ANKHD1 (NM_017978) Human Tagged ORF Clone

CAT#: RC221886

ANKHD1 (Myc-DDK-tagged)-Human ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY413410) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221886 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKHD1
• Synonyms: MASK; MASK1; PP2500; VBARP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221886 representing NM_017978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACTGATAGCGGAGGCGGCGGCACCTCCTTTGAGGAGGACCTGGACTCTGTGGCTCCGCGATCCG
  CCCCAGCTGGGGCCTCGGAGCCGCCTCCGCCGGGAGGGGTCGGTCTGGGGATCCGCACCGTGAGGCTCTT
  TGGGGAGGCCGGGCCAGCGTCGGGAGTCGGCAGCAGCGGCGGCGGCGGCAGCGGCAGCGGTACGGGCGGA
  GGGGACGCGGCGCTGGATTTCAAGTTGGCGGCTGCCGTGCTGAGGACCGGGGGTGGAGGTGGTGCCTCTG
  GCAGTGACGAGGACGAAGTGTCCGAGGTTGAATCATTTATTTTGGACCAAGAAGATCTGGATAACCCAGT
  GCTTAAAACAACATCAGAGATATTCTTATCAAGTACTGCAGAAGGAGCAGACTTACGCACTGTGGATCCA
  GAGACACAGGCACGACTAGAAGCATTGCTAGAAGCAGCAGCTTTTGCAGATCCTGAGGTACTCCGGAGAC
  TGACATCCTCAGTTAGTTGTGCACTGGATGAAGCTGCTGCTGCACTGACACGGATGAAAGCAGAAAACAG
  CCACAATGCAGGACAAGTGGACACTCGCAGTCTAGCAGAAGCTTGTTCAGATGGGGATGTTAATGCTGTT
  CGTAAATTGCTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGCCTGCTGTGTTTGG
  CTTGTTCAGCAGGGTATTATGAATTAGCACAAGTATTGCTTGCTATGCATGCTAATGTTGAAGATCGAGG
  GAATAAAGGAGACATAACTCCCCTGATGGCAGCTTCCAGTGGAGGTTACTTAGATATTGTGAAATTATTA
  CTTCTTCATGATGCTGATGTCAACTCCCAGTCTGCAACAGGAAACACTGCGCTAACTTATGCATGTGCTG
  GAGGATTTGTTGACATTGTTAAAGTGCTCCTTAATGAAGGTGCAAATATAGAAGATCATAATGAAAATGG
  ACATACTCCCTTAATGGAAGCAGCCAGTGCAGGTCATGTGGAAGTTGCAAGAGTTCTTTTAGATCATGGT
  GCAGGCATCAACACTCATTCTAATGAATTCAAAGAAAGTGCTCTAACACTTGCTTGCTACAAAGGCCATT
  TGGATATGGTTCGCTTTCTACTTGAAGCTGGTGCAGATCAAGAGCACAAAACAGATGAGATGCACACTGC
  CTTAATGGAGGCCTGCATGGATGGACATGTAGAGGTGGCACGTTTGCTTTTGGATAGTGGTGCTCAAGTG
  AACATGCCTGCAGATTCATTTGAATCTCCATTGACGCTAGCTGCCTGTGGAGGACATGTTGAATTGGCAG
  CTCTACTTATTGAAAGGGGAGCAAATCTTGAAGAAGTTAATGATGAAGGATACACTCCCTTGATGGAAGC
  TGCCCGGGAAGGACATGAAGAAATGGTGGCACTACTCTTAGCACAAGGAGCAAATATAAATGCCCAGACA
  GAAGAAACTCAAGAAACTGCTCTTACTTTGGCTTGCTGTGGAGGATTTTCTGAAGTTGCAGACTTTCTTA
  TTAAGGCAGGGGCTGATATAGAACTTGGCTGCTCCACACCTCTGATGGAGGCATCTCAGGAGGGACACCT
  GGAATTGGTTAAATATTTGCTGGCTTCTGGCGCTAATGTGCATGCTACAACAGCAACAGGAGACACAGCC
  TTAACCTATGCTTGTGAAAATGGACATACGGATGTTGCAGATGTTTTACTTCAAGCAGGGGCTGATTTAG
  ACAAGCAGGAGGACATGAAGACTATTTTGGAGGGCATAGATCCGGCCAAGCATCAGGTGAGGGTGGCCTT
  TGATGCTTGTAAGCTACTACGTAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221886 representing NM_017978
  Red=Cloning site Green=Tags(s)
  
  MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGG
  GDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDP
  ETQARLEALLEAAAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAV
  RKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLL
  LLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHG
  AGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQV
  NMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQT
  EETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTA
  LTYACENGHTDVADVLLQAGADLDKQEDMKTILEGIDPAKHQVRVAFDACKLLRKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017978
• ORF Size: 1848 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017978.3
• RefSeq Size: 2149 bp
• RefSeq ORF: 1851 bp
• Locus ID: 54882
• UniProt ID: Q8IWZ3
• Cytogenetics: 5q31.3
• MW: 63.7 kDa
• Gene Summary: This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010]