OATP2 (SLCO1B1) (NM_006446) Human Tagged ORF Clone

CAT#: RC222130

SLCO1B1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401938) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLCO1B1
• Synonyms: HBLRR; LST-1; LST1; OATP-C; OATP1B1; OATP2; OATPC; SLC21A6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222130 representing NM_006446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAAAATCAACATTTGAATAAAACAGCAGAGGCACAACCTTCAGAGAATAAGAAAACAAGATACT
  GCAATGGATTGAAGATGTTCTTGGCAGCTCTGTCACTCAGCTTTATTGCTAAGACACTAGGTGCAATTAT
  TATGAAAAGTTCCATCATTCATATAGAACGGAGATTTGAGATATCCTCTTCTCTTGTTGGTTTTATTGAC
  GGAAGCTTTGAAATTGGAAATTTGCTTGTGATTGTATTTGTGAGTTACTTTGGATCCAAACTACATAGAC
  CAAAGTTAATTGGAATCGGTTGTTTCATTATGGGAATTGGAGGTGTTTTGACTGCTTTGCCACATTTCTT
  CATGGGATATTACAGGTATTCTAAAGAAACTAATATCGATTCATCAGAAAATTCAACATCGACCTTATCC
  ACTTGTTTAATTAATCAAATTTTATCACTCAATAGAGCATCACCTGAGATAGTGGGAAAAGGTTGTTTAA
  AGGAATCTGGGTCATACATGTGGATATATGTGTTCATGGGTAATATGCTTCGTGGAATAGGGGAGACTCC
  CATAGTACCATTGGGGCTTTCTTACATTGATGATTTCGCTAAAGAAGGACATTCTTCTTTGTATTTAGGT
  ATATTGAATGCAATAGCAATGATTGGTCCAATCATTGGCTTTACCCTGGGATCTCTGTTTTCTAAAATGT
  ACGTGGATATTGGATATGTAGATCTAAGCACTATCAGGATAACTCCTACTGATTCTCGATGGGTTGGAGC
  TTGGTGGCTTAATTTCCTTGTGTCTGGACTATTCTCCATTATTTCTTCCATACCATTCTTTTTCTTGCCC
  CAAACTCCAAATAAACCACAAAAAGAAAGAAAAGCTTCACTGTCTTTGCATGTGCTGGAAACAAATGATG
  AAAAGGATCAAACAGCTAATTTGACCAATCAAGGAAAAAATATTACCAAAAATGTGACTGGTTTTTTCCA
  GTCTTTTAAAAGCATCCTTACTAATCCCCTGTATGTTATGTTTGTGCTTTTGACGTTGTTACAAGTAAGC
  AGCTATATTGGTGCTTTTACTTATGTCTTCAAATACGTAGAGCAACAGTATGGTCAGCCTTCATCTAAGG
  CTAACATCTTATTGGGAGTCATAACCATACCTATTTTTGCAAGTGGAATGTTTTTAGGAGGATATATCAT
  TAAAAAATTCAAACTGAACACCGTTGGAATTGCCAAATTCTCATGTTTTACTGCTGTGATGTCATTGTCC
  TTTTACCTATTATATTTTTTCATACTCTGTGAAAACAAATCAGTTGCCGGACTAACCATGACCTATGATG
  GAAATAATCCAGTGACATCTCATAGAGATGTACCACTTTCTTATTGCAACTCAGACTGCAATTGTGATGA
  AAGTCAATGGGAACCAGTCTGTGGAAACAATGGAATAACTTACATCTCACCCTGTCTAGCAGGTTGCAAA
  TCTTCAAGTGGCAATAAAAAGCCTATAGTGTTTTACAACTGCAGTTGTTTGGAAGTAACTGGTCTCCAGA
  ACAGAAATTACTCAGCCCATTTGGGTGAATGCCCAAGAGATGATGCTTGTACAAGGAAATTTTACTTTTT
  TGTTGCAATACAAGTCTTGAATTTATTTTTCTCTGCACTTGGAGGCACCTCACATGTCATGCTGATTGTT
  AAAATTGTTCAACCTGAATTGAAATCACTTGCACTGGGTTTCCACTCAATGGTTATACGAGCACTAGGAG
  GAATTCTAGCTCCAATATATTTTGGGGCTCTGATTGATACAACGTGTATAAAGTGGTCCACCAACAACTG
  TGGCACACGTGGGTCATGTAGGACATATAATTCCACATCATTTTCAAGGGTCTACTTGGGCTTGTCTTCA
  ATGTTAAGAGTCTCATCACTTGTTTTATATATTATATTAATTTATGCCATGAAGAAAAAATATCAAGAGA
  AAGATATCAATGCATCAGAAAATGGAAGTGTCATGGATGAAGCAAACTTAGAATCCTTAAATAAAAATAA
  ACATTTTGTCCCTTCTGCTGGGGCAGATAGTGAAACACATTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222130 representing NM_006446
  Red=Cloning site Green=Tags(s)
  
  MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFID
  GSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNIDSSENSTSTLS
  TCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLG
  ILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLP
  QTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVS
  SYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLS
  FYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCK
  SSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIV
  KIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSS
  MLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006446
• ORF Size: 2073 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006446.3
• RefSeq Size: 2830 bp
• RefSeq ORF: 2076 bp
• Locus ID: 10599
• UniProt ID: Q9Y6L6
• Cytogenetics: 12p12.1
• Domains: OATP_N, OATP_C
• Protein Families: Druggable Genome, Transmembrane
• MW: 76.3 kDa
• Gene Summary: This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and leukotriene C4. This protein is also involved in the removal of drug compounds such as statins, bromosulfophthalein and rifampin from the blood into the hepatocytes. Polymorphisms in the gene encoding this protein are associated with impaired transporter function. [provided by RefSeq, Mar 2009]