Adenylate kinase 5 (AK5) (NM_174858) Human Tagged ORF Clone

CAT#: RC222241

AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1

Product Images

Circular map for RC222241

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AK5 (Cat# RC222241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5(Cat# TA501660). Positive lysates LY403569 (100ug) and LC403569 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403569) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222241 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AK5 protein (Cat# TP322241). The protein was produced from HEK293T cells transfected with AK5 cDNA clone (Cat# RC222241) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adenylate kinase 5
• Synonyms: AK6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222241 representing NM_174858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACCAACGATGCCAAGGAGTATCTGGCCCGGAGGGAAATCCCTCAGCTTTTTGAGAGCCTTTTGA
  ATGGACTGATGTGTTCTAAGCCCGAAGATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAAGTAAAGGA
  ACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGGAAAAGAAGACCTTACCTCCACTA
  AATGGAGGACAGTCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATCGGC
  GGTATGACCGGCTCCCTCCAATCCATCAATTCTCCATAGAAAGTGACACGGATCTCTCTGAGACTGCGGA
  GTTGATTGAGGAGTATGAGGTTTTTGATCCTACCAGACCTCGACCAAAAATCATTCTTGTTATAGGTGGT
  CCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTGCAGAACGATATGGATTCCAATACATTTCTGTGG
  GAGAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTATTGCCAAGATAAT
  TACAACTGGAGAATTGGCCCCACAGGAAACAACAATTACAGAGATAAAACAAAAATTGATGCAAATACCT
  GATGAAGAGGGCATTGTTATTGATGGATTTCCAAGAGATGTTGCCCAGGCTCTATCTTTTGAGGACCAAA
  TCTGTACCCCCGATTTGGTGGTATTCCTGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTACTGAAGCG
  TGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAAGGAGACTAATGAACTTCAAGCAG
  AATGCTGCTCCATTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCGATG
  AGGATGAGGTGTTCTATGACATCAGCATGGCAGTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGC
  AGGTTCAAGTGACCTTGATCCTTCGATGATATTGGACACTGGAGAGATCATTGATACAGGATCTGATTAT
  GAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAGAGGACACTATGGGAGGTTTCATGGAAGATTTGA
  GAAAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGTGTGAAAAGCTGGT
  GGAAAAATATGGATTTACACATCTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAA
  AGAAGCAAATTGATCAGAGACATTATGGAACGTGGAGACCTGGTGCCCTCAGGCATCGTTTTGGAGCTCC
  TGAAGGAGGCCATGGTGGCCAGCCTCGGGGACACCAGGGGCTTCCTGATTGACGGCTATCCTCGGGAGGT
  GAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGGTGATCTGTATGGACTGCTCGGCA
  GACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCA
  TCGCCAAGCGCCTAGAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACA
  GCTACACAAGATAAATGCAGAGGGAACACCAGAGGACGTTTTTCTTCAACTCTGCACAGCTATTGACTCT
  ATTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222241 representing NM_174858
  Red=Cloning site Green=Tags(s)
  
  MNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPL
  NGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGG
  PGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIP
  DEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQ
  NAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDY
  EDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESE
  RSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSA
  DTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDS
  IF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174858
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174858.3
• RefSeq Size: 3257 bp
• RefSeq ORF: 1689 bp
• Locus ID: 26289
• UniProt ID: Q9Y6K8
• Cytogenetics: 1p31.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 63.2 kDa
• Gene Summary: This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]