CCNK (NM_001099402) Human Tagged ORF Clone

CAT#: RC222559

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CCNK (Myc-DDK-tagged)-Human cyclin K (CCNK)



  "NM_001099402" in other vectors (6)

Reconstitution Protocol

USD 810.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "CCNK"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CCNK
Synonyms CPR4; IDDHDF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222559 representing NM_001099402
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGAATAAAGAAAATTCAAGCCCTTCAGTAACTTCAGCAAACCTGGACCACACAAAGCCATGTT
GGTACTGGGATAAGAAAGACTTGGCTCATACACCCTCACAACTTGAAGGACTTGATCCAGCCACCGAGGC
CCGGTACCGCCGAGAGGGCGCTCGGTTCATCTTTGATGTGGGCACACGTTTGGGGCTACACTATGATACC
CTGGCAACTGGAATAATTTATTTTCATCGCTTCTATATGTTTCATTCCTTCAAGCAATTCCCAAGATATG
TGACAGGAGCCTGTTGCCTCTTTCTGGCTGGGAAAGTAGAAGAAACACCAAAAAAATGTAAAGATATCAT
CAAAACAGCTCGTAGTTTATTAAATGATGTACAATTTGGCCAGTTTGGAGATGACCCAAAGGAGGAAGTA
ATGGTTCTGGAGAGAATCTTACTGCAGACCATCAAGTTTGATTTACAGGTAGAACATCCATACCAGTTCC
TACTAAAATATGCAAAGCAACTCAAAGGTGATAAAAACAAAATTCAAAAGTTGGTTCAAATGGCATGGAC
ATTTGTAAATGACAGTCTCTGCACCACCTTGTCACTGCAGTGGGAACCAGAGATCATAGCAGTAGCAGTG
ATGTATCTCGCAGGACGTTTGTGCAAATTTGAAATACAAGAATGGACCTCCAAACCCATGTATAGGAGAT
GGTGGGAGCAGTTTGTTCAAGATGTCCCGGTCGACGTTTTGGAAGACATCTGCCACCAAATCCTGGATCT
TTACTCACAAGGAAAACAACAGATGCCTCATCACACCCCCCATCAGCTGCAACAGCCCCCATCTCTTCAG
CCTACACCACAAGTGCCGCAAGTACAGCAGTCACAGCCGTCTCAAAGCTCCGAACCATCCCAGCCCCAGC
AGAAGGACCCCCAGCAACCAGCCCAGCAGCAGCAGCCAGCCCAACAGCCCAAGAAACCCTCTCCGCAGCC
CAGTTCTCCCCGACAGGTTAAGCGAGCCGTGGTTGTTTCTCCCAAAGAAGAGAACAAAGCAGCAGAACCA
CCACCACCTAAAATCCCCAAAATTGAGACCACTCATCCACCGTTGCCTCCAGCCCACCCACCTCCAGACC
GGAAGCCTCCCCTCGCTGCTGCCTTAGGTGAGGCTGAGCCGCCGGGCCCTGTGGATGCCACTGACCTCCC
CAAAGTCCAGATTCCCCCTCCGGCCCACCCGGCCCCTGTGCACCAGCCACCGCCGCTGCCACACCGGCCC
CCGCCCCCACCCCCCTCCAGCTACATGACCGGGATGTCCACCACCAGCTCCTACATGTCTGGAGAGGGCT
ACCAGAGCCTGCAGTCCATGATGAAGACCGAGGGACCCTCCTACGGTGCCCTGCCCCCCGCCTACGGCCC
ACCTGCACACCTGCCCTACCACCCCCATGTCTACCCGCCCAACCCGCCCCCGCCACCTGTGCCTCCTCCC
CCAGCCTCCTTCCCCCCACCTGCCATCCCACCCCCTACTCCTGGCTACCCCCCACCCCCACCCACCTACA
ACCCCAACTTCCCACCCCCACCCCCACGCCTCCCGCCTACCCACGCAGTCCCCCCTCATCCTCCTCCAGG
GTTGGGCCTGCCGCCAGCCAGCTACCCACCTCCTGCCGTCCCCCCTGGAGGACAGCCTCCTGTGCCCCCG
CCCATTCCCCCACCCGGCATGCCTCCAGTTGGGGGGCTGGGGCGGGCAGCCTGGATGAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222559 representing NM_001099402
Red=Cloning site Green=Tags(s)

MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDT
LATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEV
MVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAV
MYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQ
PTPQVPQVQQSQPSQSSEPSQPQQKDPQQPAQQQQPAQQPKKPSPQPSSPRQVKRAVVVSPKEENKAAEP
PPPKIPKIETTHPPLPPAHPPPDRKPPLAAALGEAEPPGPVDATDLPKVQIPPPAHPAPVHQPPPLPHRP
PPPPPSSYMTGMSTTSSYMSGEGYQSLQSMMKTEGPSYGALPPAYGPPAHLPYHPHVYPPNPPPPPVPPP
PASFPPPAIPPPTPGYPPPPPTYNPNFPPPPPRLPPTHAVPPHPPPGLGLPPASYPPPAVPPGGQPPVPP
PIPPPGMPPVGGLGRAAWMR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001099402
ORF Size 1740 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001099402.2
RefSeq Size 2618 bp
RefSeq ORF 1743 bp
Locus ID 8812
UniProt ID O75909
Cytogenetics 14q32.2
Protein Families Druggable Genome, Transcription Factors
MW 64.1 kDa
Gene Summary The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities. [provided by RefSeq, Jul 2008]

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