TMEM16B (ANO2) (NM_020373) Human Tagged ORF Clone

CAT#: RC222601

ANO2 (Myc-DDK-tagged)-Human anoctamin 2 (ANO2)

Product images

Western blot validation of overexpression lysate (Cat# LY412515) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222601 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: TMEM16B (ANO2) (NM_020373) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANO2
• Synonyms: C12orf3; TMEM16B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222601 representing NM_020373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCAGGGTCCAGAGGGG
  GCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGGGCCCCAGGTCTGCAGGGCGG
  TTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGCACCCGCAGCAGCTCTGTCATCAACAACTAT
  CTGGATGCCAATGAGCCTGTGTCCTTGGAGGCCCGTCTTAGCCGCATGCACTTCCATGACAGTCAGAGGA
  AGGTCGACTATGTACTTGCCTACCACTACCGGAAACGCGGGGTGCACCTGGCCCAAGGCTTCCCTGGCCA
  CTCGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCAGGTGACATTGAG
  CTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAATTTGAGCACAATCTGATGG
  AGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCCATCTTTGTCCGGATACACGC
  CCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAGATCAAAGTTCCTACCAAGAAAGAGATGTAC
  GAGATCAAAGCAGGAGGCAGCATTGCAAAGAAGTTCAGCGCGGCTCTGCAGAAGCTGAGCTCGCACCTGC
  AGCCCCGAGTTCCAGAACACAGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTCCAGGGAGAAGAT
  GTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGTAGCCGCATTGTGCACGAG
  ATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTCTGATCGCAAACAATA
  TCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTCCAGAGGACGATATGAATGACAGGAA
  GCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTATAAGTTCCAACCTATTGACCTCATCAGAAAG
  TATTTTGGAGAAAAAATTGGACTGTATTTTGCCTGGCTGGGATTATATACATCATTCCTCATCCCATCTT
  CTGTAATTGGAGTGATTGTGTTTCTTTATGGATGTGCAACAATTGAAGAAGATATTCCCAGCAGAGAGAT
  GTGTGACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGATTACTGGAACCTCAGC
  TCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACCGTCTTCTTCTCTATCTTCA
  TGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTACAGATGCGACTGGGCTACTTTTGGGA
  CCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAGGAACATTCCAGGCCTGAGTATGAAACCAAAGTT
  CGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCTGTCCAGAAATTGGAAACAAACACGACGGAGTGTG
  GCGATGAGGATGATGAAGATAAACTGACCTGGAAGGATCGTTTCCCAGGTTACCTGATGAACTTTGCCTC
  CATCTTATTCATGATTGCCCTGACATTCTCAATCGTCTTTGGGGTGATAGTGTATCGAATAACAACTGCA
  GCCGCTCTGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTGACAGTGACAGCAACAGCAGTCATCA
  TCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCTGTGGCCAAGTGGCTCACCAAAATTGA
  GGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATCCTCAAAGCTTTCTTGCTCAAGTTTGTCAAT
  GCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAAGGGAGGTTTGTGGGCAGGCCTGGAAGCTACGTCT
  ATGTATTCGATGGTTACCGCATGGAAGAGTGTGCTCCAGGGGGCTGTCTCATGGAGCTCTGCATTCAGCT
  CAGCATCATCATGTTGGGGAAGCAGTTGATCCAGAACAACATCTTTGAGATTGGAGTCCCGAAGCTAAAG
  AAACTATTTCGAAAGCTGAAAGATGAGACCGAAGCTGGAGAAACTGACTCTGCCCATTCGAAACATCCAG
  AGCAGTGGGACCTAGACTACAGCTTGGAACCATACACAGGACTGACTCCGGAGTACATGGAAATGATCAT
  CCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTTCCCCTGGCACCTGTGTTTGCCCTCCTCAACAAC
  GTCATTGAAGTGCGGCTCGATGCAAAGAAGTTTGTTACAGAGCTGAGACGGCCGGATGCTGTAAGAACCA
  AAGATATCGGAATCTGGTTTGACATTCTCTCTGGAATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGT
  CATTGCGATCACCTCCGACTTTATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCACAATGGGACTCTG
  CACGGCTTTGTCAACCACACCCTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGACGCAGCCAGAAA
  ACTCACAGTTTGACCAGGAGGTTCAGTTCTGCAGGTTTAAGGATTACCGAGAGCCGCCATGGGCCCCGAA
  CCCTTATGAGTTTTCGAAACAGTACTGGTTTATTCTGTCTGCCCGTCTGGCTTTTGTCATAATCTTCCAG
  AACCTCGTGATGTTCCTGAGCGTCCTCGTGGACTGGATGATTCCAGACATCCCCACGGACATCAGCGACC
  AGATCAAGAAAGAGAAGAGCTTATTAGTGGATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGAT
  GGATGAGCCGGCTCTGAGGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCT
  TCAGGCCAAAGCCAGCTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222601 representing NM_020373
  Red=Cloning site Green=Tags(s)
  
  MPEDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSSVINNY
  LDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAGGPGDIE
  LGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLKIKVPTKKEMY
  EIKAGGSIAKKFSAALQKLSSHLQPRVPEHSNNKMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRIVHE
  ILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYKFQPIDLIRK
  YFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLCDKSCDYWNLS
  SACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQEHSRPEYETKV
  REKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRITTA
  AALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVN
  AYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLK
  KLFRKLKDETEAGETDSAHSKHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNN
  VIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDFIPRLVYQYSYSHNGTL
  HGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWFILSARLAFVIIFQ
  NLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEPALRSPGGGDRSRSRAASSAP
  SGQSQLGSMMSSGSQHTNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_020373
• ORF Size: 2997 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020373.1, NM_020373.2, NP_065106.1
• RefSeq Size: 3717 bp
• RefSeq ORF: 2996 bp
• Locus ID: 57101
• Cytogenetics: 12p13.31
• Protein Families: Transmembrane
• MW: 113.3 kDa
• Gene Summary: ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]). [supplied by OMIM, Jan 2011]