGAATCCAGGTGACTGAGGAAGTCCACCGGCT
  GCTGAGAAGGTGCCCCTACCACTTTGTGTGCCGAGGCAAAGTCAGTGTCAAGGGCAAAGGCGAGATGTTG
  ACATACTTTCTAGAAGGCAGGACTGATGGAAACGGCTCCCAAATCAGGTCCCTGGGCTTGGATCGGAAAA
  TGTGTCCATTTGGGAGAGCTGGCCTTCAGGGCAGACGTCCCCCCGTGTGCCCCATGCCTGGCGTCTCAGT
  CAGGGCTGGGCTCCCTCCACACTCCCCAGGCCAGTACCTGCCCTCTGCAGCAGCTGGGAAGGAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222738 representing NM_021116
  Red=Cloning site Green=Tags(s)
  
  MAGAPRGGGGGGGGAGEPGGAERAAGTSRRRGLRACDEEFACPELEALFRGYTLRLEQAATLKALAVLSL
  LAGALALAELLGAPGPAPGLAKGSHPVHCVLFLALLVVTNVRSLQVPQLQQVGQLALLFSLTFALLCCPF
  ALGGPARGSAGAAGGPATAEQGVWQLLLVTFVSYALLPVRSLLAIGFGLVVAASHLLVTATLVPAKRPRL
  WRTLGANALLFVGVNMYGVFVRILTERSQRKAFLQARSCIEDRLRLEDENEKQERLLMSLLPRNVAMEMK
  EDFLKPPERIFHKIYIQRHDNVSILFADIVGFTGLASQCTAQELVKLLNELFGKFDELATENHCRRIKIL
  GDCYYCVSGLTQPKTDHAHCCVEMGLDMIDTITSVAEATEVDLNMRVGLHTGRVLCGVLGLRKWQYDVWS
  NDVTLANVMEAAGLPGKVHITKTTLACLNGDYEVEPGYGHERNSFLKTHNIETFFIVPSHRRKIFPGLIL
  SDIKPAKRMKFKTVCYLLVQLMHCRKMFKAEIPFSNVMTCEDDDKRRALRTASEKLRNRSSFSTNVVYTT
  PGTRVNRYISRLLEARQTELEMADLNFFTLKYKHVEREQKYHQLQDEYFTSAVVLTLILAALFGLVYLLI
  FPQSVVVLLLLVFCICFLVACVLYLHITRVQCFPGCLTIQIRTVLCIFIVVLIYSVAQGCVVGCLPWAWS
  SKPNSSLVVLSSGGQRTALPTLPCESTHHALLCCLVGTLPLAIFFRVSSLPKMILLSGLTTSYILVLELS
  GYTRTGGGAVSGRSYEPIVAILLFSCALALHARQVDIRLRLDYLWAAQAEEEREDMEKVKLDNRRILFNL
  LPAHVAQHFLMSNPRNMDLYYQSYSQVGVMFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDELMEK
  DFYKDIEKIKTIGSTYMAAVGLAPTSGTKAKKSISSHLSTLADFAIEMFDVLDEINYQSYNDFVLRVGIN
  VGPVVAGVIGARRPQYDIWGNTVNVASRMDSTGVQGRIQVTEEVHRLLRRCPYHFVCRGKVSVKGKGEML
  TYFLEGRTDGNGSQIRSLGLDRKMCPFGRAGLQGRRPPVCPMPGVSVRAGLPPHSPGQYLPSAAAGKEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021116
• ORF Size: 3357 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021116.4
• RefSeq Size: 12499 bp
• RefSeq ORF: 3360 bp
• Locus ID: 107
• UniProt ID: Q08828
• Cytogenetics: 7p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
• MW: 123.3 kDa
• Gene Summary: This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]