NDST1 (NM_001543) Human Tagged ORF Clone

CAT#: RC221638

NDST1 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1)

Product Images

Circular map for RC221638

• 

  Western blot validation of overexpression lysate (Cat# LY419873) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221638 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NDST1 protein (Cat# TP321638). The protein was produced from HEK293T cells transfected with NDST1 cDNA clone (Cat# RC221638) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDST1
• Synonyms: HSST; MRT46; NST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221638 representing NM_001543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGTCCCCGCAGGCTGTCCTTTTCCTGCTGT
  TCATCTTCTGCCTGTTCAGCGTTTTCATCTCGGCCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCC
  CTCGGCGGATGCCCCCGAGCCTGACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTC
  AAGCCTGTGCAGGCAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACT
  CGCAACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGATTGCGCC
  GGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCCTCATCATCTATGAGAAC
  ATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGCTGGACAAGTACTGTGTGGCCTACGGCG
  TGGGCATCATTGGCTTCTTCAAGGCCAATGAGAACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCT
  GTTCCTGCACTCAAACCTGGGCCTGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACG
  CGACCTAGCGAGGTGGAGAAAGGTGTGCTCCCCGGCGAGGACTGGACGGTTTTCCAGTCAAATCACTCCA
  CCTATGAGCCAGTGCTGCTGGCCAAGACGCGCTCGTCTGAGTCCATCCCACACCTGGGCGCAGACGCCGG
  CCTGCATGCTGCACTGCACGCCACTGTGGTCCAGGACCTGGGCCTGCACGACGGCATCCAGCGCGTGCTG
  TTTGGCAACAACCTGAACTTCTGGCTGCACAAGCTTGTCTTCGTGGATGCCGTGGCCTTCCTCACGGGGA
  AGCGCCTCTCCCTGCCATTGGACCGCTACATCCTGGTGGACATTGATGACATCTTCGTGGGCAAGGAGGG
  CACACGCATGAAGGTGGAGGACGTGAAGGCCCTGTTTGACACACAGAACGAACTACGCGCACACATCCCA
  AACTTCACCTTCAACCTGGGCTACTCAGGGAAATTCTTCCACACAGGTACCAATGCTGAGGACGCTGGGG
  ATGATCTGCTGCTGTCGTATGTGAAGGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCCCA
  CCTTTTCCACAACCAGTCCGTGTTGGCCGAGCAGATGGCCTTGAACAAGAAGTTCGCTGTCGAGCATGGC
  ATTCCCACAGACATGGGGTATGCAGTGGCGCCCCACCACTCGGGCGTGTACCCCGTGCACGTGCAGCTGT
  ACGAGGCTTGGAAGCAGGTGTGGAGCATCCGCGTGACCAGCACGGAGGAGTACCCCCACCTGAAGCCAGC
  CCGCTACCGCCGTGGCTTCATCCACAATGGCATCATGGTTCTCCCACGGCAGACCTGCGGCCTCTTCACA
  CACACCATCTTCTACAACGAGTACCCTGGCGGCTCCAGTGAGCTGGACAAGATCATCAACGGGGGCGAGC
  TCTTCCTCACCGTGCTCCTCAATCCTATCAGCATCTTCATGACGCACCTGTCCAACTATGGGAATGACCG
  CCTGGGCCTGTACACCTTCAAGCACCTGGTGCGCTTCCTGCACTCCTGGACGAACCTCCGGCTGCAGACA
  CTGCCCCCTGTGCAGTTGGCGCAGAAGTACTTCCAGATCTTCTCCGAGGAGAAGGACCCGCTCTGGCAGG
  ACCCCTGCGAGGACAAACGTCACAAAGACATCTGGTCCAAGGAGAAGACGTGTGACCGCTTCCCAAAGCT
  CCTCATCATCGGCCCCCAGAAAACAGGCACCACTGCCCTCTACCTGTTCCTGGGCATGCACCCTGACCTA
  AGCAGCAACTACCCCAGCTCTGAGACCTTTGAGGAGATCCAGTTTTTTAATGGCCACAACTATCACAAAG
  GCATCGACTGGTACATGGAGTTCTTCCCCATCCCTTCCAACACCACCTCCGACTTCTACTTTGAGAAAAG
  CGCCAACTACTTTGATTCAGAAGTGGCGCCCCGGCGGGCAGCAGCCCTCTTGCCCAAAGCCAAGGTCCTG
  ACCATCCTCATCAACCCCGCGGACCGGGCCTATTCCTGGTACCAGCACCAGCGAGCCCATGACGACCCAG
  TGGCCCTAAAGTACACCTTCCATGAGGTGATTACCGCCGGCTCTGACGCATCCTCGAAGCTGCGTGCCCT
  CCAGAACCGCTGCCTGGTCCCTGGCTGGTACGCCACCCACATCGAGCGCTGGCTCAGTGCCTATCACGCC
  AACCAGATTCTGGTCTTGGATGGCAAACTGCTTCGCACAGAACCTGCCAAAGTGATGGACATGGTGCAGA
  AGTTCCTTGGGGTGACCAACACCATTGACTACCACAAAACCTTGGCGTTTGATCCAAAGAAAGGATTTTG
  GTGCCAACTGCTTGAAGGAGGAAAAACCAAGTGTCTGGGCAAAAGCAAGGGCCGGAAATATCCCGAGATG
  GACTTGGATTCCCGAGCCTTCCTGAAGGACTATTACCGGGACCACAACATCGAGCTCTCCAAGCTGCTGT
  ATAAGATGGGCCAGACACTTCCCACTTGGCTACGAGAGGACCTCCAGAACACCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221638 representing NM_001543
  Red=Cloning site Green=Tags(s)
  
  MPALACLRRLCRHVSPQAVLFLLFIFCLFSVFISAYYLYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPL
  KPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYEN
  ILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVT
  RPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVL
  FGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIP
  NFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHG
  IPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFT
  HTIFYNEYPGGSSELDKIINGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKHLVRFLHSWTNLRLQT
  LPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLGMHPDL
  SSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFDSEVAPRRAAALLPKAKVL
  TILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGSDASSKLRALQNRCLVPGWYATHIERWLSAYHA
  NQILVLDGKLLRTEPAKVMDMVQKFLGVTNTIDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEM
  DLDSRAFLKDYYRDHNIELSKLLYKMGQTLPTWLREDLQNTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001543
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001543.5
• RefSeq Size: 7913 bp
• RefSeq ORF: 2649 bp
• Locus ID: 3340
• UniProt ID: P52848
• Cytogenetics: 5q33.1
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• MW: 100.7 kDa
• Gene Summary: This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]