PTPRS (NM_130855) Human Tagged ORF Clone

CAT#: RC221938

PTPRS (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, S (PTPRS), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY408880) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221938 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PTPRS protein (Cat# TP321938). The protein was produced from HEK293T cells transfected with PTPRS cDNA clone (Cat# RC221938) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPRS
• Synonyms: PTPSIGMA; R-PTP-S; R-PTP-sigma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221938 representing NM_130855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCACCTGGGGCCCTGGCATGGTGTCTGTGGTTGGTCCCATGGGCCTCCTTGTGGTCCTGCTCG
  TTGGAGGCTGTGCAGCAGAAGAGCCCCCCAGGTTTATCAAAGAACCCAAGGACCAGATCGGCGTGTCGGG
  GGGTGTGGCCTCTTTCGTGTGTCAGGCCACGGGTGACCCCAAGCCACGAGTGACCTGGAACAAGAAGGGC
  AAGAAGGTCAACTCTCAGCGCTTTGAGACGATTGAGTTTGATGAGAGTGCAGGGGCAGTGCTGAGGATCC
  AGCCGCTGAGGACACCGCGGGATGAAAACGTGTACGAGTGTGTGGCCCAGAACTCGGTTGGGGAGATCAC
  AGTCCATGCCAAGCTTACTGTCCTCCGAGAGGACCAGCTGCCCTCTGGCTTCCCCAACATCGACATGGGC
  CCACAGTTGAAGGTGGTGGAGCGGACACGGACAGCCACCATGCTCTGTGCAGCCAGCGGCAACCCTGACC
  CTGAGATCACCTGGTTCAAGGACTTCCTGCCTGTGGATCCTAGTGCCAGCAATGGACGCATCAAACAGCT
  GCGATCAGGAGCCCTGCAGATTGAAAGCAGTGAGGAAACCGACCAGGGCAAATATGAGTGTGTGGCCACC
  AACAGCGCCGGCGTGCGCTACTCCTCACCTGCCAACCTCTACGTGCGAGAGCTTCGAGAAGTCCGCCGCG
  TGGCCCCGCGCTTCTCCATCCTGCCCATGAGCCACGAGATCATGCCAGGGGGCAACGTGAACATCACCTG
  CGTGGCCGTGGGCTCGCCCATGCCATACGTGAAGTGGATGCAGGGGGCCGAGGACCTGACCCCCGAGGAT
  GACATGCCCGTGGGTCGGAACGTGCTGGAACTCACAGATGTCAAGGACTCGGCCAACTACACCTGCGTGG
  CCATGTCCAGCCTGGGCGTCATTGAGGCGGTTGCTCAGATCACGGTGAAATCTCTCCCCAAAGCTCCCGG
  GACTCCCATGGTGACTGAGAACACAGCCACCAGCATCACCATCACGTGGGACTCGGGCAACCCAGATCCT
  GTGTCCTATTACGTCATCGAATATAAATCCAAGAGCCAAGACGGGCCGTATCAGATTAAAGAGGACATCA
  CCACCACACGTTACAGCATCGGCGGCCTGAGCCCCAACTCGGAGTACGAGATCTGGGTGTCGGCCGTCAA
  CTCCATCGGCCAGGGGCCCCCCAGCGAGTCCGTGGTCACCCGCACAGGCGAGCAGGCCCCGGCCAGCGCG
  CCGCGGAACGTGCAAGCCCGGATGCTCAGCGCGACCACCATGATTGTGCAGTGGGAGGAGCCGGTGGAGC
  CCAACGGCCTGATCCGCGGCTACCGCGTCTACTACACCATGGAACCGGAGCACCCCGTGGGCAACTGGCA
  GAAGCACAACGTGGACGACAGCCTGCTGACCACCGTGGGCAGCCTGCTGGAGGACGAGACCTACACCGTG
  CGGGTGCTCGCCTTCACCTCCGTCGGCGACGGGCCCCTCTCGGACCCCATCCAGGTCAAGACGCAGCAGG
  GAGTGCCGGGCCAGCCCATGAACCTGCGGGCCGAGGCCAGGTCGGAGACCAGCATCACGCTGTCCTGGAG
  CCCCCCGCGGCAGGAGAGTATCATCAAGTACGAGCTCCTCTTCCGGGAAGGCGACCATGGCCGGGAGGTG
  GGAAGGACCTTCGACCCGACGACTTCCTACGTGGTGGAGGACCTGAAGCCCAACACGGAGTACGCCTTCC
