ATG9A (NM_024085) Human Tagged ORF Clone

CAT#: RC222513

ATG9A (Myc-DDK-tagged)-Human ATG9 autophagy related 9 homolog A (S. cerevisiae) (ATG9A), transcript variant 2

Product Images

Circular map for RC222513

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  Western blot validation of overexpression lysate (Cat# LY421380) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222568 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ATG9A protein (Cat# TP322513). The protein was produced from HEK293T cells transfected with ATG9A cDNA clone (Cat# RC222513) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG9A
• Synonyms: APG9L1; mATG9; MGD3208
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222513 representing NM_024085
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGTGATTCACCCCCAGGGGAGGAGG
  ACCTGTTGGTGCACGTCGCCGAGGGGAGCAAGTCACCTTGGCACCATATTGAAAACCTTGACCTCTTCTT
  CTCTCGAGTTTATAATCTGCACCAGAAGAATGGCTTCACATGTATGCTCATCGGGGAGATCTTTGAGCTC
  ATGCAGTTCCTCTTTGTGGTTGCCTTCACTACCTTCCTGGTCAGCTGCGTGGACTATGACATCCTATTTG
  CCAACAAGATGGTGAACCACAGTCTTCACCCTACTGAACCCGTCAAGGTCACTCTGCCAGACGCCTTTTT
  GCCTGCTCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCCCTTATCACCATCCTGGTCATTGCTGGT
  GTCTTCTGGATCCACCGGCTTATCAAGTTCATCTATAACATTTGCTGCTACTGGGAGATCCACTCCTTCT
  ACCTGCACGCTCTGCGCATCCCTATGTCTGCCCTTCCGTATTGCACGTGGCAAGAAGTGCAGGCCCGGAT
  CGTGCAGACGCAGAAGGAGCACCAGATCTGCATCCACAAACGTGAGCTGACAGAACTGGACATCTACCAC
  CGCATCCTCCGTTTCCAGAACTACATGGTGGCACTGGTTAACAAATCCCTCCTGCCTCTGCGCTTCCGCC
  TGCCTGGCCTCGGGGAAGCTGTCTTCTTCACCCGTGGTCTCAAGTACAACTTTGAGCTGATCCTCTTCTG
  GGGACCTGGCTCTCTGTTTCTCAATGAATGGAGCCTCAAGGCCGAGTACAAACGTGGGGGGCAACGGCTA
  GAGCTGGCCCAGCGCCTCAGCAACCGCATCCTGTGGATTGGCATCGCTAACTTCCTGCTGTGCCCCCTCA
  TCCTCATATGGCAAATCCTCTATGCCTTCTTCAGCTATGCTGAGGTGCTGAAGCGGGAGCCGGGGGCCCT
  GGGAGCACGCTGCTGGTCACTCTATGGCCGCTGCTACCTCCGCCACTTCAACGAGCTGGAGCACGAGCTG
  CAGTCCCGCCTCAACCGTGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCTCTTTTGA
  CACTGCTGGCCAAGAATGGAGCCTTCTTCGCTGGCTCCATCCTGGCTGTGCTTATTGCCCTCACCATTTA
  TGACGAAGATGTGTTGGCTGTGGAACATGTGCTGACCACCGTCACACTCCTGGGGGTCACCGTGACCGTG
  TGCAGGTCCTTTATCCCGGACCAGCACATGGTGTTCTGCCCTGAGCAGCTGCTCCGCGTGATCCTCGCTC
  ACATCCACTACATGCCTGACCACTGGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCA
  GCTCTTCCAGTACAAGGCAGTGTTCATTTTGGAAGAGTTGCTGAGCCCCATTGTCACACCCCTCATCCTC
  ATCTTCTGCCTGCGCCCACGGGCCCTGGAGATTATAGACTTCTTCCGAAACTTCACCGTGGAGGTCGTTG
  GTGTGGGAGATACCTGCTCCTTTGCTCAGATGGATGTTCGCCAGCATGGTCATCCCCAGTGGCTATCTGC
  TGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCTGAGGATGGAAAGACAGAGTTGTCACTCATGCACTTT
  GCCATCACCAACCCTGGCTGGCAGCCACCACGTGAGAGCACAGCCTTCCTAGGCTTCCTCAAGGAGCAGG
  TTCAGCGGGATGGAGCAGCTGCTAGCCTCGCCCAAGGGGGTCTGCTCCCTGAAAATGCCCTCTTTACGTC
  TATCCAGTCCTTACAATCTGAGTCTGAGCCCCTGAGCCTTATCGCAAATGTGGTAGCTGGCTCATCCTGC
  CGGGGCCCTCCACTGCCCAGAGACCTGCAGGGCTCCAGGCACAGGGCTGAAGTCGCCTCTGCCCTGCGCT
  CCTTCTCCCCGCTGCAACCCGGGCAGGCGCCCACAGGCCGGGCTCACAGCACCATGACAGGCTCTGGGGT
  GGATGCCAGGACAGCCAGCTCCGGGAGCAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCAGAA
  TATGCATCCACAGAGATGAGCCTGCATGCCCTCTATATGCACCAGCTCCACAAGCAGCAGGCCCAGGCTG
  AACCTGAGCGGCATGTATGGCACCGCCGGGAGAGTGATGAGAGTGGAGAAAGCGCCCCTGATGAAGGGGG
  AGAGGGCGCCCGGGCCCCCCAGTCTATCCCTCGCTCTGCTAGCTATCCCTGTGCAGCACCCCGGCCTGGA
  GCTCCTGAGACCACCGCCCTGCATGGGGGCTTCCAGAGGCGCTACGGTGGCATCACAGATCCTGGCACAG
  TGCCCAGGGTTCCCTCTCATTTCTCTCGGCTGCCTCTTGGAGGGTGGGCAGAAGATGGGCAGTCGGCATC
  AAGGCACCCTGAGCCCGTGCCCGAAGAGGGCTCGGAGGATGAGCTACCCCCTCAGGTGCACAAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222513 representing NM_024085
  Red=Cloning site Green=Tags(s)
  
  MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEIFEL
  MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG
  VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH
  RILRFQNYMVALVNKSLLPLRFRLPGLGEAVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL
  ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL
  QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV
  CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLIL
  IFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSAGQTEASVYQQAEDGKTELSLMHF
  AITNPGWQPPRESTAFLGFLKEQVQRDGAAASLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSC
  RGPPLPRDLQGSRHRAEVASALRSFSPLQPGQAPTGRAHSTMTGSGVDARTASSGSSVWEGQLQSLVLSE
  YASTEMSLHALYMHQLHKQQAQAEPERHVWHRRESDESGESAPDEGGEGARAPQSIPRSASYPCAAPRPG
  APETTALHGGFQRRYGGITDPGTVPRVPSHFSRLPLGGWAEDGQSASRHPEPVPEEGSEDELPPQVHKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024085
• ORF Size: 2517 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024085.5
• RefSeq Size: 3816 bp
• RefSeq ORF: 2520 bp
• Locus ID: 79065
• UniProt ID: Q7Z3C6
• Cytogenetics: 2q35
• Domains: APG9
• Protein Families: Transmembrane
• MW: 94.3 kDa
• Gene Summary: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.[UniProtKB/Swiss-Prot Function]