Sonic Hedgehog (SHH) (NM_000193) Human Tagged ORF Clone
SHH (Myc-DDK-tagged)-Human sonic hedgehog (SHH)
|Product Name||Sonic Hedgehog (SHH) (NM_000193) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||HHG1; HLP3; HPE3; MCOPCB5; ShhNC; SMMCI; TPT; TPTPS|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC222175 representing NM_000193
Red=Cloning site Blue=ORF Green=Tags(s)
>RC222175 representing NM_000193
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1386 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1577 bp|
|RefSeq ORF||1389 bp|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer|
|Gene Summary||This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Dual roles of the Sterol Recognition Region in Hedgehog protein modification
,Purohit, R;Peng, DS;Vielmas, E;Ondrus, AE;,
Preclinical characterization of therapeutic antibodies targeted at the carboxy-terminus of Sonic hedgehog
,Tolani, B;Hoang, NT;Acevedo, LA;Leprieur, EG;Li, H;He, B;Jablons, DM;,
,PubMed ID 29581846
Membrane-bound full-length Sonic Hedgehog identifies cancer stem cells in human non-small cell lung cancer
,Leprieur, EG;Tolani, B;Li, H;Leguay, F;Hoang, NT;Acevedo, LA;Jin, JQ;Tseng, HH;Yue, D;Kim, IJ;Wislez, M;Wang, C;Jablons, DM;He, B;,
,PubMed ID 29262597
N-docosahexaenoylethanolamine regulates Hedgehog signaling and promotes growth of cortical axons
,Kharebava, G;Rashid, MA;Lee, JW;Sarkar, S;Kevala, K;Kim, HY;,
Biol Open Nov 2015
,PubMed ID 26545965
MicroRNA-602 and microRNA-608 regulate sonic hedgehog expression via target sites in the coding region in human chondrocytes
,Akhtar, N;Makki, MS;Haqqi, TM;,
Arthritis & rheumatology Nov 2014
,PubMed ID 25385442
|cDNA Clone Resources|
|SC300021||SHH (untagged)-Human sonic hedgehog (SHH)||
|RG222175||SHH (GFP-tagged) - Human sonic hedgehog (SHH)||
|RC222175L1||Lenti ORF clone of Human sonic hedgehog (SHH), Myc-DDK-tagged||
|RC222175L2||Lenti ORF clone of Human sonic hedgehog (SHH), mGFP tagged||
|RC222175L3||Lenti ORF clone of Human sonic hedgehog (SHH), Myc-DDK-tagged||
|RC222175L4||Lenti ORF clone of Human sonic hedgehog (SHH), mGFP tagged||