KCTD20 (NM_173562) Human Tagged ORF Clone

CAT#: RC221607

KCTD20 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 20 (KCTD20)

Product images

Western blot validation of overexpression lysate (Cat# LY406473) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221607 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: KCTD20 (NM_173562) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD20
• Synonyms: C6orf69; dJ108K11.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221607 representing NM_173562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTTCACCGTGGCAGTGACAGTGACAGGTTATTGCGGCAGGAGGCCAGCTGCTTAGTGGATGATA
  CTTTAGCTGTAGCCCAAGAAAAAGAAGCAAACAGCCTGGCTTCATCTGGTCCTCATAATCTTACTTATCC
  TCTAGGTCCCAGGAATGAAGACCTCTCACTTGACTATGCCTCTCAGCCAGCAAATCTTCAGTTCCCTCAC
  ATAATGCCCCTTGCTGAAGACATCAAAGGTTCTTGCTTCCAAAGTGGGAATAAACGGAACCATGAACCTT
  TTATTGCTCCAGAAAGATTTGGAAACAGTAGTGTGGGCTTTGGCAGTAATTCCCATTCCCAAGCACCAGA
  GAAAGTGACGCTTCTTGTAGATGGCACACGTTTTGTTGTGAATCCACAGATTTTCACTGCTCATCCGGAT
  ACCATGCTGGGAAGGATGTTTGGACCAGGAAGAGAGTACAACTTCACTCGGCCCAATGAGAAGGGAGAGT
  ATGAGATTGCTGAAGGCATCAGTGCAACTGTATTTCGCACAGTGCTGGATTATTACAAAACCGGTATCAT
  CAATTGTCCTGATGGCATCTCTATCCCAGATCTTAGAGATACTTGTGATTATCTCTGCATTAATTTTGAC
  TTCAACACTATCCGATGTCAAGATCTGAGTGCTTTACTCCATGAACTGTCTAATGACGGTGCTCATAAGC
  AGTTTGATCACTACCTCGAAGAGCTCATCTTGCCCATCATGGTGGGCTGTGCCAAGAAAGGAGAACGAGA
  GTGCCACATTGTTGTGCTGACGGATGAGGATTCTGTGGACTGGGATGAAGACCACCCTCCACCAATGGGG
  GAGGAATATTCCCAAATTCTTTATAGCTCCAAGCTCTACAGATTCTTCAAATATATTGAGAATAGGGATG
  TTGCAAAAACAGTGTTAAAGGAACGGGGCCTAAAAAACATTCGCATTGGAATTGAAGGTTACCCTACCTG
  TAAAGAAAAAATTAAGAGAAGGCCTGGCGGCCGGTCTGAAGTCATCTATAATTATGTACAACGCCCCTTC
  ATCCAGATGTCATGGGAAAAGGAAGAAGGGAAGAGTCGCCATGTGGATTTCCAGTGTGTTCGAAGCAAAT
  CCCTCACGAATCTGGTAGCTGCTGGAGATGATGTCTTGGAGGACCAGGAGATATTAATGCATCACCCACC
  CCAAGTGGATGAACTTGACCGGCTAAATGCCCCACTTTCTCAGATGGCTTCTAACGACTTTCAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221607 representing NM_173562
  Red=Cloning site Green=Tags(s)
  
  MNVHRGSDSDRLLRQEASCLVDDTLAVAQEKEANSLASSGPHNLTYPLGPRNEDLSLDYASQPANLQFPH
  IMPLAEDIKGSCFQSGNKRNHEPFIAPERFGNSSVGFGSNSHSQAPEKVTLLVDGTRFVVNPQIFTAHPD
  TMLGRMFGPGREYNFTRPNEKGEYEIAEGISATVFRTVLDYYKTGIINCPDGISIPDLRDTCDYLCINFD
  FNTIRCQDLSALLHELSNDGAHKQFDHYLEELILPIMVGCAKKGERECHIVVLTDEDSVDWDEDHPPPMG
  EEYSQILYSSKLYRFFKYIENRDVAKTVLKERGLKNIRIGIEGYPTCKEKIKRRPGGRSEVIYNYVQRPF
  IQMSWEKEEGKSRHVDFQCVRSKSLTNLVAAGDDVLEDQEILMHHPPQVDELDRLNAPLSQMASNDFQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173562
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173562.5
• RefSeq Size: 5032 bp
• RefSeq ORF: 1260 bp
• Locus ID: 222658
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• MW: 47.3 kDa
• Gene Summary: Promotes the phosphorylation of AKT family members.[UniProtKB/Swiss-Prot Function]