Proprotein Convertase 2 (PCSK2) (NM_002594) Human Tagged ORF Clone

CAT#: RC222498

PCSK2 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419211) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222498 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PCSK2 protein (Cat# TP322498). The protein was produced from HEK293T cells transfected with PCSK2 cDNA clone (Cat# RC222498) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCSK2
• Synonyms: NEC-2; NEC 2; NEC2; PC2; SPC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222498 representing NM_002594
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGTGGTTGTGTCTCCCAGTGGAAGGCGGCCGCCGGGTTCCTCTTCTGTGTCATGGTTTTTGCAT
  CTGCTGAGCGACCGGTCTTCACGAATCATTTTCTTGTGGAGTTGCATAAAGGGGGAGAGGACAAAGCTCG
  CCAAGTTGCAGCAGAACACGGCTTTGGAGTCCGAAAGCTTCCCTTTGCTGAAGGTCTGTACCACTTTTAT
  CACAATGGCCTTGCAAAGGCCAAGAGAAGACGCAGCCTACACCACAAGCAGCAGCTGGAGAGAGACCCCA
  GGGTAAAGATGGCTTTGCAGCAGGAAGGATTTGACCGAAAAAAGCGAGGTTACAGAGACATCAATGAGAT
  CGACATCAACATGAACGATCCTCTTTTTACAAAGCAGTGGTATCTGATCAATACTGGGCAAGCTGATGGC
  ACTCCTGGCCTTGATTTGAATGTGGCTGAAGCCTGGGAGCTGGGATACACAGGGAAAGGTGTTACCATTG
  GAATTATGGATGATGGGATTGACTATCTCCACCCGGACCTGGCCTCCAACTATAATGCCGAAGCAAGTTA
  CGACTTCAGCAGCAACGACCCCTATCCTTACCCTCGGTACACAGATGACTGGTTTAACAGCCACGGGACC
  CGATGTGCAGGAGAAGTTTCTGCTGCCGCCAACAACAATATCTGTGGAGTTGGAGTAGCATACAACTCCA
  AGGTTGCAGGCATCCGGATGCTGGACCAGCCATTCATGACAGACATCATCGAGGCCTCCTCCATCAGTCA
  TATGCCACAGCTGATTGACATCTACAGCGCCAGCTGGGGCCCCACAGACAACGGCAAGACAGTGGATGGG
  CCCCGGGAGCTCACGCTGCAGGCCATGGCCGATGGCGTGAACAAGGGCCGCGGCGGCAAAGGCAGCATCT
  ACGTGTGGGCCTCCGGGGACGGCGGCAGCTATGACGACTGCAACTGCGACGGCTACGCCTCCAGCATGTG
  GACCATCTCCATCAACTCAGCCATCAACGACGGCAGGACTGCCCTGTACGACGAGAGCTGCTCTTCCACC
  TTGGCTTCCACCTTCAGCAACGGGAGGAAAAGGAACCCCGAGGCCGGTGTGGCAACCACAGATTTGTACG
  GCAACTGCACTCTGAGGCATTCTGGGACATCTGCAGCTGCCCCCGAGGCAGCTGGTGTGTTTGCACTGGC
  TCTGGAGGCTAACCTGGGTCTGACCTGGCGGGACATGCAGCATCTGACTGTGCTCACCTCCAAACGGAAC
  CAGCTTCACGACGAGGTCCATCAGTGGCGGCGCAATGGGGTCGGCCTGGAATTTAATCACCTCTTTGGCT
  ACGGGGTCCTTGATGCAGGTGCCATGGTGAAAATGGCTAAAGACTGGAAAACCGTGCCTGAGAGATTCCA
  CTGTGTGGGAGGCTCCGTGCAGGACCCTGAGAAAATACCATCCACTGGCAAGTTGGTGCTGACACTCACA
  ACCGACGCCTGTGAGGGGAAGGAAAATTTTGTCCGCTACCTGGAGCATGTCCAGGCTGTCATCACGGTCA
  ACGCAACCAGAAGAGGAGACCTGAACATCAACATGACTTCCCCTATGGGCACCAAGTCCATTTTGCTGAG
  CCGGCGTCCAAGGGATGACGACTCCAAGGTGGGCTTTGACAAGTGGCCTTTCATGACCACTCACACGTGG
  GGGGAAGACGCCCGAGGCACCTGGACCCTGGAGCTGGGATTTGTCGGCAGCGCCCCGCAGAAGGGGGTGC
  TGAAGGAGTGGACCCTGATGCTGCATGGCACTCAGAGTGCCCCGTACATCGACCAGGTGGTGCGGGATTA
  CCAGTCCAAGTTGGCCATGTCCAAGAAAGAGGAGCTGGAGGAAGAGCTGGACGAAGCCGTGGAGAGAAGC
  CTGAAAAGCATCCTTAACAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222498 representing NM_002594
  Red=Cloning site Green=Tags(s)
  
  MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFY
  HNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADG
  TPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGT
  RCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDG
  PRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSST
  LASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRN
  QLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLT
  TDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTW
  GEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERS
  LKSILNKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002594
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002594.5
• RefSeq Size: 4745 bp
• RefSeq ORF: 1917 bp
• Locus ID: 5126
• UniProt ID: P16519
• Cytogenetics: 20p12.1
• Domains: Peptidase_S8, P
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 70.57 kDa
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014]