  GCCTGGCGGCCCGCTCGCCGCAGGGCCTGGGCGCCTTCACCCCCGTGGTGCGGCAGCGCACGCTGCAGTC
  CATCTCGCCCAAGAACTTCAAGGTGAAAATGATCATGAAGACATCAGTTCTGCTCAGCTGGGAGTTCCCT
  GACAACTACAACTCACCCACACCCTACAAGATCCAGTACAATGGGCTCACACTGGATGTGGATGGCCGTA
  CCACCAAGAAGCTCATCACGCACCTCAAGCCCCACACCTTCTACAACTTTGTGCTGACCAATCGCGGCAG
  CAGCCTGGGCGGCCTCCAGCAGACGGTCACCGCCTGGACTGCCTTCAACCTGCTCAACGGCAAGCCCAGC
  GTCGCCCCCAAGCCTGATGCTGACGGCTTCATCATGGTGTATCTTCCTGACGGCCAGAGCCCCGTGCCTG
  TCCAGAGCTATTTCATTGTGATGGTGCCACTGCGCAAGTCTCGTGGAGGCCAATTCCTGACCCCGCTGGG
  TAGCCCAGAGGACATGGATCTGGAAGAGCTCATCCAGGACATCTCACGGCTACAGAGGCGCAGCCTGCGG
  CACTCGCGTCAGCTGGAGGTGCCCCGGCCCTATATTGCAGCTCGCTTCTCTGTGCTGCCACCCACGTTCC
  ATCCCGGCGACCAGAAGCAGTATGGCGGCTTCGATAACCGGGGCCTGGAGCCCGGCCACCGCTATGTCCT
  CTTCGTGCTTGCCGTGCTTCAGAAGAGCGAGCCTACCTTTGCAGCCAGTCCCTTCTCAGACCCCTTCCAG
  CTGGATAACCCGGACCCCCAGCCCATCGTGGATGGCGAGGAGGGGCTTATCTGGGTGATCGGGCCTGTGC
  TGGCCGTGGTCTTCATAATCTGCATTGTCATTGCTATCCTGCTCTACAAGAACAAACCCGACAGTAAACG
  CAAGGACTCAGAACCCCGCACCAAATGCCTCCTGAACAATGCCGACCTCGCCCCTCACCACCCCAAGGAC
  CCTGTGGAAATGAGACGCATTAACTTCCAGACTCCAGGCATGCTTAGCCACCCGCCAATTCCCATCGCAG
  ACATGGCGGAGCACACGGAGCGGCTCAAGGCCAACGACAGCCTCAAGCTCTCCCAGGAGTATGAGTCCAT
  CGACCCTGGACAGCAGTTCACATGGGAACATTCCAACCTGGAAGTGAACAAGCCGAAGAACCGCTATGCC
  AACGTCATCGCCTATGACCACTCCCGTGTCATCCTCCAGCCCATTGAAGGCATCATGGGCAGTGATTACA
  TCAATGCCAACTACGTGGACGGCTACCGGTGTCAGAACGCGTACATTGCCACGCAGGGGCCGCTGCCTGA
  GACCTTTGGGGACTTCTGGCGTATGGTGTGGGAGCAGCGGTCGGCGACCATCGTCATGATGACGCGGCTG
  GAGGAGAAGTCACGGATCAAGTGTGATCAGTATTGGCCCAACAGAGGCACGGAGACCTACGGCTTCATCC
  AGGTCACGTTGCTAGATACCATCGAGCTGGCCACATTCTGCGTCAGGACATTCTCTCTGCACAAGAATGG
  CTCCAGTGAGAAACGCGAGGTCCGCCAGTTCCAGTTTACGGCGTGGCCGGACCATGGCGTGCCCGAATAC
  CCAACGCCCTTCCTGGCTTTCCTGCGGAGAGTCAAGACCTGCAACCCGCCAGATGCCGGCCCCATCGTGG
  TTCACTGCAGTGCCGGTGTGGGCCGCACAGGCTGCTTTATCGTCATCGACGCCATGCTTGAGCGGATCAA
  GCCAGAGAAGACAGTCGATGTCTATGGCCACGTGACGCTCATGAGGTCCCAGCGCAACTACATGGTGCAG
  ACGGAGGACCAGTACAGCTTCATCCACGAGGCCCTGCTGGAGGCCGTGGGCTGTGGCAACACAGAAGTGC
  CCGCACGCAGCCTCTATGCCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAACACGTCACTGGCAT
  GGAACTCGAGTTCAAGCGGCTGGCTAACTCCAAGGCCCACACGTCACGCTTCATCAGTGCCAATCTGCCT
  TGTAACAAGTTCAAGAACCGCCTGGTGAACATCATGCCCTATGAGAGCACACGGGTCTGTCTGCAACCCA
  TCCGGGGTGTGGAGGGCTCTGACTACATCAACGCCAGCTTCATTGATGGCTACAGGCAGCAGAAGGCCTA
  CATCGCGACACAGGGGCCGCTGGCGGAGACCACGGAAGACTTCTGGCGCATGCTGTGGGAGAACAATTCG
  ACGATCGTGGTGATGCTGACCAAGCTGCGGGAGATGGGCCGGGAGAAGTGTCACCAGTACTGGCCGGCCG
  AGCGCTCTGCCCGCTACCAGTACTTTGTGGTAGATCCGATGGCAGAATACAACATGCCTCAGTATATCCT
