DGKA (NM_001345) Human Tagged ORF Clone
Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, Myc-DDK-tagged
|Product Name||DGKA (NM_001345) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||DAGK; DAGK1; DGK-alpha|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||2205 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|Domains||DAGKa, DAGKc, EFh, DAG_PE-bind|
|Protein Families||Druggable Genome|
|Protein Pathways||Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system|
|Gene Summary||The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]|
|cDNA Clone Resources|
|SC119317||DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3||
|RC222395||DGKA (Myc-DDK-tagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3||
|RG222395||DGKA (GFP-tagged) - Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3||
|RC222395L1||Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, Myc-DDK-tagged||
|RC222395L2||Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, mGFP tagged||
|RC222395L4||Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, mGFP tagged||