BACH1 (NM_206866) Human Tagged ORF Clone

CAT#: RC221628

BACH1 (Myc-DDK-tagged)-Human BTB and CNC homology 1, basic leucine zipper transcription factor 1 (BACH1), transcript variant 1

Product Images

Circular map for RC221628

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BACH1 (Cat# RC221628, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1(Cat# TA504484). Positive lysates LY404144 (100ug) and LC404144 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY404144) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221628 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BACH1 protein (Cat# TP321628). The protein was produced from HEK293T cells transfected with BACH1 cDNA clone (Cat# RC221628) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BACH1
• Synonyms: BACH-1; BTBD24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221628 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGAGTGAGAACTCGGTTTTTGCCTATGAATCTTCTGTGCATAGCACCAATGTTTTACTCAGCC
  TTAATGACCAGCGGAAGAAAGATGTGCTGTGCGATGTCACCATCTTTGTGGAGGGACAGCGGTTCCGCGC
  TCACCGGTCCGTGCTGGCGGCATGCAGCAGTTACTTCCACTCAAGAATCGTAGGCCAGGCTGATGGAGAG
  CTGAACATTACTCTTCCAGAAGAGGTGACAGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTG
  CTAAACTGATTTTAAGTAAAGAGAATGTGGATGAAGTGTGCAAATGTGTGGAGTTTTTAAGTGTACATAA
  TATTGAGGAATCCTGCTTTCAGTTTCTGAAATTTAAGTTTTTGGACTCCACTGCAGACCAGCAAGAATGC
  CCAAGAAAAAAATGCTTTTCATCACACTGTCAGAAAACAGACCTTAAACTTTCACTTTTGGACCAGAGGG
  ATCTAGAAACTGATGAAGTGGAGGAATTTCTGGAAAATAAAAATGTTCAGACTCCTCAGTGTAAACTCCG
  CAGGTATCAAGGAAATGCAAAAGCCTCACCTCCTCTACAAGACAGTGCCAGTCAGACATATGAGTCCATG
  TGCTTAGAGAAGGATGCTGCTCTGGCCTTGCCTTCTTTATGCCCCAAATACAGAAAATTCCAAAAAGCAT
  TTGGAACTGACAGAGTCCGTACTGGGGAATCTAGTGTCAAAGACATTCATGCTTCTGTTCAGCCAAATGA
  AAGGTCTGAAAATGAATGCCTGGGAGGAGTCCCGGAGTGTAGAGATTTGCAGGTGATGTTAAAATGTGAC
  GAAAGTAAATTAGCAATGGAACCTGAAGAAACGAAGAAAGATCCTGCTTCTCAGTGCCCAACTGAAAAAT
  CAGAAGTGACTCCTTTCCCCCACAATTCTTCCATAGACCCTCATGGACTTTATTCTTTGTCTCTTTTACA
  CACATATGACCAATATGGTGACTTGAATTTTGCTGGTATGCAAAACACAACAGTGTTAACAGAAAAGCCT
  TTGTCAGGTACAGACGTCCAAGAAAAAACATTTGGTGAAAGTCAGGATTTACCTTTGAAATCCGACTTGG
  GCACCAGGGAAGATAGTAGTGTTGCATCTAGTGATAGGAGTAGTGTGGAGCGAGAAGTGGCAGAACACCT
  AGCAAAAGGCTTCTGGAGTGACATTTGCAGCACGGACACTCCTTGCCAAATGCAGTTATCACCTGCTGTG
  GCCAAAGATGGCTCAGAACAGATCTCACAGAAACGGTCTGAGTGTCCGTGGTTAGGTATCAGGATTAGTG
  AGAGCCCAGAACCAGGTCAAAGGACTTTCACAACATTAAGTTCTGTCAACTGCCCTTTTATAAGTACTCT
  GAGTACTGAAGGCTGTTCAAGCAATTTGGAAATTGGAAACGATGATTATGTTTCAGAACCCCAGCAAGAA
  CCTTGCCCATATGCTTGTGTCATTAGCTTGGGAGACGACTCTGAGACGGACACCGAAGGAGACAGTGAAT
  CCTGTTCAGCCAGAGAACAAGAATGTGAGGTAAAACTGCCATTCAATGCACAACGGATAATTTCACTGTC
  TCGAAATGATTTTCAGTCCTTGTTGAAAATGCACAAGCTTACTCCAGAACAGCTGGATTGTATCCATGAT
  ATTCGAAGAAGAAGTAAAAACAGAATTGCTGCACAGCGCTGTCGCAAGAGAAAACTTGACTGTATACAGA
  ATCTTGAATCAGAAATTGAGAAGCTGCAAAGTGAAAAGGAGAGCTTGTTGAAGGAAAGAGATCACATTTT
  GTCAACTCTGGGTGAGACAAAGCAGAACCTAACTGGACTTTGCCAGAAAGTTTGTAAAGAAGCAGCTCTG
  AGTCAAGAACAAATACAGATACTCGCCAAGTACTCAGCTGCAGATTGCCCACTTTCATTTTTAATTTCTG
  AAAAAGATAAAAGTACTCCTGATGGTGAACTGGCGTTACCATCAATTTTCAGTTTATCTGACCGGCCTCC
  AGCAGTGCTGCCTCCCTGTGCCAGAGGAAACAGTGAGCCTGGCTACGCGCGAGGGCAGGAGTCCCAGCAG
  ATGTCCACAGCCACCTCTGAGCAAGCTGGGCCTGCGGAACAGTGTCGTCAGAGTGGTGGGATCTCAGATT
  TCTGTCAGCAGATGACTGATAAATGTACTACTGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221628 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFHSRIVGQADGE
  LNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQEC
  PRKKCFSSHCQKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQDSASQTYESM
  CLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIHASVQPNERSENECLGGVPECRDLQVMLKCD
  ESKLAMEPEETKKDPASQCPTEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP
  LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAV
  AKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQE
  PCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHD
  IRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAAL
  SQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ
  MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206866
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206866.3
• RefSeq Size: 5770 bp
• RefSeq ORF: 2211 bp
• Locus ID: 571
• UniProt ID: O14867
• Cytogenetics: 21q21.3
• Protein Families: Transcription Factors
• MW: 82 kDa
• Gene Summary: This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009]