PION (GSAP) (NM_017439) Human Tagged ORF Clone

CAT#: RC222360

PION (Myc-DDK-tagged)-Human pigeon homolog (Drosophila) (PION)

Product Images

Circular map for RC222360

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PION
• Synonyms: PION
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222360 representing NM_017439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTTCGCCTGGTCGCCGACTTCGACCTCGGGAAGGACGTGCTCCCGTGGTTGCGGGCGCAGCGGG
  CAGTGTCGGAGGCCAGCGGGGCCGGAAGCGGCGGCGCAGATGTTTTAGAAAACGATTATGAGAGCTTACA
  TGTATTAAATGTTGAAAGAAATGGAAATATTATTTATACCTATAAGGATGATAAGGGAAATGTCGTCTTT
  GGATTATATGATTGTCAAACCAGACAAAATGAGCTTCTATATACCTTTGAGAAAGACTTGCAAGTTTTCA
  GTTGCTCTGTCAACAGTGAAAGGACTTTGCTTGCTGCAAGTTTAGTTCAGTCTACTAAAGAAGGAAAAAG
  GAACGAACTTCAACCAGGATCAAAGTGCTTGACTTTGTTGGTTGAAATCCACCCTGTTAACAATGTGAAG
  GTTCTAAAGGCTGTGGATAGCTATATTTGGGTTCAGTTTCTCTACCCACATATTGAAAGTCATCCTCTTC
  CAGAGAACCATCTGTTACTGATTTCAGAAGAGAAATATATTGAACAATTTCGTATCCATGTCGCCCAAGA
  AGATGGAAATAGAGTGGTGATTAAAAATTCTGGCCATCTCCCAAGAGACAGAATAGCTGAGGATTTCGTT
  TGGGCTCAGTGGGATATGTCAGAACAGAGATTATATTACATTGACCTGAAGAAATCAAGGAGTATCTTAA
  AATGTATCCAGTTTTATGCTGATGAGAGCTATAACTTAATGTTTGAAGTACCCTTGGACATATCATTAAG
  CAACTCAGGATTTAAACTTGTCAACTTTGGATGTGATTATCATCAATACCGAGATAAATTTTCCAAACAC
  CTGACTCTGTGTGTTTTTACCAACCATACAGGAAGTTTGTGTGTATGTTACAGCCCGAAGTGTGCCTCTT
  GGGGACAAATCACATATTCAGTGTTTTACATTCATAAAGGACACAGCAAGACCTTCACCACTTCTCTTGA
  GAATGTTGGGTCACACATGACAAAGGGCATTACTTTTCTCAACCTTGACTATTATGTGGCTGTTTACTTA
  CCTGGTCATTTCTTCCACCTACTTAATGTTCAACATCCAGACCTGATCTGCCACAATCTCTTTCTGACAG
  GAAATAATGAAATGATTGATATGCTACCTCATTGCCCTTTACAGTCATTGTCAGGGTCCCTGGTATTGGA
  TTGTTGTTCTGGAAAGCTCTATAGAGCACTGCTCAGCCAGTCGTCTTTATTACAGCTTCTGCAGAACACT
  TGCTTAGACTGTGAGAAGATGGCTGCGTTGCACTGCGCGCTCTACTGCGGTCAAGGTGCGCAGTTCCTGG
  AAGCCCAGATTATTCAGTGGATTTCTGAGAATGTCTCTGCCTGCCATTCATTTGACCTCATTCAGGAATT
  TATAATTGCTTCTTCATACTGGAGTGTATATTCAGAGACAAGTAACATGGACAAACTATTGCCACATTCC
  TCAGTGCTCACTTGGAATACAGAAATTCCTGGAATAACTCTTGTGACAGAAGACATTGCATTGCCTCTTA
  TGAAGGTGCTCAGCTTTAAGGGCTACTGGGAAAAACTGAACTCCAACCTAGAATATGTTAAGTACGCCAA
  GCCACACTTCCACTATAACAACAGTGTGGTCAGGAGAGAGTGGCACAACCTGATCTCTGAAGAGAAAACA
  GGAAAAAGAAGGTCTGCGGCATACGTGAGGAATATTCTTGATAATGCAGTAAAGGTGATTTCTAACCTAG
  AAGCAAGAAATTTGGGGCCAAGATTAACACCCCTCCTGCAGGAGGAAGACAGCCACCAGCGGCTGCTCAT
  GGGGCTGATGGTGTCTGAGCTAAAAGACCATTTTTTGAGACACCTACAGGGTGTAGAAAAGAAGAAAATT
  GAACAGATGGTTCTGGACTACATTTCAAAACTGCTGGATCTCATTTGCCACATCGTAGAAACCAATTTGA
  GGAAACATAATCTTCATTCCTGGGTTCTCCACTTCAATAGTCGTGGCAGTGCTGCTGAATTTGCAGTTTT
  TCACATCATGACCAGGATTCTGGAAGCTACAAACAGTTTGTTTTTACCTCTGCCTCCTGGTTTTCATACT
  CTGCACACCATCCTCGGGGTCCAGTGTCTCCCTTTGCATAACCTGCTGCATTGCATTGACAGTGGAGTGT
  TGCTTCTCACTGAAACAGCTGTCATAAGGCTCATGAAAGATCTGGATAATACAGAGAAAAATGAAAAACT
  GAAATTCAGTATCATTGTGCGGCTTCCTCCGCTTATTGGGCAGAAGATTTGTAGACTTTGGGATCATCCT
  ATGAGTTCTAACATCATTTCGCGGAACCACGTGACGCGACTGCTTCAGAACTATAAGAAACAGCCTCGGA
  ATTCTATGATTAACAAGTCATCGTTCAGTGTAGAATTTCTGCCTCTGAACTACTTCATTGAAATTCTGAC
  AGATATAGAGTCCTCCAATCAAGCCCTGTATCCTTTTGAAGGACATGACAATGTGGATGCAGAATTTGTA
  GAGGAAGCAGCTCTGAAACACACCGCGATGCTTTTAGGCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222360 representing NM_017439
  Red=Cloning site Green=Tags(s)
  