  GCGAGAGTTCAAGGTCACAGATGCCCGGGATGGCCAGTCCCGGACTGTCCGGCAGTTCCAGTTCACAGAC
  TGGCCGGAACAGGGTGTGCCAAAGTCGGGGGAGGGCTTCATCGACTTCATTGGCCAAGTGCATAAGACTA
  AGGAGCAGTTTGGCCAGGACGGCCCCATCTCTGTCCACTGCAGTGCCGGCGTGGGCAGGACGGGCGTCTT
  CATCACGCTTAGCATCGTGCTGGAGCGGATGCGGTATGAAGGCGTGGTGGACATCTTTCAGACGGTGAAG
  ATGCTACGAACCCAGCGGCCGGCCATGGTGCAGACAGAGGATGAGTACCAGTTCTGTTACCAGGCGGCAC
  TGGAGTACCTCGGAAGCTTTGACCACTATGCAACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221938 representing NM_130855
  Red=Cloning site Green=Tags(s)
  
  MAPTWGPGMVSVVGPMGLLVVLLVGGCAAEEPPRFIKEPKDQIGVSGGVASFVCQATGDPKPRVTWNKKG
  KKVNSQRFETIEFDESAGAVLRIQPLRTPRDENVYECVAQNSVGEITVHAKLTVLREDQLPSGFPNIDMG
  PQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSGALQIESSEETDQGKYECVAT
  NSAGVRYSSPANLYVRELREVRRVAPRFSILPMSHEIMPGGNVNITCVAVGSPMPYVKWMQGAEDLTPED
  DMPVGRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSLPKAPGTPMVTENTATSITITWDSGNPDP
  VSYYVIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNSIGQGPPSESVVTRTGEQAPASA
  PRNVQARMLSATTMIVQWEEPVEPNGLIRGYRVYYTMEPEHPVGNWQKHNVDDSLLTTVGSLLEDETYTV
  RVLAFTSVGDGPLSDPIQVKTQQGVPGQPMNLRAEARSETSITLSWSPPRQESIIKYELLFREGDHGREV
  GRTFDPTTSYVVEDLKPNTEYAFRLAARSPQGLGAFTPVVRQRTLQSISPKNFKVKMIMKTSVLLSWEFP
  DNYNSPTPYKIQYNGLTLDVDGRTTKKLITHLKPHTFYNFVLTNRGSSLGGLQQTVTAWTAFNLLNGKPS
  VAPKPDADGFIMVYLPDGQSPVPVQSYFIVMVPLRKSRGGQFLTPLGSPEDMDLEELIQDISRLQRRSLR
  HSRQLEVPRPYIAARFSVLPPTFHPGDQKQYGGFDNRGLEPGHRYVLFVLAVLQKSEPTFAASPFSDPFQ
  LDNPDPQPIVDGEEGLIWVIGPVLAVVFIICIVIAILLYKNKPDSKRKDSEPRTKCLLNNADLAPHHPKD
  PVEMRRINFQTPGMLSHPPIPIADMAEHTERLKANDSLKLSQEYESIDPGQQFTWEHSNLEVNKPKNRYA
  NVIAYDHSRVILQPIEGIMGSDYINANYVDGYRCQNAYIATQGPLPETFGDFWRMVWEQRSATIVMMTRL
  EEKSRIKCDQYWPNRGTETYGFIQVTLLDTIELATFCVRTFSLHKNGSSEKREVRQFQFTAWPDHGVPEY
  PTPFLAFLRRVKTCNPPDAGPIVVHCSAGVGRTGCFIVIDAMLERIKPEKTVDVYGHVTLMRSQRNYMVQ
  TEDQYSFIHEALLEAVGCGNTEVPARSLYAYIQKLAQVEPGEHVTGMELEFKRLANSKAHTSRFISANLP
  CNKFKNRLVNIMPYESTRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRMLWENNS
  TIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTD
  WPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVK
  MLRTQRPAMVQTEDEYQFCYQAALEYLGSFDHYAT
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130855
• ORF Size: 4515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130855.3
• RefSeq Size: 5171 bp
• RefSeq ORF: 4518 bp
• Locus ID: 5802
• UniProt ID: Q13332
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• MW: 166.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]