OVGP1 (NM_002557) Human Tagged ORF Clone

CAT#: RC221684

OVGP1 (Myc-DDK-tagged)-Human oviductal glycoprotein 1, 120kDa (OVGP1)

Product Images

Circular map for RC221684

• 

  Western blot validation of overexpression lysate (Cat# LY419261) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221684 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OVGP1 protein (Cat# TP321684). The protein was produced from HEK293T cells transfected with OVGP1 cDNA clone (Cat# RC221684) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OVGP1
• Synonyms: CHIT5; EGP; MUC9; OGP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221684 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGCTGTTGCTGTGGGTTGGGCTGGTTCTTGTGCTGAAACACCACGATGGTGCTGCCCATAAAC
  TCGTGTGTTATTTCACCAACTGGGCACACAGTCGGCCAGGCCCTGCCTCGATCTTGCCCCATGACCTGGA
  CCCCTTTCTCTGCACCCACCTGATATTTGCCTTTGCCTCAATGAACAACAATCAGATTGTTGCTAAGGAT
  CTCCAGGATGAGAAAATTCTCTACCCAGAGTTCAACAAACTAAAGGAGAGGAACAGAGAGCTGAAAACAC
  TACTGTCCATCGGCGGGTGGAACTTTGGCACCTCAAGATTCACCACTATGTTGTCCACATTTGCCAACCG
  TGAAAAGTTTATTGCTTCAGTTATATCCCTTCTGAGGACACATGACTTTGATGGTCTTGACCTTTTCTTC
  TTATATCCTGGACTAAGAGGCAGCCCCATGCATGACCGGTGGACTTTTCTCTTCTTAATTGAAGAGCTCC
  TGTTTGCCTTCCGGAAGGAGGCACTGCTCACCATGCGCCCGAGGCTGCTGCTGTCTGCTGCTGTTTCTGG
  GGTCCCACACATCGTCCAAACATCCTATGATGTGCGCTTTCTAGGAAGACTCCTGGATTTCATCAATGTC
  TTGTCTTATGACTTACATGGAAGTTGGGAAAGGTTCACAGGACATAATAGCCCCCTCTTCTCTCTGCCTG
  AAGACCCCAAATCTTCGGCATATGCTATGAATTATTGGAGAAAGCTTGGGGCACCCTCAGAGAAGCTCAT
  CATGGGGATCCCCACCTATGGACGTACCTTTCGCCTCCTCAAAGCCTCTAAGAATGGGTTGCAGGCCAGA
  GCGATCGGACCAGCATCTCCAGGGAAGTACACCAAGCAAGAAGGCTTCTTGGCTTATTTTGAGATTTGTT
  CCTTTGTCTGGGGAGCGAAGAAGCACTGGATTGATTACCAGTATGTCCCGTATGCCAACAAGGGGAAAGA
  GTGGGTTGGCTATGACAATGCCATCAGCTTCAGTTACAAGGCATGGTTTATAAGGCGAGAGCATTTTGGG
  GGGGCCATGGTGTGGACATTGGACATGGATGACGTCAGGGGCACGTTCTGTGGCACTGGCCCTTTCCCCC
  TTGTCTACGTATTGAATGATATCCTGGTGCGGGCTGAGTTCAGTTCAACTTCTTTACCACAATTTTGGCT
  GTCATCTGCTGTGAATTCTTCAAGCACTGACCCTGAAAGGCTGGCTGTGACCACGGCATGGACCACTGAT
  AGTAAGATTTTGCCCCCAGGAGGAGAGGCTGGGGTCACTGAGATCCACGGAAAGTGTGAAAATATGACTA
  TAACCCCTAGAGGTACAACTGTGACCCCTACAAAGGAAACTGTATCCCTTGGAAAGCACACTGTAGCTCT
  AGGAGAGAAGACTGAGATCACTGGGGCAATGACCATGACTTCTGTGGGTCATCAGTCCATGACCCCTGGA
  GAGAAGGCCCTGACCCCTGTGGGTCATCAATCTGTGACCACTGGACAGAAGACCCTGACCTCTGTGGGTT
  ATCAGTCTGTGACCCCTGGGGAAAAGACCCTGACCCCTGTGGGTCATCAGTCTGTGACCCCTGTGAGTCA
  TCAGTCTGTGAGCCCTGGAGGAACGACTATGACCCCTGTCCATTTTCAGACTGAGACCCTTAGACAGAAT
  ACAGTGGCCCCTAGAAGGAAGGCTGTGGCCCGTGAAAAGGTGACTGTCCCCTCCAGAAACATATCAGTCA
  CCCCTGAAGGGCAGACTATGCCTTTAAGAGGGGAGAATTTGACTTCTGAGGTGGGCACTCACCCCAGGAT
  GGGTAACTTGGGTCTTCAGATGGAAGCTGAAAACAGGATGATGCTGTCCTCCAGCCCCGTCATCCAGCTC
  CCGGAACAAACTCCTCTAGCTTTTGACAACCGCTTTGTTCCCATCTATGGAAACCATTCCTCTGTCAACT
  CAGTAACCCCTCAAACAAGTCCTCTTTCTCTAAAAAAAGAAATCCCAGAAAACTCTGCTGTGGATGAAGA
  AGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221684 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWKLLLWVGLVLVLKHHDGAAHKLVCYFTNWAHSRPGPASILPHDLDPFLCTHLIFAFASMNNNQIVAKD
  LQDEKILYPEFNKLKERNRELKTLLSIGGWNFGTSRFTTMLSTFANREKFIASVISLLRTHDFDGLDLFF
  LYPGLRGSPMHDRWTFLFLIEELLFAFRKEALLTMRPRLLLSAAVSGVPHIVQTSYDVRFLGRLLDFINV
  LSYDLHGSWERFTGHNSPLFSLPEDPKSSAYAMNYWRKLGAPSEKLIMGIPTYGRTFRLLKASKNGLQAR
  AIGPASPGKYTKQEGFLAYFEICSFVWGAKKHWIDYQYVPYANKGKEWVGYDNAISFSYKAWFIRREHFG
  GAMVWTLDMDDVRGTFCGTGPFPLVYVLNDILVRAEFSSTSLPQFWLSSAVNSSSTDPERLAVTTAWTTD
  SKILPPGGEAGVTEIHGKCENMTITPRGTTVTPTKETVSLGKHTVALGEKTEITGAMTMTSVGHQSMTPG
  EKALTPVGHQSVTTGQKTLTSVGYQSVTPGEKTLTPVGHQSVTPVSHQSVSPGGTTMTPVHFQTETLRQN
  TVAPRRKAVAREKVTVPSRNISVTPEGQTMPLRGENLTSEVGTHPRMGNLGLQMEAENRMMLSSSPVIQL
  PEQTPLAFDNRFVPIYGNHSSVNSVTPQTSPLSLKKEIPENSAVDEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002557
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002557.4
• RefSeq Size: 2258 bp
• RefSeq ORF: 2037 bp
• Locus ID: 5016
• UniProt ID: Q12889
• Cytogenetics: 1p13.2
• Protein Families: Secreted Protein
• MW: 75.4 kDa
• Gene Summary: This gene encodes a large, carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites located within threonine, serine, and proline-rich tandem repeats. The gene is similar to members of the mucin and the glycosyl hydrolase 18 gene families. Regulation of expression may be estrogen-dependent. Gene expression and protein secretion occur during late follicular development through early cleavage-stage embryonic development. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions. [provided by RefSeq, Jul 2008]