  MALRLVADFDLGKDVLPWLRAQRAVSEASGAGSGGADVLENDYESLHVLNVERNGNIIYTYKDDKGNVVF
  GLYDCQTRQNELLYTFEKDLQVFSCSVNSERTLLAASLVQSTKEGKRNELQPGSKCLTLLVEIHPVNNVK
  VLKAVDSYIWVQFLYPHIESHPLPENHLLLISEEKYIEQFRIHVAQEDGNRVVIKNSGHLPRDRIAEDFV
  WAQWDMSEQRLYYIDLKKSRSILKCIQFYADESYNLMFEVPLDISLSNSGFKLVNFGCDYHQYRDKFSKH
  LTLCVFTNHTGSLCVCYSPKCASWGQITYSVFYIHKGHSKTFTTSLENVGSHMTKGITFLNLDYYVAVYL
  PGHFFHLLNVQHPDLICHNLFLTGNNEMIDMLPHCPLQSLSGSLVLDCCSGKLYRALLSQSSLLQLLQNT
  CLDCEKMAALHCALYCGQGAQFLEAQIIQWISENVSACHSFDLIQEFIIASSYWSVYSETSNMDKLLPHS
  SVLTWNTEIPGITLVTEDIALPLMKVLSFKGYWEKLNSNLEYVKYAKPHFHYNNSVVRREWHNLISEEKT
  GKRRSAAYVRNILDNAVKVISNLEARNLGPRLTPLLQEEDSHQRLLMGLMVSELKDHFLRHLQGVEKKKI
  EQMVLDYISKLLDLICHIVETNLRKHNLHSWVLHFNSRGSAAEFAVFHIMTRILEATNSLFLPLPPGFHT
  LHTILGVQCLPLHNLLHCIDSGVLLLTETAVIRLMKDLDNTEKNEKLKFSIIVRLPPLIGQKICRLWDHP
  MSSNIISRNHVTRLLQNYKKQPRNSMINKSSFSVEFLPLNYFIEILTDIESSNQALYPFEGHDNVDAEFV
  EEAALKHTAMLLGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017439
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017439.3, NP_059135.2
• RefSeq Size: 3265 bp
• RefSeq ORF: 2565 bp
• Locus ID: 54103
• UniProt ID: A4D1B5
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome
• MW: 97.6 kDa
• Gene Summary: Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]