Frizzled 8 (FZD8) (NM_031866) Human Tagged Lenti ORF Clone

CAT#: RC222311L3

  • LentiORF®

Lenti ORF clone of Human frizzled family receptor 8 (FZD8), Myc-DDK-tagged


  "NM_031866" in other vectors (6)

Reconstitution Protocol

USD 1,253.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Reverse primer to sequence targets cloned in expression vectors.
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Frizzled 8"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol Frizzled 8
Synonyms FZ-8; hFZ8
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC222311).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031866
ORF Size 2082 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031866.1
RefSeq Size 3195 bp
RefSeq ORF 2085 bp
Locus ID 8325
UniProt ID Q9H461
Cytogenetics 10p11.21
Protein Families Druggable Genome, Transmembrane
Protein Pathways Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
MW 73.1 kDa
Gene Summary This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.