ZAR1 (NM_175619) Human Tagged ORF Clone

CAT#: RC221731

ZAR1 (Myc-DDK-tagged)-Human zygote arrest 1 (ZAR1)

Product Images

Circular map for RC221731

• 

  Western blot validation of overexpression lysate (Cat# LY406271) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221731 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ZAR1 protein (Cat# TP321731). The protein was produced from HEK293T cells transfected with ZAR1 cDNA clone (Cat# RC221731) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZAR1
• Synonyms: Z3CXXC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221731 representing NM_175619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGGGGGACGAGGTGCTGGACGGTTACGTGTTCCCGGCGTGCCCCCCCTGCTCGTACCGGT
  ACCCATACCCCGCGGCCACCAAGGGCAAGGGCGCGGCGGGCGGCAGCTGGCAGCAGCGCGGCAGGGGCTG
  CCTTCCCGCCTCCTCCCCCTGCTCGGCGGGCGCGGCCTCGTTGTCCTTCCCGGGCTGCGGGCGGCTGACG
  GCCGCCGAGTACTTCGACAGCTACCAGCGGGAGCGGCTCATGGCTCTCCTGGCGCAGGTGGGGCCGGGTC
  TCGGGCCGCGCGCCCGCAGGGCCGGCAGCTGCGACGTGGCGGTGCAGGTGAGCCCGCGCATCGACGCCGC
  GGTACAGTGCTCGCTGGGGAGGCGCACGCTGCAGCGCCGGGCCCGCGACCCCGAGTCCCCGGCCGGCCCC
  GGGGCCGAGGGCACCACGGGTGGCGGCTCTTTCTCCCAGCAGCCATCCCGTCGAGGCCTGGAGCAGGGCA
  GCCCCCAGAACGGCGCCCCGCGGCCCATGCGCTTCCCGCGCACCGTCGCCGTGTACTCGCCCCTGGCCTT
  GCGCCGTCTCACCGCCTTCCTGGAGGGGCCCGGGCCCGCGGCGGGCGAGCAGAGGTCCGGGGCGTCGGAC
  GGAGAGAGGGGGCCGCCGCCCGCGCGGCTTCAAGGCCCAGAGGAGGGGGAGGTGTGGACGAAGAAGGCGC
  CCCGGCGGCCGCAGTCCGACGACGACGGCGAGGCCCAGGCCGCAGTCCGAGCGAGCTGGGAGCAGCCGGC
  CGACGGTCCCGAGCTGCCGCCGCGAGAGGCCCAGGAGGGCGAGGCGGCTCCGCGGTCGGCGCTAAGGAGC
  CCGGGGCAACCTCCGTCGGCGGGGAGGGCCCGAGACGGCGGCGACGGACGGGAGGCGGCCGTCGCGGGAG
  AGGGGCCGTCGCCACGGAGCCCGGAGCTGGGCAAGGAGCGGCTGCGCTTCCAGTTCTTAGAGCAGAAATA
  TGGCTATTACCACTGCAAGGACTGCAACATCCGCTGGGAGAGTGCTTATGTGTGGTGTGTACAGGGAACT
  AACAAGGTTTACTTCAAACAGTTTTGCAGAACTTGTCAGAAGTCTTATAACCCTTACCGAGTGGAGGATA
  TCACCTGTCAAAGTTGTAAACAAACGAGATGTTCCTGCCCAGTAAAACTTCGCCACGTGGACCCTAAACG
  GCCCCACCGTCAAGATTTGTGCGGTAGATGCAAAGGCAAACGCCTGTCCTGTGACAGCACTTTCAGCTTC
  AAATACATCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221731 representing NM_175619
  Red=Cloning site Green=Tags(s)
  
  MAALGDEVLDGYVFPACPPCSYRYPYPAATKGKGAAGGSWQQRGRGCLPASSPCSAGAASLSFPGCGRLT
  AAEYFDSYQRERLMALLAQVGPGLGPRARRAGSCDVAVQVSPRIDAAVQCSLGRRTLQRRARDPESPAGP
  GAEGTTGGGSFSQQPSRRGLEQGSPQNGAPRPMRFPRTVAVYSPLALRRLTAFLEGPGPAAGEQRSGASD
  GERGPPPARLQGPEEGEVWTKKAPRRPQSDDDGEAQAAVRASWEQPADGPELPPREAQEGEAAPRSALRS
  PGQPPSAGRARDGGDGREAAVAGEGPSPRSPELGKERLRFQFLEQKYGYYHCKDCNIRWESAYVWCVQGT
  NKVYFKQFCRTCQKSYNPYRVEDITCQSCKQTRCSCPVKLRHVDPKRPHRQDLCGRCKGKRLSCDSTFSF
  KYII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175619
• ORF Size: 1272 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175619.3
• RefSeq Size: 1438 bp
• RefSeq ORF: 1275 bp
• Locus ID: 326340
• UniProt ID: Q86SH2
• Cytogenetics: 4p11
• MW: 45.7 kDa
• Gene Summary: This maternal effect gene is oocyte-specific and encodes a protein that is thought to function in the initiation of embryogenesis. A similar protein in mouse is required for female fertility. [provided by RefSeq, Jul 2013]