PIB5PA (INPP5J) (NM_001002837) Human Tagged ORF Clone

CAT#: RC222056

INPP5J (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase J (INPP5J)

Product Images

Circular map for RC222056

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  Western blot validation of overexpression lysate (Cat# LY424163) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222056 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified INPP5J protein (Cat# TP322056). The protein was produced from HEK293T cells transfected with INPP5J cDNA clone (Cat# RC222056) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIB5PA
• Synonyms: INPP5; PIB5PA; PIPP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222056 representing NM_001002837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCCAGAGCAGCAGGGGCAGCAGGAGGCCAGGGACCCGGGCTGGCCTGGGTTCCCTGCCCATGC
  CCCAGGGTGTTGCCCAAACTGGGGCACCCTCCAAGGTGGACTCAAGTTTTCAGCTCCCAGCAAAGAAGAA
  CGCAGCCCTAGGACCCTCGGAACCAAGGATCACTGTGGTCACATGGAACGTGGGCACTGCCATGCCCCCA
  GACGATGTCACATCCCTCCTCCACCTGGGCGGTGGTGACGACAGCGACGGCGCAGACATGATCGCCATAG
  GGTTGCAGGAAGTGAACTCCATGCTCAACAAGCGACTCAAGGACGCCCTCTTCACGGACCAGTGGAGTGA
  GCTGTTCATGGATGCGCTAGGGCCCTTCAACTTCGTGCTGGTGAGTTCGGTGAGGATGCAGGGTGTCATC
  CTGCTGCTGTTCGCCAAGTACTACCACCTGCCCTTCCTGCGAGACGTGCAGACCGACTGCACGCGCACTG
  GCCTGGGCGGCTACTGGGGTAACAAGGGTGGCGTGAGCGTGCGCCTGGCGGCCTTCGGGCACATGCTCTG
  CTTCCTGAACTGCCACTTGCCTGCGCATATGGACAAGGCGGAGCAGCGCAAAGACAACTTCCAGACCATC
  CTCAGCCTCCAGCAGTTCCAAGGGCCGGGCGCACAGGGCATCCTGGATCATGACCTCGTGTTCTGGTTCG
  GGGACCTGAACTTCCGCATTGAGAGCTATGACCTGCACTTTGTCAAGTTTGCCATCGACAGTGACCAGCT
  CCATCAGCTCTGGGAGAAGGACCAGCTCAACATGGCCAAGAACACCTGGCCCATTCTGAAGGGCTTTCAG
  GAGGGGCCCCTCAACTTCGCTCCCACCTTCAAGTTTGATGTGGGTACCAACAAATACGATACCAGTGCCA
  AGAAACGGAAGCCAGCTTGGACAGACCGTATCCTATGGAAGGTCAAGGCTCCAGGTGGGGGTCCCAGCCC
  CTCAGGACGGAAGAGCCACCGACTCCAGGTGACGCAGCACAGCTACCGCAGCCACATGGAATACACAGTC
  AGCGACCACAAGCCTGTGGCTGCCCAGTTCCTCCTGCAGTTTGCCTTCAGGGACGACATGCCACTGGTGC
  GGCTGGAGGTGGCAGATGAGTGGGTGCGGCCCGAGCAGGCGGTGGTGAGGTACCGCATGGAAACAGTGTT
  CGCCCGCAGCTCCTGGGACTGGATCGGCTTATACCGGGTGGGTTTCCGCCATTGCAAGGACTATGTGGCT
  TATGTCCGGGCCAAACATGAAGATGTGGATGGGAATACATACCAGGTAACATTCAGTGAGGAATCACTGC
  CCAAGGGCCATGGAGACTTCATCCTGGGCTACTATAGTCACAACCACAGCATCCTCATCGGCATCACTGA
  ACCCTTCCAGATCTCGCTGCCTTCCTCGGAGTTGGCCAGCAGCAGCACAGACAGCTCAGGCACCAGCTCA
  GAGGGAGAGGATGACAGCACACTGGAGCTCCTTGCACCCAAGTCCCGCAGCCCCAGTCCTGGCAAGTCCA
  AGCGACACCGCAGCCGCAGCCCGGGACTGGCCAGGTTCCCTGGGCTTGCCCTACGGCCCTCATCCCGTGA
  ACGCCGTGGTGCCAGCCGTAGCCCCTCACCCCAGAGCCGCCGCCTGTCCCGAGTGGCTCCTGACAGGAGC
  AGTAATGGCAGCAGCCGGGGCAGTAGTGAAGAGGGGCCCTCTGGGTTGCCTGGCCCCTGGGCCTTCCCAC
  CAGCTGTGCCTCGAAGCCTGGGCCTGTTGCCCGCCTTGCGCCTAGAGACTGTAGACCCTGGTGGTGGTGG
  CTCCTGGGGACCTGATCGGGAGGCCCTGGCGCCCAACAGCCTGTCTCCTAGTCCCCAGGGCCATCGGGGG
  CTGGAGGAAGGGGGCCTGGGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222056 representing NM_001002837
  Red=Cloning site Green=Tags(s)
  
  MEGQSSRGSRRPGTRAGLGSLPMPQGVAQTGAPSKVDSSFQLPAKKNAALGPSEPRITVVTWNVGTAMPP
  DDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFTDQWSELFMDALGPFNFVLVSSVRMQGVI
  LLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQTI
  LSLQQFQGPGAQGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGFQ
  EGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYTV
  SDHKPVAAQFLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGFRHCKDYVA
  YVRAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPFQISLPSSELASSSTDSSGTSS
  EGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPGLALRPSSRERRGASRSPSPQSRRLSRVAPDRS
  SNGSSRGSSEEGPSGLPGPWAFPPAVPRSLGLLPALRLETVDPGGGGSWGPDREALAPNSLSPSPQGHRG
  LEEGGLGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002837
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002837.2, NP_001002837.1
• RefSeq Size: 2238 bp
• RefSeq ORF: 1917 bp
• Locus ID: 27124
• UniProt ID: Q15735
• Cytogenetics: 22q12.2
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 70.1 kDa
• Gene Summary: Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).[UniProtKB/Swiss-Prot Function]