kynurenine 3 monooxygenase (KMO) (NM_003679) Human Tagged ORF Clone

CAT#: RC222594

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KMO (Myc-DDK-tagged)-Human kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) (KMO)



  "NM_003679" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 511.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KMO
Synonyms dJ317G22.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222594 representing NM_003679
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCATCTGTCATTCAAAGGAAAAAAGTAGCTGTCATTGGTGGTGGCTTGGTTGGCTCATTACAAG
CATGCTTTCTTGCAAAGAGGAATTTCCAGATTGATGTATATGAAGCTAGGGAAGATACTCGAGTGGCTAC
CTTCACACGTGGAAGAAGCATTAACTTAGCCCTTTCTCATAGAGGACGACAAGCCTTGAAAGCTGTTGGC
CTGGAAGATCAGATTGTATCCCAAGGTATTCCCATGAGAGCAAGAATGATCCACTCTCTTTCAGGAAAAA
AGTCTGCAATTCCCTATGGGACAAAGTCTCAGTATATTCTTTCTGTAAGCAGAGAAAATCTAAACAAGGA
TCTATTGACTGCTGCTGAGAAATACCCCAATGTGAAAATGCACTTTAACCACAGGCTGTTGAAATGTAAT
CCAGAGGAAGGAATGATCACAGTGCTTGGATCTGACAAAGTTCCCAAAGATGTCACTTGTGACCTCATTG
TAGGATGTGATGGAGCCTATTCAACTGTCAGATCTCACCTGATGAAGAAACCTCGCTTTGATTACAGTCA
GCAGTACATTCCTCATGGGTACATGGAGTTGACTATTCCACCTAAGAACGGAGATTATGCCATGGAACCT
AATTATCTGCATATTTGGCCTAGAAATACCTTTATGATGATTGCACTTCCTAACATGAACAAATCATTCA
CATGTACTTTGTTCATGCCCTTTGAAGAGTTTGAAAAACTTCTAACCAGTAATGATGTGGTAGATTTCTT
CCAGAAATACTTTCCAGATGCCATCCCTCTAATTGGAGAGAAACTCCTAGTGCAAGATTTCTTCCTGTTG
CCTGCCCAGCCCATGATATCTGTAAAGTGCTCTTCATTTCACTTTAAATCTCACTGTGTACTGCTGGGAG
ATGCAGCTCATGCTATAGTGCCGTTTTTTGGGCAAGGAATGAATGCGGGCTTTGAAGACTGCTTGGTATT
TGATGAGTTAATGGATAAATTCAGTAACGACCTTAGTTTGTGTCTTCCTGTGTTCTCAAGATTGAGAATC
CCAGATGATCACGCGATTTCAGACCTATCCATGTACAATTACATAGAGATGCGAGCACATGTCAACTCAA
GCTGGTTCATTTTTCAGAAGAACATGGAGAGATTTCTTCATGCGATTATGCCATCGACCTTTATCCCTCT
CTATACAATGGTCACTTTTTCCAGAATAAGATACCATGAGGCTGTGCAGCGTTGGCATTGGCAAAAAAAG
GTGATAAACAAAGGACTCTTTTTCTTGGGATCACTGATAGCCATCAGCAGTACCTACCTACTTATACACT
ACATGTCACCACGATCTTTCCTCTGCTTGAGAAGACCATGGAACTGGATAGCTCACTTCCGGAATACAAC
ATGTTTCCCCGCAAAGGCCGTGGACTCCCTAGAACAAATTTCCAATCTCATTAGCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222594 representing NM_003679
Red=Cloning site Green=Tags(s)

MDSSVIQRKKVAVIGGGLVGSLQACFLAKRNFQIDVYEAREDTRVATFTRGRSINLALSHRGRQALKAVG
LEDQIVSQGIPMRARMIHSLSGKKSAIPYGTKSQYILSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCN
PEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEP
NYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLL
PAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRI
PDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKK
VINKGLFFLGSLIAISSTYLLIHYMSPRSFLCLRRPWNWIAHFRNTTCFPAKAVDSLEQISNLISR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003679
ORF Size 1458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003679.5
RefSeq Size 4992 bp
RefSeq ORF 1461 bp
Locus ID 8564
UniProt ID O15229
Cytogenetics 1q43
Domains Monooxygenase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Tryptophan metabolism
MW 55.6 kDa
Gene Summary This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease. [provided by RefSeq, Oct 2011]

Other Versions

Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

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Rabbit polyclonal KMO Antibody (Center)
    • 400 ul

USD 512.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

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