SLCO1B3 (NM_019844) Human Tagged ORF Clone

CAT#: RC222317

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SLCO1B3 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 1B3 (SLCO1B3)



  "NM_019844" in other vectors (6)

Reconstitution Protocol

USD 964.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLCO1B3
Synonyms HBLRR; LST-2; LST-3TM13; LST3; OATP-8; OATP1B3; OATP8; SLC21A8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222317 representing NM_019844
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCAACATCAACATTTGAATAAAACAGCAGAGTCAGCATCTTCAGAGAAAAAGAAAACAAGACGCT
GCAATGGATTCAAGATGTTCTTGGCAGCCCTGTCATTCAGCTATATTGCTAAAGCACTAGGTGGAATCAT
TATGAAAATTTCCATCACTCAAATAGAAAGGAGATTTGACATATCCTCTTCTCTTGCTGGTTTAATTGAT
GGAAGCTTTGAAATTGGAAATTTGCTTGTGATTGTATTTGTAAGTTACTTTGGATCTAAACTACACAGAC
CGAAGTTAATTGGAATTGGTTGTCTCCTTATGGGAACTGGAAGTATTTTGACAGCTTTACCACATTTCTT
CATGGGATATTATAGGTATTCTAAAGAAACCCATATTAATCCATCAGAAAATTCAACATCAAGTTTATCA
ACCTGTTTAATTAATCAAACCTTATCATTCAATGGAACATCACCTGAGATAGTAGAAAAAGATTGTGTAA
AGGAATCTGGGTCACACATGTGGATCTATGTCTTCATGGGGAATATGCTTCGTGGCATAGGGGAAACCCC
CATAGTACCATTGGGGATTTCATACATTGATGATTTTGCAAAAGAAGGACATTCTTCCTTGTATTTAGGT
AGTTTGAATGCAATAGGAATGATTGGTCCAGTCATTGGCTTTGCACTGGGATCTCTGTTTGCTAAAATAT
ACGTGGATATTGGATATGTAGATCTGAGCACTATCAGAATAACTCCTAAGGACTCTCGTTGGGTTGGAGC
TTGGTGGCTTGGTTTCCTTGTGTCTGGACTATTTTCCATTATTTCTTCCATACCATTTTTTTTCTTGCCG
AAAAATCCAAATAAACCACAAAAAGAAAGAAAAATTTCACTATCATTGCATGTGCTGAAAACAAATGATG
ATAGAAATCAAACAGCTAATTTGACCAACCAAGGAAAAAATGTTACCAAAAATGTGACTGGTTTTTTCCA
GTCTTTGAAAAGCATCCTTACCAATCCCCTGTATGTTATATTTCTGCTTTTGACATTGTTACAAGTAAGC
AGCTTTATTGGTTCTTTTACTTACGTCTTTAAATATATGGAGCAACAGTACGGTCAGTCTGCATCTCATG
CTAACTTTTTGTTGGGAATCATAACCATTCCTACGGTTGCAACTGGAATGTTTTTAGGAGGATTTATCAT
TAAAAAATTCAAATTGTCTTTAGTTGGAATTGCCAAATTTTCATTTCTTACTTCGATGATATCCTTCTTG
TTTCAACTTCTATATTTCCCTCTAATCTGCGAAAGCAAATCAGTTGCCGGCCTAACCTTGACCTATGATG
GAAATAATTCAGTGGCATCTCATGTAGATGTACCACTTTCTTATTGCAACTCAGAGTGCAATTGTGATGA
AAGTCAGTGGGAACCAGTCTGTGGGAACAATGGAATAACTTACCTGTCACCTTGTCTAGCAGGATGCAAA
TCCTCAAGTGGTATTAAAAAGCATACAGTGTTTTATAACTGTAGTTGTGTGGAAGTAACTGGTCTCCAGA
ACAGAAATTACTCAGCGCACTTGGGTGAATGCCCAAGAGATAATACTTGTACAAGGAAATTTTTCATCTA
TGTTGCAATTCAAGTCATAAACTCTTTGTTCTCTGCAACAGGAGGTACCACATTTATCTTGTTGACTGTG
AAGATTGTTCAACCTGAATTGAAAGCACTTGCAATGGGTTTCCAGTCAATGGTTATAAGAACACTAGGAG
GAATTCTAGCTCCAATATATTTTGGGGCTCTGATTGATAAAACATGTATGAAGTGGTCCACCAACAGCTG
TGGAGCACAAGGAGCTTGTAGGATATATAATTCCGTATTTTTTGGAAGGGTCTACTTGGGCTTATCTATA
GCTTTAAGATTCCCAGCACTTGTTTTATATATTGTTTTCATTTTTGCTATGAAGAAAAAATTTCAAGGAA
AAGATACCAAGGCATCGGACAATGAAAGAAAAGTAATGGATGAAGCAAACTTAGAATTCTTAAATAATGG
TGAACATTTTGTACCTTCTGCTGGAACAGATAGTAAAACATGTAATTTGGACATGCAAGACAATGCTGCT
GCCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222317 representing NM_019844
Red=Cloning site Green=Tags(s)

MDQHQHLNKTAESASSEKKKTRRCNGFKMFLAALSFSYIAKALGGIIMKISITQIERRFDISSSLAGLID
GSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCLLMGTGSILTALPHFFMGYYRYSKETHINPSENSTSSLS
TCLINQTLSFNGTSPEIVEKDCVKESGSHMWIYVFMGNMLRGIGETPIVPLGISYIDDFAKEGHSSLYLG
SLNAIGMIGPVIGFALGSLFAKIYVDIGYVDLSTIRITPKDSRWVGAWWLGFLVSGLFSIISSIPFFFLP
KNPNKPQKERKISLSLHVLKTNDDRNQTANLTNQGKNVTKNVTGFFQSLKSILTNPLYVIFLLLTLLQVS
SFIGSFTYVFKYMEQQYGQSASHANFLLGIITIPTVATGMFLGGFIIKKFKLSLVGIAKFSFLTSMISFL
FQLLYFPLICESKSVAGLTLTYDGNNSVASHVDVPLSYCNSECNCDESQWEPVCGNNGITYLSPCLAGCK
SSSGIKKHTVFYNCSCVEVTGLQNRNYSAHLGECPRDNTCTRKFFIYVAIQVINSLFSATGGTTFILLTV
KIVQPELKALAMGFQSMVIRTLGGILAPIYFGALIDKTCMKWSTNSCGAQGACRIYNSVFFGRVYLGLSI
ALRFPALVLYIVFIFAMKKKFQGKDTKASDNERKVMDEANLEFLNNGEHFVPSAGTDSKTCNLDMQDNAA
AN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019844
ORF Size 2106 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019844.4
RefSeq Size 2646 bp
RefSeq ORF 2109 bp
Locus ID 28234
UniProt ID Q9NPD5
Cytogenetics 12p12.2
Domains OATP_N, OATP_C
Protein Families Druggable Genome, Transmembrane
MW 77.2 kDa
Gene Summary This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of endogenous and xenobiotic compounds and plays a critical role in bile acid and bilirubin transport. Mutations in this gene are a cause of Rotor type hyperbilirubinemia. Alternative splicing of this gene and the use of alternative promoters results in transcript variants encoding different isoforms that differ in their tissue specificity. [provided by RefSeq, Mar 2017]

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