BACH1 (NM_206866) Human Tagged ORF Clone

CAT#: RC221628

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BACH1 (Myc-DDK-tagged)-Human BTB and CNC homology 1, basic leucine zipper transcription factor 1 (BACH1), transcript variant 1



  "NM_206866" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol BACH1
Synonyms BACH-1; BTBD24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTGAGTGAGAACTCGGTTTTTGCCTATGAATCTTCTGTGCATAGCACCAATGTTTTACTCAGCC
TTAATGACCAGCGGAAGAAAGATGTGCTGTGCGATGTCACCATCTTTGTGGAGGGACAGCGGTTCCGCGC
TCACCGGTCCGTGCTGGCGGCATGCAGCAGTTACTTCCACTCAAGAATCGTAGGCCAGGCTGATGGAGAG
CTGAACATTACTCTTCCAGAAGAGGTGACAGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTG
CTAAACTGATTTTAAGTAAAGAGAATGTGGATGAAGTGTGCAAATGTGTGGAGTTTTTAAGTGTACATAA
TATTGAGGAATCCTGCTTTCAGTTTCTGAAATTTAAGTTTTTGGACTCCACTGCAGACCAGCAAGAATGC
CCAAGAAAAAAATGCTTTTCATCACACTGTCAGAAAACAGACCTTAAACTTTCACTTTTGGACCAGAGGG
ATCTAGAAACTGATGAAGTGGAGGAATTTCTGGAAAATAAAAATGTTCAGACTCCTCAGTGTAAACTCCG
CAGGTATCAAGGAAATGCAAAAGCCTCACCTCCTCTACAAGACAGTGCCAGTCAGACATATGAGTCCATG
TGCTTAGAGAAGGATGCTGCTCTGGCCTTGCCTTCTTTATGCCCCAAATACAGAAAATTCCAAAAAGCAT
TTGGAACTGACAGAGTCCGTACTGGGGAATCTAGTGTCAAAGACATTCATGCTTCTGTTCAGCCAAATGA
AAGGTCTGAAAATGAATGCCTGGGAGGAGTCCCGGAGTGTAGAGATTTGCAGGTGATGTTAAAATGTGAC
GAAAGTAAATTAGCAATGGAACCTGAAGAAACGAAGAAAGATCCTGCTTCTCAGTGCCCAACTGAAAAAT
CAGAAGTGACTCCTTTCCCCCACAATTCTTCCATAGACCCTCATGGACTTTATTCTTTGTCTCTTTTACA
CACATATGACCAATATGGTGACTTGAATTTTGCTGGTATGCAAAACACAACAGTGTTAACAGAAAAGCCT
TTGTCAGGTACAGACGTCCAAGAAAAAACATTTGGTGAAAGTCAGGATTTACCTTTGAAATCCGACTTGG
GCACCAGGGAAGATAGTAGTGTTGCATCTAGTGATAGGAGTAGTGTGGAGCGAGAAGTGGCAGAACACCT
AGCAAAAGGCTTCTGGAGTGACATTTGCAGCACGGACACTCCTTGCCAAATGCAGTTATCACCTGCTGTG
GCCAAAGATGGCTCAGAACAGATCTCACAGAAACGGTCTGAGTGTCCGTGGTTAGGTATCAGGATTAGTG
AGAGCCCAGAACCAGGTCAAAGGACTTTCACAACATTAAGTTCTGTCAACTGCCCTTTTATAAGTACTCT
GAGTACTGAAGGCTGTTCAAGCAATTTGGAAATTGGAAACGATGATTATGTTTCAGAACCCCAGCAAGAA
CCTTGCCCATATGCTTGTGTCATTAGCTTGGGAGACGACTCTGAGACGGACACCGAAGGAGACAGTGAAT
CCTGTTCAGCCAGAGAACAAGAATGTGAGGTAAAACTGCCATTCAATGCACAACGGATAATTTCACTGTC
TCGAAATGATTTTCAGTCCTTGTTGAAAATGCACAAGCTTACTCCAGAACAGCTGGATTGTATCCATGAT
ATTCGAAGAAGAAGTAAAAACAGAATTGCTGCACAGCGCTGTCGCAAGAGAAAACTTGACTGTATACAGA
ATCTTGAATCAGAAATTGAGAAGCTGCAAAGTGAAAAGGAGAGCTTGTTGAAGGAAAGAGATCACATTTT
GTCAACTCTGGGTGAGACAAAGCAGAACCTAACTGGACTTTGCCAGAAAGTTTGTAAAGAAGCAGCTCTG
AGTCAAGAACAAATACAGATACTCGCCAAGTACTCAGCTGCAGATTGCCCACTTTCATTTTTAATTTCTG
AAAAAGATAAAAGTACTCCTGATGGTGAACTGGCGTTACCATCAATTTTCAGTTTATCTGACCGGCCTCC
AGCAGTGCTGCCTCCCTGTGCCAGAGGAAACAGTGAGCCTGGCTACGCGCGAGGGCAGGAGTCCCAGCAG
ATGTCCACAGCCACCTCTGAGCAAGCTGGGCCTGCGGAACAGTGTCGTCAGAGTGGTGGGATCTCAGATT
TCTGTCAGCAGATGACTGATAAATGTACTACTGATGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221628 protein sequence
Red=Cloning site Green=Tags(s)

MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFHSRIVGQADGE
LNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQEC
PRKKCFSSHCQKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQDSASQTYESM
CLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIHASVQPNERSENECLGGVPECRDLQVMLKCD
ESKLAMEPEETKKDPASQCPTEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP
LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAV
AKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQE
PCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHD
IRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAAL
SQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ
MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_206866
ORF Size 2208 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_206866.3
RefSeq Size 5770 bp
RefSeq ORF 2211 bp
Locus ID 571
UniProt ID O14867
Cytogenetics 21q21.3
Protein Families Transcription Factors
MW 82 kDa
Gene Summary This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009]

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