Kv1.2 (KCNA2) (NM_004974) Human Tagged ORF Clone
KCNA2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||DEE32; EIEE32; HBK5; HK4; HUKIV; KV1.2; MK2; NGK1; RBK2|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC222200 representing NM_004974
Red=Cloning site Blue=ORF Green=Tags(s)
>RC222200 representing NM_004974
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1497 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2142 bp|
|RefSeq ORF||1500 bp|
|Protein Families||Druggable Genome, Ion Channels: Potassium, Transmembrane|
|Gene Summary||Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC222200L1||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, Myc-DDK-tagged||
|RC222200L2||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, mGFP tagged||
|RC222200L3||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, Myc-DDK-tagged||
|RC222200L4||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, mGFP tagged||
|RG222200||KCNA2 (GFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1||
|SC303558||KCNA2 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1||